EMAGE mouse gene expression spatial database

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EMAGE:7201

H2afx H2A histone family, member X ( MGI:102688)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002718. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002718_01	euxassay_002718_02	euxassay_002718_03	euxassay_002718_04

euxassay_002718_05	euxassay_002718_06	euxassay_002718_07	euxassay_002718_08	euxassay_002718_09

euxassay_002718_10	euxassay_002718_11	euxassay_002718_12	euxassay_002718_13	euxassay_002718_14

euxassay_002718_15	euxassay_002718_16	euxassay_002718_17	euxassay_002718_18	euxassay_002718_19

euxassay_002718_20	euxassay_002718_21	euxassay_002718_22	euxassay_002718_23	euxassay_002718_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7201_wholemount_strong.wlz

7201_wholemount_moderate.wlz

7201_wholemount_weak.wlz

7201_wholemount_possible.wlz

7201_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7201_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5891
Entity Detected:	H2afx, H2A histone family, member X ( MGI:102688)
Sequence:	sense strand is shown >T5891 GTTCGTGGTCTCTCAGCGTTGTTCGCAGCTCTTCTACCTCGTACACTATGTCCGGACGCGGCAAGACCGG CGGCAAGGCCCGCGCCAAGGCCAAGTCGCGCTCTTCACGCGCCGGCCTTCAGTTCCCTGTAGGCCGCGTA CACCGGCTGCTGCGGAAAGGCCACTACGCCGAGCGCGTGGGCGCAGGCGCGCCGGTGTACCTGGCAGCGG TGCTCGAGTACCTCACTGCCGAGATCCTGGAGCTGGCGGGCAACGCGGCCCGCGACAACAAGAAGACGCG CATCATCCCGCGCCACCTGCAGCTGGCCATCCGCAACGACGAGGAGCTCAACAAGCTGCTGGGCGGCGTG ACCATCGCGCAGGGCGGCGTCCTGCCCAACATCCAGGCCGTGCTGCTGCCCAAGAAGAGCAGCGCCACCG TGGGGCCCAAGGCGCCGGCGGTCGGCAAGAAGGCCTCGCAGGCCTCTCAGGAGTACTGAGGGGGCCCGCG CCGAGGCCGCCCAGCCCTCCCCACACCTCCACAAAGGCCCTTTTAAGGGCCACCACCTCCCTCACAGAAA GAGCTGAGCCACTTCGGGCTGCGGCGGCTTGCCGCGTCCCCATCCTTTCTCTTTCCCTCCTCTCCCCTCC CCGCCCTCGCGCCACTCTTCCCCTTACCCCGCACGGCCTCCGGTTCTGCCACGGAGGACGCGGGCGCCGC GGCACCTGCCCATCCCGGAGCTCGTGACATCGCTTCGCCGTTCTCGGTTGTGGACGACGGCTCCGTGCGG AGAGGCTGCGGCGCCTTCCCGGAGTCTCGGCCCCTCGCTCAGACGTCCGGCCGCGGTGGGCTTGAAGGTT AGTCCCTCCGCAGCTACAGCCCCGGCGTCTTTGCTTCAGCTTGGTGCTTAGCCCTCGCCTGGACCTTTGA AAAGTGACTTGATTGCCGGGCTTAGAGGAGGAGAAATGGATCACATTGTTTCCTTCGGTGTCACCGTTGC TGGCCTCATACCAGTTGACCCTGTCGCCATTCGTAGGCCAGTGTGGTGTGGCGGGGCCAAAAATGGTTGT AATGTGAACCCAGTTTCTCTAGGAATACCTCCCGGCAAGGGTCAGAGAGACGCTTACCGGCCTGTGGACA AGAGTTCTATACCTGCCGCAGGCGACTGGCAGCCGGGCCTAGCCATCCATCCCCTCTTCCCCAGCGACTC AACTACAACCCAAACACCTAGATACCAGCACAAGCCGGTGAATCCCTGTCTGGACTGAGCCTCTGGCTTC TGAACTGGAACTTTTGCAGCTATTACAAGTTTAGAACCTTAAGTGGGGAGATTTAATGGGCTAATTTTAT TAAAGGATCGTTTGGTTTTGTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3156946 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3156946 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7200 same embryo
	EurExpress:euxassay_002718 same experiment
	MGI:4825304 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source