EMAGE mouse gene expression spatial database

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EMAGE:7305

Vps11 vacuolar protein sorting 11 (yeast) ( MGI:1918982)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004377. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004377_01	euxassay_004377_02	euxassay_004377_03	euxassay_004377_04

euxassay_004377_05	euxassay_004377_06	euxassay_004377_07	euxassay_004377_08	euxassay_004377_09

euxassay_004377_10	euxassay_004377_11	euxassay_004377_12	euxassay_004377_13	euxassay_004377_14

euxassay_004377_15	euxassay_004377_16	euxassay_004377_17	euxassay_004377_18	euxassay_004377_19

euxassay_004377_20	euxassay_004377_21	euxassay_004377_22	euxassay_004377_23	euxassay_004377_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7305_wholemount_strong.wlz

7305_wholemount_moderate.wlz

7305_wholemount_weak.wlz

7305_wholemount_possible.wlz

7305_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7305_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5265
Entity Detected:	Vps11, vacuolar protein sorting 11 (yeast) ( MGI:1918982)
Sequence:	sense strand is shown >T5265 CACGTGATTTCGCTTCCGGAGGCGGGGGCCTTCGGCAAGATGGCGGCCTACCTGCAGTGGCGGCGTTTCG TTTTCTTCGAGAAGGAGCTGGTGAAGGAGCCGCTGGGTAATGATGGGGCTGCTCCCGGTGCCGCGCCTGT GTCGGGATCCGCTGCTTCCAAGTTCCTTTGCCTCCCTCCTGGCATCACTGTCTGCGACTCAGGCCGCGGG AGCCTGGTCTTTGGAGATATGGAAGGCCAGATCTGGTTCTTGCCACGTTCTCTACAGCTTACAGGGTTCC AGGCCTACAAACTACGGGTGACACACTTATATCAGCTGAAGCAGCACAATATTCTGGCATCGGTTGGTGA GGATGAAGAGGGTATTAACCCTCTGGTAAAGATCTGGAACCTGGAAAAGAGAGACGGTGGCAATCCACTC TGCACCCGGATTTTCCCTGCCATCCCAGGGACAGAGCCAACTGTTGTGTCTTGTTTGACGGTCCATGAGA ATCTCAACTTTATGGCCATTGGGTTCACAGATGGCAGTGTCACCCTGAACAAGGGAGATATTACCCGGGA CCGGCACAGCAAGACGCAGATTTTGCACAAGGGCAACTATCCCGTTACTGGGCTGGCCTTTCGCCAAGCA GGAAAGACCACCCACTTGTTTGTCGTGACAACTGAAAATGTTCAGTCTTATATAGTTTCTGGAAAGGACT ACCCTCGTGTGGAGTTGGACACCCATGGTTGTGGCCTGCGCTGTTCAGCCCTGAGCGACCCTTCTCAAGA CCTGCAGTTCATTGTTGCTGGGGATGAGTGTGTCTACCTTTACCAGCCTGATGAACGTGGACCCTGCTTT GCCTTTGAGGGCCATAAGCTCATCGTGCACTGGTTTAGAGGCTACCTTGTCATTGTCTCCCGTGACCGGA AAGTTTCTCCCAAGTCCGAGTTTACCAGCAGGGACTCACAGAACTCCGACAAGCAGATTCTCAACATCTA TGACCTGTGCAACAAGTTCATAGCCTACAGCGCTGGGTTTGAGGATATAGTGGATGTGCTGGCTGAGTGG GGCTCCTTGTACGTGCTGACGCGGGATGGGCGGGTCCACGCATTGCAGGAGAAGGACACACAGACCAAAC TGGAGATGCTGTTTAAGAAGAATCTATTTGAGATGGCAATTAATCTGGCTAAGAGTCAACATTTGGACAG TGATGGGTTGGCTCAAATCTTCATGCAGTATGGAGACCATCTCTACAGCAAGGGCAACCATGATGGGGCT GTGCAGCAGTATATCCGAACCATTGGAAAGTTGGAGCCATCCTATGTGATCCGGAAGTTTCTGGATGCCC AGCGCATCCACAACTTGACCGCCTACCTGCAAACCCTGCATCGGCAGTCTCTGGCTAATGCTGATCATAC CACCCTGCTTCTGAACTGCTATACCAAGCTCAAGGACAGCTCGAAGCTGGAGGAATTCATCAAGACAAAG AGTGAGAGTGAAGTCCACTTTGATGTAGAGACAGCCATCAAGGTCCTCCGGCAGGCTGGCTACTACTCCC ATGCCCTCTATCTGGCTGAGAATCATGCACATCATGAGTGGTACTTGAAGATCCAGCTAGAGGACATTAA GAACTATCAGGAAGCCCTTCGGTATATCGGGAAGCTGCCTTTCGAGCAAGCGGAGAGTAACATGAAACGC TATGGCAAGACCCTCATGCACCACATACCGGAGCAGACAACCCAGTTGCTGAAAGGCCTCTGCACTGACT ATCGACCCAGTCTGGAAGGCCGAGGCGATCGGGAGGCTCTGAGCTGCCGGGCCAGTTCTGAGGAGTTCAT CCCCATCTTTGCCAACAACCCACGAGAGCTGAAAGCCTTCCTAGAACACATGAGTGAGGTGCAGCCGGAC TCACCGCAGGGCATCTATGACACGCTCCTGGAGCTGCGGCTGCAGAACTGGGCCCATGAGAAAGATCCCC AGGCCAAGGAGAAACTTCATGCAGAGGCCATCTCCCTGCTGAAGAGTGGCCGATTCTGTGACGTGTTTGA CAAGGCATTGGTTCTGTGCCAGATGCACGACTTCCAGGATGGGGTCCTTTACCTCTATGAGCAGGGGAAG CTGTTCCAGCAGATCATGCATTACCACATGCAGCACGAGCAGTACCGGCAGGTGATTGCCGTGTGTGAGC GCCATGGAGAGCAGGAGCCCTCCCTGTGGGAACAGGCCCTCAGCTACTTCGCCCGCAAGGAGGAGGACTG CAAGGAGTATGTGGCGGCTGTGCTCAGGCACATTGAGAACAAGAGCCTCATGCCGCCTCTTCTAGTGGTG CAGACCCTGGCCCACAACTCCACAGCTACCCTGTCTATCATCCGAGATTACCTGGTCCAAAAACTGCAGA AACAGAGCCAGCAGATTGCACAGGATGAGCTCCGGGTGCGGCGATACAGAGAGGAGACCACTCGCATCCG CCAGGAAATCCAGGAGCTCAAGGCGAGTCCAAAGATTTTCCAGAAGACCAAGTGTAGCATCTGTAACAGT GCCTTGGAGCTGCCCTCAGTCCACTTCCTGTGTGGCCACTCCTTCCACCAACACTGCTTTGAGAGTTACT CAGAAAGTGATGCTGACTGCCCCACCTGCCTCCCTGAGAACCGCAAGGTGATGGATATGATCCGGGCCCA GGAACAGAAACGAGATCTCCACGATCAGTTCCAACATCAGCTCAAGTGCTCGAATGACAGCTTCTCTGTG ATTGCGGACTACTTTGGCCGAGGGGTTTTCAACAAGTTGACTCTGCTGACCGACCCTCCTACAGCCAGAC TGACCCCCAGCCTGGAGGCTGGACTGCAGCGGGACCTGCTGATGCACTCCAGGAGGGGAACTTAGTTAGG CAGCTCCTGGGGAAGCCTATATCCCTGGGCCTCTGAAGCACAGACGTAGGCCTGAGGAAGAGATGGTAGC AGACATGACCACAGTTCCGGAGCCTCAGGACTCAAGAAGGGACTCTGCTTTTCCTGCCTCCTCCTTTTGC CAGCCTGCTGTCCCTCCTGCTGACCAGCAGTATAGATTCTTCTACACCAGTGAGGAAGGCCACCTCAGCA ACCTCTCTGGACAATAGCTGCTTTATTCTCTACTCAAGGGCACCAGGCTGTGCTTGGGTCCTTGCTCCCA GAGCCTATAAATAAAAGTATAATAGTATGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5360111 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5360111 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7309 same embryo
	EMAGE:7306 same embryo
	EMAGE:7307 same embryo
	EMAGE:7308 same embryo
	EMAGE:7304 same embryo
	EurExpress:euxassay_004377 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source