EMAGE mouse gene expression spatial database

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EMAGE:7369

Hnrpll heterogeneous nuclear ribonucleoprotein L-like ( MGI:1919942)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004494. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004494_01	euxassay_004494_02	euxassay_004494_03	euxassay_004494_04

euxassay_004494_05	euxassay_004494_06	euxassay_004494_07	euxassay_004494_08	euxassay_004494_09

euxassay_004494_10	euxassay_004494_11	euxassay_004494_12	euxassay_004494_13	euxassay_004494_14

euxassay_004494_15	euxassay_004494_16	euxassay_004494_17	euxassay_004494_18	euxassay_004494_19

euxassay_004494_20	euxassay_004494_21	euxassay_004494_22	euxassay_004494_23	euxassay_004494_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7369_wholemount_strong.wlz

7369_wholemount_moderate.wlz

7369_wholemount_weak.wlz

7369_wholemount_possible.wlz

7369_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7369_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6475
Entity Detected:	Hnrpll, heterogeneous nuclear ribonucleoprotein L-like ( MGI:1919942)
Sequence:	sense strand is shown >T6475 GCGAAGCTGGCGGAGGGAGAGGCTGGAGGCTGGCTGAGAGATAAAGGGAAGCGTCCCCTCAGCCCGCGCT CCCTTCTCAGTCACGGGCTCCTGGAGCGCGCCGCGGAGGACGCCGGGCCAGCTCAGACCCGCGCCGAGGA GGCGCAGCCCGCGGCGGGGACCGATCAGCGACATCTGAGGCCCGTCTCCCGCGCCGTCTCCACCGCCGCC GCCATGTCCTCCACCTCCTCCTCCTCCCCCAAGGAGGAGACGTACGAGGAGGACCGGGAGTTCGAGAGCC AGGCCAAGCGCCTCAAGACCGAGGAGGGAGAGATCGTCTACTCGGCCGAGGAGAGCGAGAATCGCCAGGA AGCGACGCCTCAGGCGGGGAGCGATAGCGACAGCGGCGGCGGCGACGGAGGCGACGGAGACGGAGGCAGC GGCGGCGGCGGCGACGGCGAGGAAGGCGAAGGTGGAGAAGAAGGCGACGAAGGCGACGGCGATGAGGGAG GGAGCGGCGGCGACGAAGGCGGCAGCGGCGGTGGCGGCGGCGGCCCTCGGAGCATGCCCCTGTCCACGGA GGGAGGTGGAAGTCATCATAAAGTTTCTGTTTCCCCTGTTGTCCATGTTCGAGGGCTCTGTGAATCTGTT GTGGAAGCTGACCTTGTGGAGGCACTGGAGAAATTTGGGACAATATGCTATGTGATGATGATGCCATTTA AACGACAGGCTCTCGTGGAGTTTGAAAATATAGATAGTGCCAAAGAATGTGTGACATTTGCTGCAGATGT GCCTGTGTACATTGCTGGCCAGCAGGCTTTCTTCAACTATTCTACAAGTAAAAGAATCACTCGGCCAGGA AATACCGACGATCCATCAGGGGGCAACAAAGTGCTCTTGCTCTCTATTCAGAATCCTCTTTACCCAATTA CGGTGGATGTTCTGTATACTGTATGCAACCCTGTTGGAAAAGTACAGCGTATTGTTATCTTCAAGAGAAA TGGGATACAAGCAATGGTTGAGTTTGAGTCAGTCCTTTGCGCCCAGAAGGCTAAAGCAGCACTCAATGGA GCTGATATATATGCTGGATGTTGCACACTAAAAATTGAATATGCAAGGCCAACTCGTCTAAATGTTATTA GGAATGACAATGACAGTTGGGACTACACTAAACCTTATTTGGGAAGACGAGATAGAGGAAAGGGTCGCCA GAGACAAGCCATTTTGGGAGACCATCCTTCTTCGTTTAGACATGATGGCTATGGATCGCATGGTCCGCTA CTGCCTTTACCAAGTCGTTATAGAATGGGCTCTAGAGATACACCTGAACTTGTTGCTTATCCGTTACCAC AAGCGTCTTCCTCTTACATGCATGGAGGCAGTCCGTCTGGCTCCGTTGTAATGGTTAGTGGATTACATCA GCTCAAAATGAATTGTTCAAGAGTCTTCAACCTGTTCTGCTTGTATGGAAATATTGAAAAGGTAAAGTTT ATGAAGACCATTCCTGGCACAGCACTTGTAGAAATGGGTGACGAGTATGCTGTGGAAAGAGCTGTCACCC ACCTTAATAATGTCAAACTCTTTGGGAAAAGACTTAATGTTTGTGTGTCTAAACAGCACTCAGTTGTTCC CAGTCAAATATTTGAGCTAGAGGATGGTACGAGCAGCTACAAAGATTTTGCAATGAGCAAAAATAATCGC TTTACAAGTGCTGGACAAGCATCTAAGAATATAATCCAGCCACCGTCATGTGTGCTGCATTATTACAATG TTCCGCTGTGTGTCACAGAAGAGACCTTCACAAAGTTGTGTAATGACCACGAAGTTCTTCCGTTCATCAA ATATAAAGTGTTTGACGCGAAAGCTTCTGCGAAAACTCTGTCTGGGCTATTGGAGTGGAAGTGCAAAACT GATGCCGTGGAAGCTCTCACTGCGCTCAATCACTACCAGATACGAGTCCCGAATGGCTCCAACCCCTATA CGTTGAAGCTTTGCTTTTCTACATCATCCCATTTATAAGAAGAGAAGAGCATGTTAGAATTTATGTTCAT CTTTATTGCAGTTTTAAAGCTATACTTCATCCATAATAATCTAAAATGGTTGGTCTAATGTTGCCTTGCT TACTATAAGATCCTGTTCTGTAATAAACATATTTTGCCTCAAGTAAGTTTGTTGTTAACTTAAATATTGT TTTCACCTTAAGATAGAGTACCATAATGTAGACTGTCTACAGCTTCTTTGTAGATTATACAAATGTATGG TTTCTAACATTATTACGAATTTTGTTCCATGGTAAAATATATTTGCATTCTTAATAGTAATTATCTGCAG ATGTTTTTCCATTGTGTATTAGATTAATGCAGGTGAAAGTATTGCATTTTAATACCGAATTCCTACAATA GATGATTGAGTCTGTTACAGTTACTCATATTAAATATAATTTGTATTTATTATTTAATCAAATTTGAGAA TGACGCTGCATATGTTGACTTTCAGGAACTACAGACTTAGCTGACGTCAATCTCTAAAAGGCTTAATAGA CACGAACACACTTGTATGGTATGCACTCAAACTCAGATTGCTGTTTCTTTTAAAAAGTCATCATTTGTAT TGACATGCTTCTGTTCCTAGTTCCAGTTTGGAGGTTTTATAAAGTTATAATAATAAGTTAATGTGTTCAT CTTCATTATTTTGCATGTGACCTAATCTGAAAAGTACATGTAGTATTTGACATTGATATTTTAATACTGT AGAAGCTAAAATTAACAATTCTTCTATATAATAATCTGGGTTCCATAAATGAGTTTCAGAGAACTTGTGA GCCCTTCCAAAATTGTGTGTAGAGTTTGGAATATATGCATTTTTATTTCTTAATCTTTGATCCATCATAA TATATGTATTTTTCTATATGGTGTAAGATTTAAGAGTTAATTTTATTTTTGATCAAAATAGCTATTTGTC TCAACACCATTTATTAAACATTCTCCCTCTGGGTTTGTTTCTTTCTCTTTTAAGTGAAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3587716 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3587716 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7368 same embryo
	EMAGE:7367 same embryo
	EMAGE:7365 same embryo
	EMAGE:7366 same embryo
	EurExpress:euxassay_004494 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source