EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7415

Kbtbd8 kelch repeat and BTB (POZ) domain containing 8 ( MGI:2661430)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005231_01

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7415_wholemount_possible.wlz

7415_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7415_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7633
Entity Detected:	Kbtbd8, kelch repeat and BTB (POZ) domain containing 8 ( MGI:2661430)
Sequence:	sense strand is shown >T7633 AAGAGGTAATCCGTAAGTCTTCCCCAACACCGAATGGGATTCCATCTTCAGACACAGCCAACGATACCAT GGATCCTTTCCATGCTTGCAGTATTCTTAAGCAACTCAAAACAATGTACGATGAAGGACAGTTGACAGAC ATTGTAGTGGAAGTGGATCACGGGAAAACATTTTCCTGTCATAGAAACGTTCTTGCTGCAATCAGCCCCT ACTTCAGATCCATGTTCACTAGCGGCCTTACAGAAAGTACTCAGAAAGAAGTTCGAATCATTGGTGTTGA AGCTGAATCAATGGATTTAGTATTGAATTATGCCTATACCTCTAGAGTTATTCTGACAGAGGCCAATGTT CAAGCCTTGTTCACTACAGCTAGCATCTTCCAAATTCCTTCCATACAAGACCAGTGTGCTAAGTATATGA TTAGTCATTTGGATCCACAGAATTCTATTGGAGTCTTTATCTTTGCTGATCATTATGGTCATCAGGAGCT TGGAGATCGATCAAAAGAATATATCCGTAAAAAGTTTCTGTGTGTCACCAAAGAACAAGAGTTTCTCCAA CTGACAAAAGACCAACTCATAAGCATACTAGACAGTGACGATTTAAATGTGGATCGGGAAGAACATGTTT ATGAAAGCATTATAAGGTGGTTTGAACATGAGCAGAGTGAAAGAGAAGTACACCTTCCAGAGATTTTTGC CAAATGTATACGTTTTCCTCTAATGGAAGATACATTTATCGAGAAAATTCCTCCTCAGTTTGCACAGGCC ATAGTCAAAAGTTGTGGAGAACCCTCCAACACCAGTGGCTGTACACAGAGGCTTGGAATGACTGCTTCTG AAATGATCATATGTTTTGATGCTGCCCACAAACACTCAGGAAAGAAGCAAACAGTGCCTTGTCTAGATAT CGTCACAGGAAGAGTGTTTAAACTATGCAAACCACCAAATGACCTAAGAGAAGTTGGGATTCTTGTGTCA CCAGATAATGACATATACATTGCAGGAGGCTACAGGCCAAGCAGCAGTGAGGTGTCCATCGACCATAAAG CAGAAAATGATTTTTGGATGTATGACCATTCCACCAACAGATGGCTTTCCAAGCCGTCCTTGCTTCGGGC CAGAATAGGCTGCAAGCTAGTATACTGCTGTGGTAAAATGTATGCAATTGGAGGGCGTGTTTACGAAGGT GATGGAAGAAACTCACTGAAGTCTGTGGAGTGCTATGATAGCAGAGAGAATTGCTGGATGACTGTGTGTG CCATGCCAGTTGCAATGGAGTTTCATAATGCTGTGGAGCACAAAGAGAAAATCTACGTTTTACAGGGGGA ATTTTTCCTCTTCTATGAGCCTCAAAAAGACTACTGGGGATTTTTAACCCCCATGACTGTGCCTCGAATC CAGGGCTTAGCAGCTGTGTACAAAGACTCAATCTACTACATAGCTGGAACCTGTGGAAATCATCAGCGTG TGTTTACTGTTGAAGCCTATGATATAGAGCTAAATAAGTGGACTCGTAAAAAGGACTTTCCGTGTGATCA GTCTATAAATCCATACCTGAAGCTAGTGCTTTTCCAGAATAAACTCCATTTATTTGTGCGAGCTACTCAA GTGACTGTTGAAGAACATATCTTCAGAACCAGCAGGAAAAATTCTCTTTACCAATACGATGACATTGCTG ACCAGTGGATGAAAGTGTATGAGACCCCAGATCGGCTGTGGGACCTTGGCCGCCATTTTGAGTGTGCGGT GGCTAAACTCTATCCTCAGTGTCTTCAGAAAGTTCTTTAAATAAGCAGCAGGCCAAAGCACGCCCCTGGC ATCTCATACTGAGGAAGCTGGCTTAGGAAATGCTGTCGCCGTTGAAGAAAGCTAACAGAGTCTTGTATTC AGAAATGGGTGCTCATAAAGACTGTGGTGTGGAGAGGGATTTGATTTTACCTTCATGATATTTTCAGTTC AGTCAGGAAAGGGAACAAAGTGCGGTTATCAGCATTTTTCCACCCCTGGCATAAAAGTATCATCCTAGAG TTTTGTGATAAATAGTTGCTGAGAAACCAGGTGAATTCAAGACAACTATGCACTCTTGAAAAGCAGAATA TTCCTGGGTAGAGACATCCAAACTGGTTAACGTGACTTTCTTTTAACTACGGGTAACAATATGAGGCAAT ATCATTTATTTAGCTGTGATCTCTAACACAGAGAAGCACTAACCTAGATCCTCAGTCTTACTATTTATAT CTATTTACATATATATTTTTGTGTACTGTTTTCAAGTGTACGAGATTTAAATGTGCTGTCAGCAGACGGA AGGGAAACTCGGTTTTCAGAGCGAACTTTTAGTCCGATTGTTCCATGTAACTTTTAAGTTAAGCTGCAAG GTTAAAGTGGCAGTTTTCTGTCCATAACTTTTTCAAGTGCTAACACTGTCTCATTTTTTAAAATCCCAGA AAGGGCTCAGATTTGCAGGTGACTGGTGCTCGTAAGTTCATTCATGTGTAGTTAGATCAACTTAAATGGT TACCGAGTCTCTGTTTATCTTTCAGGTTAGCATGCTAGTTCCATGGTCCTGGCACTTTACCTTGTGATTC CATGAGGTCTGCTATTTGTGTGATAGGATATGGCCGTTGGCCATTGGTCTAGCTGGATTCAGTTTTACAA TTCCAAGTTTGCACTAACATGGGCTGTTTTGTTGCCCAAACTCTCTTTCCATCCTCTACCTTTCCAGTCA TTGTTGGCATCGTGAAAGTTAACTAATAATGTGAAGTTTTAACACCTACTTATAAAATTCTGTTTTCTAG AAACAAGAGTCTTGGAGTGGTCTAATAATTAAGTGGCTTATAAGGTTCATATTATGGTTACAACCTCTCT ATTTTTTTGCACTCAACAGTGATGAATATTTTTATGTTGAAACACTTGTAGCTGAAGGCGATCTAGAAGG AAGGATGAGTGAGTGAACAGGTCAAAGGTTTCTGTGCTCTAGGTACTAAGGCGCTGTTCATAGTGAACTG ATAAGTTTCTCACAGTTAGTTAGGGATATTAACATAAACTCCTTTTCACCTAAGGCAGGTATTTAGGTTG ATAACATACTCATTACTATGCCTACTATAACTGATAAAGAATATTACACATAAGGCAGAGCCTGTCCTCA GTCAACACATTTTCTTTAAGAGACTCAATTTGATTCTCTTGAAGAAACCTGGCAAGATTTTGTATTGGAA TAGAAAAAAGTCTTGCACAACGTGTAAGGTGATAGATACTGTGAAACAAATGGAAAATATTGCCTCTTGC ATCACATACCTCCTTTTCCAGAAACTTTATAGACTGCATTACAAAGGCCCTACAAGGAGAGAGTTCTCGA AGCAGTTCAAGTGGACAGCATTCACAGATTGTTCATGTGTCCTCCACAGCTGGATGCGTGGCTTCTCCTA ATCTCTTTGTAAACTTTGTCAGCCAATATTTTTTTAAACCTCTCTAGTTGTCTTATGGTTTTGTTTTTTT TGGGTTTTTTTCATGCTGTTTTAGGGTTATTTCTGTGAATTTTTTGAATTATAGTTTTGTATTGCTAATG AGAGGGAAGAGAATTTTTACAAGAGATATCCATGTAATTTATTTTTGAAAGCTTTGTTGAGTATCTAAGA TTTCCTGCCACTTTGACAATCTCTGTATTTAGGAAAATTCATTACTTGAATACATGATGTAGAACATTGA GTTAGCAATGTACATGTACCGTACGATATGTAAGAGAACAATACACTGTGGCTAATTCTATACTAGATTT ATTCTGTTGGTCTGTACAACAGTTGGTCCTTTTGTCATAACTGTGTCTATTGAGGGAATCATTTCAGAAT AGTGTATCAAAGCCAAGGTTTAGAATTTGCTACAGGAGTAGAATATATATTGAATTTTGTATGAATTATT TGTTTACATTACATAGCTGGGATATTTTGCCACTTTTAAAATGACTTTAGAAAAGGTCCTAGGAAACTTA AGATTAGTGATGTGTGTGGGTTTATGTGTAGAAATTCAAGGTACATTCTCATAGTCTGAATATAAGTAAA AGGCACAAAGGCTACAGCAGGATTCAAAGATCTAACAAATGCCAATCCCACTTTAACTATGTTTCTGCAT TTGAATGTACACAGCACTTTTCCTATATAGTTCTATACAAAGAAAACAGAACTAGGTATAACTGTGTTGT AGGATTGAAGAAATCATTATTTATGTCTGTCTTTGTTTCTTGTTATCCTACAAAGTTGATGTGTAAACAT CAAGTTGATTTCATTGGTGCATGAGGAAAAGTGGATGTGATCTGTGAGGAACCAGAGTCCCATGGGAAGA GTTTAAGATGGCATTCAATATTCAGTGCTCAGGTATGTAGTAAGTACTGTAGTCCTATGGGGCAAATGTG TAGATATTTTTAAACATTTTGCCGTAATTGCACAATTTTTGCATTTTTACCTGATGTCATTGTTTCTTGT AATAAAATCTTTTCTGATTGAAAACTGCCAGTGTTGTAAACTGATCTCAAGAATGAATTTTGCAAAATTA AAATTTGGTGAAAATCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30459462 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30459462 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7413 same embryo
	EMAGE:7414 same embryo
	EMAGE:7416 same embryo
	EMAGE:7412 same embryo
	EMAGE:7417 same embryo
	EurExpress:euxassay_005231 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source