EMAGE mouse gene expression spatial database

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EMAGE:7417

Lrrc8e leucine rich repeat containing 8 family, member E ( MGI:1919517)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005224. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005224_01	euxassay_005224_02	euxassay_005224_03	euxassay_005224_04

euxassay_005224_05	euxassay_005224_06	euxassay_005224_07	euxassay_005224_08	euxassay_005224_09

euxassay_005224_10	euxassay_005224_11	euxassay_005224_12	euxassay_005224_13	euxassay_005224_14

euxassay_005224_15	euxassay_005224_16	euxassay_005224_17	euxassay_005224_18	euxassay_005224_19

euxassay_005224_20	euxassay_005224_21	euxassay_005224_22	euxassay_005224_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7417_wholemount_strong.wlz

7417_wholemount_moderate.wlz

7417_wholemount_weak.wlz

7417_wholemount_possible.wlz

7417_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7417_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7638
Entity Detected:	Lrrc8e, leucine rich repeat containing 8 family, member E ( MGI:1919517)
Sequence:	sense strand is shown >T7638 CTGATAGCGTCTGTAGTGAGGCGGGGCCGAACAATCCCGGGGGCGGGGCGGGGCGGCTCCTGGCCCAGCC CGCCCTGACTCACCCTCTCTGGCCCGCCCGGGAACTCTGCGGGAGTCCGGGTCGATCTACTCCCAGGCAT CTAGAAGTTGGGCCCCGGAGTTCCATCCCTGAGCAGTGAGCAGGATGATCCCAGTGGCGGAGTTCAAGCA GTTCACAGAGCAGCAGCCTGCATTCAAGGTGCTCAAACCCTGGTGGGATGTTCTGGCTGAGTACCTCACC ATGGCTATGCTCATGATCGGGGTATTCGGGTGCACCCTCCAGGTGACACAGGACAAGATCATCTGCTTGC CTAGCCACGAATCTCGTGAGAACATATCGGGGGCTCCATGCCAGCAGCTTTTGCCTCAAGGAATCTCTGA GCAGATGGGGGGTCTCCGAGAGCTCAGTGGCCTCAAAAACAACCTAGACCTCCAGCAGTATAGCTTTATC AACCAGCTCTGCTATGAGACTGCCCTCCACTGGTATGCCAAATACTTCCCCTACTTGGTGGTTATCCATA CTCTCATCTTTATGGTCTGTACCAGCTTTTGGTTCAAATTCCCTGGTACCAGCTCCAAAATAGAACACTT CATCTCCATCCTGGGCAAGTGCTTTGATTCTCCATGGACCACTCGGGCCTTGTCCGAGGTGTCTGGAGAA AATCACAAGGGCCCAGCTTCTGGACGGGCCATGGTGACCACGGTAACCACCACAGGGGCAGGGTCGGGGA AGGTGGGTGAAGGTGAGAAGGAGAAAGTGCTCATTGAGCCCGAAAAGGTGGTGTCTGAGCCTCCAGTTGT TACTCTGCTGGATAAGAAGGAGGGCGAGCAAGCGAAGGCCCTATTCGAGAAGGTCAAAAAGTTCCGAGTG CACGTGGAAGAGGGTGACATCCTCTATTCCATGTACATCCGGCAGACAGTGCTCAAAGTGTGTAAGTTTT TTGCCATCCTGGTTTACAACCTGATCTATGTGGAGAAGATCAGCTTTCTGGTGGCCTGTAGGGTGGAGAC ATCAGAGATCACGGGGTATGCCAGTTTCTGCTGCAACCACACCAAGGCCCACCTCTTTTCTAAGCTGGCC TTCTGCTATATTTCCTTTGTGTGTGTCTATGGCATCACCTGCCTGTATACACTCTACTGGCTCTTCCATA GGCCCCTAAAAGAGTACTCCTTCCGTTCTGTGCGTGAGGAGACAGGGATGAACGATATCCCGGATGTTAA GAATGACTTTGCCTTCATGCTCCACCTCATTGACCAGTACGACTCCCTCTATTCCAAACGCTTTGCAGTC TTCCTCTCTGAAGTCAGTGAGAGCCGCCTTAAGCAACTCAACCTCAACCATGAGTGGACACCTGAGAAGC TGAGGCAGAAGCTGCAGCGTAACATGCGTGGCCGGCTGGAGCTGAGCCTCTGCATGCTCCCTGGACTACC TGACACCGTCTTTGAGCTCAGCGAGGTGGAGGCACTCAGGCTGGAGGCCATCTGTGACATCTCCTTTCCC CCTGGGCTCTCCCAGCTTGTGAACCTTCAGGAGCTCAGCTTGCTTCACTCACCAGCCAGGCTGCCCTTTT CATCACAGATCTTCCTGCGAGATCGCCTGAAGGTGATCTGTGTTAAATTCGAGGAGCTCCGAGAGGTACC CCTCTGGGTGTTTGGGCTTCGAGGCTTAGAAGAACTGCACCTGGAAGGACTCTTTCCCCCAGAAATGGCT CGGGGTGCCACCCTGGAGAGCCTCCGGGAGCTTAAACAGCTCAAGGTACTGTCTCTACGTAGCAATGCAG GGAAGGTGCCAGCCAGTGTGACTGATGTGGCCGGGCACCTTCAGCGACTCAGCCTGCACAATGATGGGGC TCGTTTGCTTGCCCTAAACAGCCTCAAGAAGCTGGCAGTGTTACGGGAGCTGGAGCTGGTGGCCTGTGGG CTGGAACGTATCCCCCATGCAATCTTCAGCCTGGGGGCTCTGCAGGAACTTGACCTTAAGGACAACCACC TTCGGTCTATCGAGGAGATCCTCAGCTTCCAGCACTGTCGGAAGCTGGTCACTCTCAGGCTGTGGCACAA CCAGATTGCCTATGTGCCCGAGCATGTGAGAAAGCTCCGGAGCCTGGAGCAGCTCTACCTCAGTCACAAC AAGCTGGAGACTCTGCCCACCCAGCTGGGCCAGTGCTTTGGCCTCCGCCTGCTGGATTTATCCCACAACG GGCTTCGTTCCCTGCCACCTGAGTTGGGCCTTCTCCAGAGCCTCCAGCATCTAGCCCTCTCCTACAACGC ACTCGAGAGTCTGCCCGATGAATTGTTCTTCTGCCACAAGCTGCGGACGTTACTCTTGGGCTACGACCAC CTAACTCAGCTCTCACCTGACGTGGCTGCCCTCCAGGCCCTCAGCCGTCTGGGGCTCAAGGGTAACAGGT TGGAGACACTGCCAGAAGAACTTGGTGACTGCAAGGGGCTGAAGAAGTCGGGGCTGCTGGCGGAGGACAC CCTTTATGAGGGACTGCCTGCAGAAGTTCGGGAAAAGATGGAGGAGGAATGAAGCCTGGTGGGGGAAGGG GCAGGCTGACACAGAGGCCTTTACATGTTTCTGCCCAACATTGTATTCCAAGGGAGAGAGGTCCAGCAGG AGTGGAAGGAAGGCAGGACATGTCGGTTTTGTGCAGCTCAGACAAGATCCTGGGACTGGTTTTTCTGGAG GAGAAACAGGAGGCGGTAGATTTGAGGTGTAACCCCTTAGGGAAGGATTAACCAGTCTTAGGTTCTTCAA CCAAAACCACACCTGTGTCTTTGGCTGGCTGGCTTGCCCTTCCTCTGAACATTCTGGCTTAATTAGTTTC CTATATGGGAGTGGGATTTCCAACCTTTCCCAACAAGGATCAGCATATACTTAGGGTTCTCTCCTAAGGA GAGGATACAGGGTCCAGTTCCAAAGTTCCATATCCCAACTTTCATGCAAGTCCTGATTTTATAAGCCCTT TGTGACAGGGATGGGTACTTAGAACAATGGCTCTCACATTTAGCTATGTAATAAAATCAGCTGAGAGGCC CGGAAGAAAACAAAACCCTTACATGCTTCCCACCCCACCCCTGAAATTATACTGACTCAGAATTTGGATG GGTAGCTGAGCATGGTGGCACACGCCTTTAATTCCAGCACTCAGGAGGCAGAGGCAGGTGGATTTCTGAG TTCGAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30481669 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30481669 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7413 same embryo
	EMAGE:7414 same embryo
	EMAGE:7416 same embryo
	EMAGE:7412 same embryo
	EMAGE:7415 same embryo
	EurExpress:euxassay_005224 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source