EMAGE mouse gene expression spatial database

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EMAGE:7540

Mef2b myocyte enhancer factor 2B ( MGI:104526)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004647. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004647_01	euxassay_004647_02	euxassay_004647_03	euxassay_004647_04

euxassay_004647_05	euxassay_004647_06	euxassay_004647_07	euxassay_004647_08	euxassay_004647_09

euxassay_004647_10	euxassay_004647_11	euxassay_004647_12	euxassay_004647_13	euxassay_004647_14

euxassay_004647_15	euxassay_004647_16	euxassay_004647_17	euxassay_004647_18	euxassay_004647_19

euxassay_004647_20	euxassay_004647_21	euxassay_004647_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7540_wholemount_strong.wlz

7540_wholemount_moderate.wlz

7540_wholemount_weak.wlz

7540_wholemount_possible.wlz

7540_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7540_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

single cell

strong expression: see section 08 09 11 12 13 14 15 moderate expression: see section 06 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7704
Entity Detected:	Mef2b, myocyte enhancer factor 2B ( MGI:104526)
Sequence:	sense strand is shown >T7704 GGCGCCTGGACCCTCACAGTGCGCAGCAGGTGTGGCCAGCAGGGACCAGACTCCCGGGACTCCTGAGCCG CTCTGCACAGCTACCCACGATGGGGAGGAAAAAAATCCAGATCTCACGAATTCTAGACCAAAGGAACAGG CAGGTGACTTTCACCAAGCGCAAGTTCGGACTGATGAAGAAGGCTTATGAGCTGAGCGTGCTTTGTGACT GCGACATTGCCCTGATCATCTTCAACAGCGCGCAACGCCTCTTCCAGTATGCGAGCAGCGACATGGACCG GGTGCTGCTCAAATACACCGAGTACAGTGAGCCGCACGAGAGCCGCACCAACGCGGATATCCTTCAGACA CTGAAGAGGAGGGGCGTGGGCCTTGATGGGCCCGAGCTGGATATGGAGGAGGGGCCAGAAGGTCCTGGAG AGAAGCTGCTGAGGACACTGGGGGGTGACAGAGGCTCGGCCTCGCCCCCGCCTCGGATCTATCCCGTGGC CCCCGCAATGTCGGTTTCAGAGCTGTCCTACCGGGTCCCACCAGCCACTCCAGGCTGTGATCCTGGCGGG CTGGGGGAGGCCCCGTCTGTCCACAGCCGCCCGCCTCACTTTCGACCCCCGGGCCTGGGACACCCGATCT TCTCTCCAAGCCACCTTGCGAGCAAGACGCCCCCGCCTCTGTACCTGGCGACTGATGGGCGGAGGCCAGA CCTGCCCCCTGGCCTGAGAAGCCTGTACAGCGGCCTGCAGAGTCCCGGTGCCCCAGGCCCCGCTCTGGGT AGCTTTGCCTTCTTACCATCAGGCTCCACAGATTGCAGCCCGGGAGACGCAGCTCAGGGACCCCTGCAAC CCTCACCCTGGCCACCAACGAGGGACGCTGTAGACCCCGCCCGGCCAGTTGCCCGCAGTCTGTGCAAGGA GGGTCCCCCCAGCCGAGGGGCCTCCCCTCCGACACCTCCGGTCAGCATCAAGTCTGAGCGACTGTCTCCA GTCACTGGGACCTCTGGCGACTTCCCCAGGTCCTTCCCCTACCCGCTGCTCCTTGCCAGGCCCCTTGCAG AGCCACTGCGGCCCTCGGCCTCCCTGCACCGCTTGACCCCTGACAGCTGGCCACGGTAGCGCTCCAAGAG GTTCCTCCAGCCTCCACAGAGTTCCCGAAGTCTCAGGTTCCCTATACCACCAGGACGGACGGACGAGGGC ACCCACTGTGCATCACTAAGGCCCTGTCCCTTCCTGGGGGCCGCTGCATCAATAAAGCCCAAGGACTCGG GAGGGCACTGGGCAGCCCCTAGCTCAGAGCTCCCGAGCCTGGAATAGCCACAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6307224 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6307224 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7535 same embryo
	EMAGE:7537 same embryo
	EMAGE:7536 same embryo
	EMAGE:7539 same embryo
	EMAGE:7538 same embryo
	EurExpress:euxassay_004647 same experiment
	MGI:4826154 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source