EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7595

Ipo5 importin 5 ( MGI:1917822)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004702. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004702_01	euxassay_004702_02	euxassay_004702_03	euxassay_004702_04

euxassay_004702_05	euxassay_004702_06	euxassay_004702_07	euxassay_004702_08	euxassay_004702_09

euxassay_004702_10	euxassay_004702_11	euxassay_004702_12	euxassay_004702_13	euxassay_004702_14

euxassay_004702_15	euxassay_004702_16	euxassay_004702_17	euxassay_004702_18	euxassay_004702_19

euxassay_004702_20	euxassay_004702_21	euxassay_004702_22	euxassay_004702_23	euxassay_004702_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7595_wholemount_strong.wlz

7595_wholemount_moderate.wlz

7595_wholemount_weak.wlz

7595_wholemount_possible.wlz

7595_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7595_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8632
Entity Detected:	Ipo5, importin 5 ( MGI:1917822)
Sequence:	sense strand is shown >T8632 GCAATGGCGGCGGCCGCGGCGGAGCAGCAACAGTTCTACCTGCTCCTGGGAAACCTGCTCAGCCCCGACA ATGTGGTCCGGAAACAGGCGGAGGAAACCTATGAGAATATCCCAGGCCGGTCCAAGATCACATTCCTCTT ACAAGCCATCAGAAACACAACCGCTGCCGAGGAGGCTAGACAAATGGCTGCTGTTCTCCTAAGACGTCTT TTGTCCTCTGCATTTGATGAAGTCTACCCAGCTCTTCCATCAGATGTCCAGACTGCCATCAAGAGTGAAT TGCTAATGATCATTCAGATGGAAACACAATCCAGCATGAGGAAGAAAATCTGTGATATTGCTGCAGAATT GGCCAGGAATTTAATAGATGAGGACGGCAACAACCAGTGGCCTGAAGGTTTGAAGTTCCTCTTTGATTCA GTCAGCTCACAAAACATGGGACTCCGGGAAGCTGCCCTTCACATATTTTGGAACTTTCCTGGAATTTTTG GGAACCAACAACAGCACTATTTGGACGTCATCAAACGGATGTTAGTTCAGTGTATGCAAGATCAGGAGCA TCCTTCGATCAGGACACTGTCTGCCAGAGCTACTGCTGCCTTCATACTTGCCAATGAGCATAATGTCGCT CTGTTCAAACACTTTGCAGACTTGCTGCCTGGATTCTTACAGGCTGTCAATGACTCATGCTACCAGAATG ATGACTCGGTCCTAAAATCCCTTGTTGAGATTGCAGACACCGTACCAAAGTACTTGCGCCCTCACTTAGA AGCAACTCTGCAGTTGAGTCTGAAGTTGTGTGGAGACACTAACCTCAATAATATGCAGCGCCAGCTTGCC CTTGAAGTGATTGTGACACTGTCAGAGACTGCAGCTGCTATGTTAAGAAAGCATACCAGTCTCATCGCAC AGACGATTCCTCAGATGTTGGCAATGATGGTTGACCTAGAAGAAGATGAGGACTGGGCAAATGCTGATGA GCTAGAAGATGATGATTTCGATAGCAATGCAGTTGCTGGTGAGAGTGCATTAGACCGAATGGCCTGTGGA CTTGGTGGAAAGCTCGTTCTGCCCATGATTAAGGAACACATTATGCAAATGCTTCAAAACCCTGATTGGA AATACAGGCATGCAGGACTGATGGCCCTATCCGCCATTGGCGAAGGATGCCACCAGCAGATGGAAGGAAT TCTAAATGAGATTGTAAATTTTGTCTTGCTTTTCCTCCAGGATCCTCATCCGAGAGTCCGGTATGCAGCC TGCAATGCTGTGGGTCAAATGGCTACAGATTTTGCACCTGGTTTTCAAAAGAAATTTCATGAGAAGGTGA TTGCAGCTTTGCTTCAGACCATGGAAGACCAAGGCAACCAGCGGGTGCAGGCCCATGCAGCGGCTGCCCT CATTAACTTCACTGAAGATTGTCCCAAGTCGCTGCTTATTCCCTACTTGGATAACTTGGTGAAGCACCTG CACTCCATTATGGTGCTCAAGCTCCAGGAGCTGATTCAGAAAGGCACCAAGTTAGTTTTGGAACAAGTTG TGACATCCATTGCATCAGTTGCAGATACTGCAGAAGAAAAGTTTGTCCCCTACTATGACTTGTTTATGCC ATCACTGAAGCACATTGTCGAGAACGCAGTCCAGAAGGAGCTGAGACTGCTGCGAGGGAAGACCATCGAG TGCATCAGCCTCATCGGGCTGGCCGTCGGGAAGGAGAAATTCATGCAGGATGCTTCAGATGTGATGCAGC TATTGTTGAAGACACAGACAGACTTCAATGATATGGAAGATGACGACCCCCAGATTTCTTACATGATCTC AGCATGGGCCAGGATGTGCAAAATCCTTGGGAAAGAATTCCAGCAGTACCTTCCCGTGGTTATGGGGCCG CTGATGAAGACTGCTTCAATTAAGCCTGAAGTGGCCCTTCTAGACACCCAGGACATGGAGAATATGAGTG ATGATGACGGTTGGGAATTTGTGAACCTCGGAGATCAGCAAAGTTTTGGTATTAAAACTGCAGGACTGGA AGAAAAGTCAACGGCTTGTCAGATGTTGGTTTGCTATGCTAAGGAATTAAAGGAAGGTTTTGTGGAATAC ACTGAACAGGTTGTCAAACTGATGGTCCCGCTGCTGAAATTTTATTTCCACGATGGGGTCCGAGTGGCAG CAGCAGAGTCCATGCCTCTTCTGCTTGAGTGTGCGAGAGTCCGGGGTCCCGAGTACCTTACACAGATGTG GCACTTCATGTGCGACGCTCTCATCAAGGCCATCGGCACAGAGCCCGACTCAGATGTCCTCTCGGAGATC ATGCATTCCTTTGCAAAGTGCATTGAGGTCATGGGAGACGGGTGCCTCAACAATGAGCACTTTGAGGAGC TGGGAGGCATCCTGAAGGCGAAGCTTGAAGAACATTTCAAAAATCAGGAGTTGCGGCAAGTTAAAAGACA AGATGAAGACTATGACGAGCAGGTTGAAGAGTCACTACAAGATGAGGATGATAATGATGTTTATATACTG ACTAAAGTGTCGGATATTTTACACTCAATATTCAGTAGCTACAAAGAAAAGGTGTTGCCGTGGTTTGAAC AGCTGCTCCCATTAATTGTCAACCTGATTTGTCCGCAAAGACCATGGCCAGACAGACAATGGGGATTGTG CATCTTCGATGATATCGTAGAACACTGTAGTCCAGCTTCATTTAAGTATGCAGAATATTTCATAAGTCCA ATGCTCCAGTATGTGTGTGACAACAGCCCAGAAGTCAGGCAAGCTGCGGCATATGGCCTCGGCGTCATGG CGCAGTTTGGTGGAGATAACTACCGCCCTTTCTGTACCGATGCACTCCCGCTGCTTGTAAGAGTCATTCA GGCTCCAGAGGCTAAGACCAAAGAAAATGTCAACGCCACGGAGAACTGCATCTCAGCAGTGGGAAAGATC ATGAAGTTCAAGCCCGACTGTGTGAATGTGGAGGAGGTCTTACCACACTGGCTGTCATGGCTGCCCCTAC ACGAAGATAAAGAAGAAGCTGTCCAGACCTTCAGTTACCTGTGCGACCTGATTGAAAGTAATCACCCAAT TGTTCTTGGCCCAAACAATACCAATCTACCGAAAATATTCAGTATAATTGCAGAAGGAGAAATGCATGAG GCCATTAAACATGAAGACCCCTGTGCCAAACGCCTGGCCAATGTAGTTCGCCAAGTACAGACTTCTGGAG GATTGTGGACTGAATGCATAGCTCAACTCAGTCCGGAGCAGCAGGCAGCCATCCAGGAGCTCCTGAACTC TGCCTGAAGGGCCTTAACTACCACCTGCCAGAAGACTAACTCCAAATAAACGTTTACCCTTTTGTTTAGG TTTCTTTGTTTTGTTTTTGAGCAAGAGAGCAGTAGTGTTGTGTGTAGGCCGTTCTTCTGGAGCCGTGCAC AGGAAAAGTAACCCTGTGTGCAGGGTGGACTGCCATGGATCTCATCAAACCCGCAGAGGAGGTCCCTGTA AGCCTGATGGTAGCACTGCAGAGTATTTTTCTATTGCTGTGTCTGACCCACAAGAAGGGAAAGAGGAAAC GATTCCTTTTTTCTCATAGACCTGCAGAAACGTAAGGAACGCAGCTTTCAGATCAGTTCATCCACATAGA TGTGTGTTACACAGACTGGGGGACAACCTAGTGTTTATTCTGTAAATGGAGTGAGAGTCTGGGGGGATGG AAAGAAGCCAACCAGCTGCTGACAAGCCAGAAGCAAAGGGCTGTCACTTGATGTCACGCTGGTTCTTTGC AAGAAGGCTCATGCAGTGACTCGGTGTGGAGGCTGTCCCACTCCCTCCCGGCCCTTGAATGTGAAGTGTC GTTGGCATGTCTGGCAGTCGTCTCATTCACTCCTCAATAAACAGCATTGAAATACAAAGAGGCCTGTGTA GAACTTCAGTACTGTCTGTCGTTGATTCCTTTCATGTTTTAATAGAAGAGTGATCTGATATTTTTTTAAG GTGATAGTTCTGGCTCAGTGTTTTACCTCCACGCTCTGCAGTGCAGCCTTGGAAGTGTGTCTCCTGTCTT GGAAAGCGCAGTTATAACACAGCTACTCTGTTCACAATTGGTATTCTGAAGGTTTGCACATTCAACCGGC TTTTTTAAAGCTTAGGGTTGTTCTGTAGATCCAAACGTTTTGGCTTAGGATTTCCATTGCAGATTGTAAT TGCTTTAGAGACACACGCAGCTTTTGAATTTTTTTAATTTTACAAGTATTATGGCTAAACTTATTTACTG AAAATTTCAGTAAAATGTGTGAAATTTTCTTTATGTATTAACCTCAGAGCAGTAACTGATGAGCTGTTGT TTCCTTTAAGTAATTTTCTAAGGGATCTTAATAGATTTCTGTTGAAACCTTGGTGTTAACGTTTTCATAG TTTGTACTAAATTTAACTGTGATACAAAAATGAGTTTTATGCATAAATCAGAATTTAAAATTAAAAAATT TTTTCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5719784 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5719784 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7598 same embryo
	EMAGE:7599 same embryo
	EMAGE:7597 same embryo
	EMAGE:7600 same embryo
	EMAGE:7596 same embryo
	EurExpress:euxassay_004702 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source