EMAGE mouse gene expression spatial database

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EMAGE:7598

5730590G19Rik RIKEN cDNA 5730590G19 gene ( MGI:1924261)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004687. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004687_01	euxassay_004687_02	euxassay_004687_03	euxassay_004687_04

euxassay_004687_05	euxassay_004687_06	euxassay_004687_07	euxassay_004687_08	euxassay_004687_09

euxassay_004687_10	euxassay_004687_11	euxassay_004687_12	euxassay_004687_13	euxassay_004687_14

euxassay_004687_15	euxassay_004687_16	euxassay_004687_17	euxassay_004687_18	euxassay_004687_19

euxassay_004687_20	euxassay_004687_21	euxassay_004687_22	euxassay_004687_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7598_wholemount_strong.wlz

7598_wholemount_moderate.wlz

7598_wholemount_weak.wlz

7598_wholemount_possible.wlz

7598_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7598_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

weak

regional

weak expression: see section 14

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 11 15 16

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 01 02 03 21 22

midbrain ventricular layer

strong

regional

strong expression: see section 13 14 moderate expression: see section 07 08 09 10 11 12 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8678
Entity Detected:	5730590G19Rik, RIKEN cDNA 5730590G19 gene ( MGI:1924261)
Sequence:	sense strand is shown >T8678 GAGATGCAAGCTTCTGGCCTTCCCAAGCTCCGGATTAAAAAAATAGACCCTGGAGTTCTTTTGGAAGCTG AGGCCCTAGGAAAGGAAGCCCCCCTGGGAGAGGAGGGTGCCCTCCCTGCTCTCTGTATGCCCAAGGCCAG CAAGTCCTCCGGGAGAACTGAGCACCCTTACTTGTCACCCCCTTGTCTCCGCCCCTCACACAGCACACCT GGCAAGAATGGAGGTCAAACCTTCATCTGCCAATCTTGTACTCCCTCTCGCTGTCCCCCCAGTACCCCCT CTCCATTTCAAGCAGATGCTGGGGTTTCTTGGACACCATCCCCTAAGCAAAGTGGGAAGACCACTCCTGA AATTATCAAAGACTGGCCCCGCAGGAAGAGGGCAGTGGATTGCAGTGCTGGACCCTCTGCTGGGAGAGGC GAGGCCAGTATGGACCTTCCTGGGTCCTTGTCACTGCTTGAGCCTGAGCCTGAGGGAAAGGAGCGAAGCC TTGAACAAGACCTCTCTAAGGTTCTCATCTCAGAGGAGTTTGAACTAGAGGGGGTGTGTCAGTTGCCAGA CCAGTCACCTCCCAAAGACAGTGCCTCTGTGACAGAGGAAACCTCCTGGGGACAGTTTGGATTAGGCAGA AAGAGATTCCTGTCCGCCAAGGAAGAATCTGAATACAAAGTCAAAAGGGTCTGTGATTCCCTGAGTGAGG ATCCCCAAGCTAGCAAGCAGAAAGAGTGCTCTCCACGCTGGAGTGCACTACCACTCCACTCAGTAGGGGA CGACGAGGTGTTTGTTTCTGGCTCCACCCCACCCTCTGGTTGCATGGTTCGAAGCTGTCTCTCTGCCAGT GGTCTGCAGGCCTTGACCCAGTCTCCACTGCTGTTCCAAGGAAGAACGCCCTCCTCTCATAGCACGGACA CCAGAGATGAGGAAGTGGATGTGTTCCCCTCTACTGCTGAAGAGTCTCCGTTCAGCCACACCCTCTCCCG GAAACGGCCTTTCAGAACCTACACGAGAAAGAAGCTTATTAGCTAGCCCTGCTTGAGCATTGCGCTGGAG GACTCAAAATGAGGAACGCTGTGAGCCTGGCTAGACCCCTTGCACTCAGGAGATGTACTCTAGAGCAGCT TTCTCTGGGTACAGTAACAATGCCAGACTCATGCCAGCAATCTAGCATTGTGCCCAGAACACGAGTGGCC AGGACTTCATGGGACTGAGAAAGTTCACAAGTAAAGATGACAGCAGCAAAGTCCCTGTGGACTTAAAGAA ATTGAAGATTCTGCAGGGTATGGTAGTAAGCCTATAGTCCTAGCACTTGGGAGACGGAGACAGAAGATTC TGACTACATAGCATGTACAAGGGCAGCCTGGGCTACATGAGACCCTGTCTCAAAAACAACAAAAGACATT AAAGACCTTAAGCCTTTTTCCCATGGTCATCTGAAGATTACCACTGGAATGCCTCATGGTCTGAAAATTC CTCTCTAGGTCTTCATATGAGGCCAGGCCCTGACTCTTTAGGGGGAAGGTTGGAAGTCTGGTGGGCAGCT AGTACTATATATAACTCAGACAGCCCAAAGATCCCATTCCAGATCTTGGTAATTCAGAGGACTACTGCCA CACTGTGAAATGGAAAAAAAGGAGGTGTTTACCAAGCCTTTTCTCTGGGTACAGTAACAATGCCAGAGTT CTTTTACACAATTGGTACTTCATAAAGTAAGACCCATAAGTGAAAGACGTCAGCAAGAAGCAAGCTAACA ATAAAGTCATCCTGGCAACTTAGCATTTGGCATTTCTGCCTGGGAGTAACTGGCTATGGTCCAGAGCAAT CATACTGCAGGGTCTACCCTGAAAAGCAGCCACCGCATGACAGTCTTAGCCAGGTATGTGCTTGACACCT GTGTTGCCCTATAATCAACCTAACCAACTTGCTATGGAGTGTTGGCAGGAGCCTCATTTGCAGTGAGGGC TGATTTGGAACCTTCTCAGTCTCCCCAGAAGATTCAACCCTGTTGTGGTCCCAGGGCTCTTTCTGTTGTC AGATGCCAGGTGAAAACTCCCATGTCTCCTTTCAGAGAAAGAAAAGGCAGCCCTGGAATGCTTGCATGGG TTCAATTGCCAGTAACACTGATGAGTAGAGAAGGAAGGGTTTCAAGCTGGCTCTAACTAAGTTCATAGTT ATTTGTCCTGAGTTGGGAGCCTGTGCACAGGCAGTAGGAGGGGAGAGGCAGGACTCCAATATGCCCTTCA AGGATACTCTCCAATAACCTCACCACCACCACACCCCAGCTCTGATAGGCTCCACTGCCTCTCAGCTGAG GACCAGCCTTCAGGAATTCTTGGGGGACATATTTAAGATGTAAGCCATAATGAGATTACTCCTGTTTATT ATTAAATGGTATTTTTTAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6400795 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6400795 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7599 same embryo
	EMAGE:7597 same embryo
	EMAGE:7600 same embryo
	EMAGE:7595 same embryo
	EMAGE:7596 same embryo
	EurExpress:euxassay_004687 same experiment
	MGI:4822770 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source