EMAGE mouse gene expression spatial database

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EMAGE:7601

Gtpbp2 GTP binding protein 2 ( MGI:1860138)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004692. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004692_01	euxassay_004692_02	euxassay_004692_03	euxassay_004692_04

euxassay_004692_05	euxassay_004692_06	euxassay_004692_07	euxassay_004692_08	euxassay_004692_09

euxassay_004692_10	euxassay_004692_11	euxassay_004692_12	euxassay_004692_13	euxassay_004692_14

euxassay_004692_15	euxassay_004692_16	euxassay_004692_17	euxassay_004692_18	euxassay_004692_19

euxassay_004692_20	euxassay_004692_21	euxassay_004692_22	euxassay_004692_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7601_wholemount_strong.wlz

7601_wholemount_moderate.wlz

7601_wholemount_weak.wlz

7601_wholemount_possible.wlz

7601_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7601_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8642
Entity Detected:	Gtpbp2, GTP binding protein 2 ( MGI:1860138)
Sequence:	sense strand is shown >T8642 GGCGGTGGCAGTAGGGGCGGCGGCAGTGGCGGGGGGGAGGGGTCGGGCGGGGGGCTGGTGGCAGCGGCGG ATGGACTCGCGGGTATCGGAGCTGTTCGGCGGCTGCTGCCGGCCCGGAGGAGGCCCGGCCATGGGTGGAA ACCTCAAAGCTCGGGGGGCCGGCGGTAGCAGCAGTTGCGGGGGCCCAAAGGGGAAGAAGAAGAACGGAAG GAACAGAGGAGGTAAAGCCAACAACCCTCCGTACCTGCCCCCAGAGGCTGAAGATGGAAACATCGAATAT AAACTGAAGCTGGTGAACCCATCTCAGTACCGCTTTGAGCACTTGGTGACACAAATGAAGTGGCGGCTCC AGGAAGGACGTGGTGAGGCTGTCTACCAGATTGGGGTAGAGGACAATGGGCTGCTGGTGGGGCTGGCTGA AGAGGAGATGCGGGCTTCCCTCAAGACCCTGCACCGGATGGCAGAGAAGGTTGGGGCAGACATCACTGTT CTTCGGGAGCGAGAGGTGGATTATGACAGTGACGTGCCCCGGAAGATCACTGAGGTGCTAGTACGAAAGG TCCCTGACAACCAACAGTTCCTAGATCTCCGTGTGGCCGTCCTAGGGAATGTGGACTCAGGGAAGTCAAC CTTGCTTGGAGTCCTGACCCAAGGAGAGCTGGACAACGGGCGGGGCCGGGCCCGGCTCAACCTTTTCCGC CACCTGCATGAGATTCAGTCTGGCCGAACCTCCAGCATCAGCTTTGAGATCCTGGGCTTTAACAGCAAGG GAGAGGTGGTGAATTACAGCGACTCACGGACTGCAGAAGAGATCTGTGAGAGCAGCTCCAAGATGATCAC CTTCATTGACCTGGCGGGCCACCATAAATACCTGCACACCACCATCTTTGGCCTCACCTCCTACTGCCCC GACTGTGCCCTGCTCCTCGTCAGTGCCAACACTGGAATCGCCGGCACCACACGGGAACACCTGGGGCTGG CCTTGGCCCTGAAAGTGCCCTTCTTCATCGTGGTCAGTAAAGTGGACCTGTGTGCTAAGACCACAGTGGA GAGGACAGTTCGCCAGCTGGAGCGGGTCCTCAAGCAGCCTGGCTGCCACAAGGTCCCCATGCTGGTCACC TCTGAGGATGATGCTGTCACTGCTGCCCAGCAGTTTGCCCAGTCACCCAATGTCACCCCTATATTTACAC TGTCCAGTGTGTCTGGAGAGAGCCTGGACCTTCTTAAAGTCTTCCTGAATATCCTGCCGCCCCTCACCAA CAGCAAGGAGCAGGAGGAGCTCATGCAACAGTTGACAGAGTTCCAGGTGGATGAAATCTACACAGTCCCA GAGGTGGGGACTGTGGTTGGAGGAACACTATCCAGTGGGATCTGCCGCGAGGGGGACCAGCTGGTGGTAG GCCCGACAGATGATGGCTGTTTCTTGGAGCTGAGGGTATGCAGCATCCAGCGCAACCGCTCTGCGTGTCG TGTTCTTCGAGCAGGTCAGGCTGCTACACTCGCCCTTGGAGACTTTGACCGTGCATTGCTTCGCAAGGGC ATGGTCATGGTGAGTCCCGAGATGAATCCCACCATCTGCTCAGTGTTTGAGGCAGAGATAGTCCTACTGT TCCACGCCACCACCTTTCGGCGGGGATTCCAGGTGACAGTACATGTGGGCAACGTACGTCAAACAGCAGT GGTGGAAAAGATCCACGCAAAGGACAAGCTGCGGACAGGGGAGAAGGCAGTGGTGCGTTTCCGTTTCCTG AAACACCCAGAATACCTGAAGGTGGGCGCCAAACTGCTGTTCCGGGAGGGTGTGACCAAAGGCATCGGTC ATGTCACGGATGTACAAGCCATCACAGCAGGAGAGGCCCAGGCCACCATGGGCTTCTGAGTCATCCAAAC AGCTCTACTGCTGTCCTACAATACATGAGGTGACTTCCGGCCATGCTGCCCACTGTTGGCGGCTCTGTGT GTTAATAGGCTAGGGAGAGGAGTGTCTGCTGCCGCTTGCTTCCTGCCACGTTCTTGGAGAGGTGTCAAGT TTGGTATGGCCAGGGAGGGGCAGTCCTGAGGGAGAAGACGGTTCAGGGCAGTGCTCAGCAGCTGTGTGCT CCCCTGGTTGGGTCATTAACCCTGCAGCTGCCTGGTCTTGTCTGCTGGAGGGAGGAGAACACAGCCATCT TGGCCCACTATCAGGACCAGGCATGACTCACCAGTGTCATCTCTGCACTTCAGGAATTGTCAACAACTGA GGTGGCCCTTGTCAGCACTTCTTTTTTTATACCTCCTCTCAAGGCCACGTAAGTTACCTAGCTCTACCTG ACTGTGGATAAAAGACAGTGCTCTACCCACCTGGTGGACCCAGGGACTGAAGCAATGGCACAGAGACCAT GACAGACTGTGCTCCTATCCTGGGTTGAGTCCCGATGCATATTCCTGCTTCATTTCCTTTTCTCAAGGGC CGCTTCCCCGGACCCTCCTGTCATAGGCCCCACTTGGTGCTATTTGTGGTGCTGGCTCAGGCGTGGGGCT GCCAGGCTCGGGCTTGGCACACCAAAGGCCAGCTGCATGTCAGTTTCTCTCCTTCCACAGTTATGTTGGC TTCCATGCTTTTTCAGATTGTGTGGTAGCCATAGGACCTGACCCAGTTTGTGCCTCCTGGGATCCTCATC CCCAATCCTGCTCTTACATAAACCTTTTTCTTGTTTTAGTAATTTGTAGTGATCACCCCCTTCCCTCTGT TGCTGGGACCCAGGAGGAAAGGGGAAGATGTCCTGTTTCCTATCCTAGGTGTGGACGCCCGCCTTGCCCT TTGTTAGCTTCCTGGGTTCCCATGAACTCAGGGATTTGTTGGAAGATTCTCTGCATAATAGATATATATG TATATATATTTAAATACACATATATATTGTACAGAATAAAAAATGTTTTATTGGAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:5720710 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5720710 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7604 same embryo
	EMAGE:7606 same embryo
	EMAGE:7603 same embryo
	EMAGE:7605 same embryo
	EMAGE:7602 same embryo
	EurExpress:euxassay_004692 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source