EMAGE mouse gene expression spatial database

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EMAGE:7637

Nkx2-1 NK2 homeobox 1 ( MGI:108067)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004735. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004735_01	euxassay_004735_02	euxassay_004735_03	euxassay_004735_04

euxassay_004735_05	euxassay_004735_06	euxassay_004735_07	euxassay_004735_08	euxassay_004735_09

euxassay_004735_10	euxassay_004735_11	euxassay_004735_12	euxassay_004735_13	euxassay_004735_14

euxassay_004735_15	euxassay_004735_16	euxassay_004735_17	euxassay_004735_18	euxassay_004735_19

euxassay_004735_20	euxassay_004735_21	euxassay_004735_22	euxassay_004735_23	euxassay_004735_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7637_wholemount_strong.wlz

7637_wholemount_moderate.wlz

7637_wholemount_weak.wlz

7637_wholemount_possible.wlz

7637_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7637_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thyroid gland

strong

regional

strong expression: see section 10 11 12 13 14 15

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

telencephalon mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

trachea

moderate

regional

moderate expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8906
Entity Detected:	Nkx2-1, NK2 homeobox 1 ( MGI:108067)
Sequence:	sense strand is shown >T8906 CTGGTAACAGCAATGAGGCTGACGCCCCCGGGCCCGCTAGGGAGCACAGCCCACAGCTCCCCCTTGCCAG GCGCCCAAGGACCCTCAAGGCGCGGGGCTCACACTTGAAGCCTGGGAACGCTCAGACAGGAAACCCACTT CCTCCTAAGCAGTTTCTTCCTAGCCGGATGAGAGGCGCCCAATTGAAGCAGAATGATCCTCATCTACTAA TATCCAGCGTGGCCACAAAGCGACCGGCCATTTACGCCGCCACTTTAGACAAAGATATTTGGTTATTCCC GGGGAAGCAAGTGCACTTTTGCATGGCTGAGCTCCGGGAGGAGGCGAGCCTCAGCCCAGCCTCCCGCCCG CTGGGCTGCGGGCGTCGAGATATTCGCCTCCTCCCGGACAACGAGTTCCACCCGGGTTCAGACTCAGTTC CACTCTGCAACGGATCTGCGGGCGCTCACGCGGCTCCCCGCCCGGGCTTTCACTGAAGCATCGGAAGGGA AAACTGCGGGGATCTGAGCTGGGGTGCTGGGACTGGGATGTCCTCGGAAAGACAGCATCAGCTTCTGAAG CCGAAGTATCCAGGCCATGGGCAAGGGTCAGGGGCACCAGCCGACGCCGAATCATGTCGATGAGTCCAAA GCACACGACTCCGTTCTCAGTGTCTGACATCTTGAGTCCCCTGGAGGAAAGCTACAAGAAAGTGGGCATG GAGGGCGGCGGCCTCGGGGCTCCGCTCGCAGCGTACAGACAGGGCCAGGCGGCCCCACCGGCCGCGGCCA TGCAGCAGCACGCCGTGGGGCACCACGGCGCCGTCACCGCCGCCTACCACATGACGGCGGCGGGGGTGCC CCAGCTCTCGCACTCCGCCGTGGGGGGCTACTGCAACGGCAACCTGGGCAACATGAGCGAGCTGCCGCCT TACCAGGACACCATGCGGAACAGCGCTTCGGGCCCCGGATGGTACGGCGCCAACCCAGACCCGCGCTTCC CCGCCATCTCCCGCTTCATGGGCCCGGCGAGCGGCATGAATATGAGTGGCATGGGCGGCCTGGGCTCGCT GGGGGACGTGAGCAAGAACATGGCCCCGCTGCCCAGTGCGCCCCGCCGGAAGCGCCGGGTGCTCTTCTCC CAGGCGCAGGTGTACGAGCTCGAGCGACGTTTCAAGCAACAGAAGTACCTGTCGGCGCCGGAGCGCGAGC ATCTGGCCAGCATGATTCACCTGACACCCACGCAGGTCAAGATCTGGTTCCAGAACCACCGCTACAAGAT GAAGCGCCAGGCTAAGGACAAGGCGGCGCAGCAACAACTGCAGCAGGACAGCGGCGGCGGCGGAGGCGGC GGTGGCGGTGCGGGATGCCCGCAGCAGCAGCAAGCTCAGCAGCAGTCGCCGCGCCGGGTGGCCGTGCCGG TCCTAGTCAAAGACGGCAAACCCTGCCAGGCGGGCGCCCCTGCCCCGGGAGCCGCAAGCCTGCAAAGCCA CGCGCAGCAACAAGCTCAGCAGCAGGCGCAGGCGGCGCAAGCGGCTGCCGCGGCCATCTCAGTGGGCAGC GGTGGCGCGGTTCTAGGAGCACACCCAGGCCACCAGCCGGGCAGCGCAGGGCAGTCCCCGGACCTGGCGC ACCACGCAGCCAGCCCCGCGGGGCTGCAGGGCCAGGTCTCCAGCCTATCCCATCTGAACTCCTCGGGCTC GGACTATGGCGCCATGTCTTGTTCTACCTTGCTTTATGGTCGGACCTGGTGAGACGTGAGATGCGCTTGA GCCCCGCGCGACCTCAACGCTTCCCCTCTGCCTTCCGCAAAGACCACCATTCGCCCGCTGCTCCACGCGC TTCTACTTTTTTTAAGAATCTGTTTATGTTTAGACCAAGGAAAAGTACACAAAGACCAAACTGCTGGACG ACTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT CTTCTTCTTCTTCTTCTTCTTCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCTTCCTCCTTCTTG TCCCCGCTCGTTCTTTTCTTTCTCCCCCTCCTCTTCTGTTTCCTTCTTCCTTCATCTTTCCCCCCTTCCT TTCTCTTTACTATCTAAAACTTGCAGACTTTTTGTTTTTTAACATAAAAAGAAAATAGAAACAGCCAAGC AAATTCAACCCTTTACGGATTCTTTAAACAGAGAAGGACAGAGAACAAATTTGGGGTGTCTTTCTGGTAG TTCAAATGGGTTCCCAAGCTTAGGCATGGCACAGTTTTGGAGCCTGTTCTATGCTTCCATGGCCCTGAAC TCTAAAGACGGAAAACTTTTCTGTGGATGCACCCTGCCAGCAAAGTGAGCTTGCTTGTAAATACCAGGAT TTTTCGTTTGTTTGTATGTTTCAGAAGGGAGGACAGACGCTGGAGATAGGAAAGTCTTCAGCATAACCCA TTTGTACCTGACACAAAGGAAGTGTCCCCTCCCAGGCGCCCTCTGGCCCTACAGGTTCAGTCCAGGCTGG CCTTTCAGAAAATTGTTTTAGGTTTGATGTGAACTTGTAGCTGTAAAATGCTGTTAAAAGTTGGACTAAA TGCCTAGTTTTTAGTAACCTGTACATTATGTTGTAAAAAGAACCCCAGTCCCAGTCCCTAGTCCCTCACT TTTTCAAGGGGCATTGACAAACCTGTGTATATTATTTGGCAGTTTGGTATTTGCAGCACCAATCCTTTTT TTTTTTTTCTGTTGTAACTTATGTAGATATTTGGCTTAAATATAGTTCCTAAGAAGCTTCTAATAAATTA TACGAATTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6416507 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6416507 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7640 same embryo
	EMAGE:7642 same embryo
	EMAGE:7638 same embryo
	EMAGE:7639 same embryo
	EMAGE:7641 same embryo
	EurExpress:euxassay_004735 same experiment
	MGI:4826721 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source