EMAGE mouse gene expression spatial database

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EMAGE:7639

Otx1 orthodenticle homolog 1 (Drosophila) ( MGI:97450)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004727. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004727_01	euxassay_004727_02	euxassay_004727_03	euxassay_004727_04

euxassay_004727_05	euxassay_004727_06	euxassay_004727_07	euxassay_004727_08	euxassay_004727_09

euxassay_004727_10	euxassay_004727_11	euxassay_004727_12	euxassay_004727_13	euxassay_004727_14

euxassay_004727_15	euxassay_004727_16	euxassay_004727_17	euxassay_004727_18	euxassay_004727_19

euxassay_004727_20	euxassay_004727_21	euxassay_004727_22	euxassay_004727_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7639_wholemount_strong.wlz

7639_wholemount_moderate.wlz

7639_wholemount_weak.wlz

7639_wholemount_possible.wlz

7639_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7639_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 18 19

spinal cord

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 15 16 17

conjunctival sac

moderate

regional

moderate expression: see section 01 02 03 04 21 22 23

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 22 23

naris

moderate

regional

moderate expression: see section 09 10 11 14 15 16

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 14 15 17 18

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

naso-lacrimal duct

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 16 17 18 19 20 21

pharynx epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13

lower jaw incisor

moderate

regional

moderate expression: see section 15

oral epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8907
Entity Detected:	Otx1, orthodenticle homolog 1 (Drosophila) ( MGI:97450)
Sequence:	sense strand is shown >T8907 GCCACTCCGACAAGGTTGGCTGCCGGCGAGTCTCGGTGAAGGCGAGAGGTAGATGGTGAAAAGCCGCGAC TGCGGAGGGCAGGCCAGGCCCCCAGAAGCAACAGCTTGAAGGACTTCGCAGTGGGAGCCCTGAACTGCTC CTGGCCTCAGGCCCCCTGGATCCGTCGGGGCACCTCCACCCGGCTGTTAGCATGATGTCTTACCTCAAAC AACCCCCATACGGCATGAACGGGCTGGGCCTAGCTGGCCCTGCCATGGACCTCCTGCACCCCTCCGTGGG CTACCCTGCCACCCCGCGGAAGCAGCGACGGGAGCGCACCACCTTCACGCGCTCACAGCTGGACGTGCTC GAGGCGCTGTTCGCAAAGACTCGCTACCCAGACATCTTCATGCGCGAGGAGGTGGCACTCAAGATCAACC TGCCAGAGTCCAGAGTCCAGGTTTGGTTCAAGAACCGCCGCGCCAAGTGCCGCCAGCAGCAGCAGAGCGG GAATGGAACGAAAACCCGGCCGGTCAAGAAGAAGTCGTCTCCAGTACGCGAGAGCTCGGGTTCAGAGAGC AGCGGCCAGTTCACGCCGCCCGCCGTATCTAGCTCTGCTTCTTCGTCTAGCTCAGCGTCCAGTGCCTCCG CCAACCCCGCGGCTGCTGCGGCCGCGGGCCTGGGTGGGAACCCAGTGGCAGCAGCGTCCTCTCTGAGCAC GCCTACTGCTTCGTCCATCTGGAGCCCGGCCTCCATCTCGCCGGGCTCAGCGCCGACATCCGTATCGGTG CCAGAGCCACTGGCCGCTCCGAGCAACGCCTCGTGCATGCAGCGCTCGGTAGCCGCAGGTGCGGCCACTG CCGCAGCCTCCTACCCTATGTCCTATGGCCAGGGCGGAAGCTATGGTCAGGGATACCCCGCGCCCTCCTC TTCTTACTTTGGCGGTGTAGACTGCAGCTCCTACCTAGCGCCCATGCACTCTCATCACCACCCGCACCAG CTTAGCCCCATGGCACCCTCCTCCATGGCTGGCCATCATCACCATCACCCTCATGCGCACCACCCACTGA GCCAATCTTCAGGCCACCACCACCACCATCACCACCACCACCACCACCAAGGTTATGGAGGCTCTGGGCT CGCCTTCAATTCTGCCGATTGCTTGGATTACAAGGAGCCCGCCGCCGCCGCTGCTTCCTCTGCCTGGAAA CTCAATTTCAACTCTCCCGACTGTCTGGACTATAAGGACCAAGCCTCGTGGCGGTTCCAGGTCTTGTGAG CCCAGGAGGGAAAGGAAGTGGAGAAGAAACGCAACTACCTATACCCTCCATGGTCCCCATCCTGTTGCTG CTGCTGCACCACCCGCCTTTGCCTTGGCTTTTCCAAAACTTAATTTTCAGTCCCCCAAAAGATATTCATT GCCTGCACTGCCGCTCCCATCTGTGTGCCACTTGGTTGGGGGTGGAGGATGCAGCCCTTCTCCCCCTTGG AGGTGGGGCCCGGTGCTCCCACTTGGCCCTCCGTTTCTTCCCGGAATAGCAGTACGCTCTCGCTCTCAGC TCGTGCGTCACCCCTTCAAGTCTTTCTGGACAGCTTTTATGCACCTGGAGCCTTCTGGGGCTCTTCGCTC TGACCCTCTGTTTTGAGCTCAACCGTTTTATTTATTCTTTCTGGACACTGAAGTCACTAACTGGCGTATA TCAACCGTTTGGAGTGTGTCCATACATACACACTCCTAATCAAACCCTCCACTGTTTCTCCTAGCAGACT GTCGCCCCTTCAGCTGCCCTTGATCTAGCTCCCCACCCGCAGGCCCGAGCTTCCAGCATTTCTGTCTCAC CAGTGGTGAGCGTTGGGCCAGGCTCGCGGCAGGAGTGTGCCCCAGCCTCCTTTCCTACCCTGCGCTGGAC TGATTCAAGCTTCCGCCTCAGCGGGAATGCTGTACATAGTCAGGTCCGTTCCAGGGACCACTTAAAGTTT TTAGTTGCTGTTGGTTGAACTGAGCATCTCTCTTGTTTTAGCGTCCAAGAAACAGAACTTTAAAATATAT ACATCACATATATATATATATATATACACACGCACACACACACATATATATATAACAGAAGCAAAAAAAT TATTTCCCCTTTGTTCCCGTTCCTCTCTTCCTCAAACGATGGTGGTTCTTATTTTTCAGTTATTACAAAG CTGTCCTGCCCTCTTCACACCTCCCTTTGTGTATTTATTAAAGAAACCCGCTTGGTTCCAGGAAGCTGGG CGCTAGAGGATCGGAAGTGAAGGAAACAGCTAAGTCAGGATACAGGGGTCGGGGCTAGGCTGGTCAGGGC GCACGACTGACCCTTCGCCAGTCTAAGCACAGTATCAAAGCTTCCGCTTAGAAACAAACAGACAAACAAA CAAGGTGAAATTACAGCATTGTGGAAAGAAAACCAAGCCCGAGTTCCAGGTCCAATGTTTCTCTCTCTCT CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAAACACCCTGTCTGTTCTGTGAGTTTTTAGTGA ACCTTCCTTCTCCGAAATCTGCGGAGAGTACCTGTGTACCTATCGGTTTTGGTTTTGGCCGCTTGGTTCA GTAGGTGGGAAAGTAATTTGTAAATTTGATTTGTCCGATGTGAAGATCACAAATTACTTGTTGAGATGTA AGGCAGTCCGTCTCCTCTTCCTCTTTATCTACATTATTTCCCGAAAATAAAAGCAAATTAATGAAAAAAA AAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6416436 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6416436 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7640 same embryo
	EMAGE:7642 same embryo
	EMAGE:7638 same embryo
	EMAGE:7637 same embryo
	EMAGE:7641 same embryo
	EurExpress:euxassay_004727 same experiment
	MGI:4827006 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source