EMAGE mouse gene expression spatial database

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EMAGE:7640

D10Wsu102e DNA segment, Chr 10, Wayne State University 102, expressed ( MGI:106381)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004714. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004714_01	euxassay_004714_02	euxassay_004714_03	euxassay_004714_04

euxassay_004714_05	euxassay_004714_06	euxassay_004714_07	euxassay_004714_08	euxassay_004714_09

euxassay_004714_10	euxassay_004714_11	euxassay_004714_12	euxassay_004714_13	euxassay_004714_14

euxassay_004714_15	euxassay_004714_16	euxassay_004714_17	euxassay_004714_18	euxassay_004714_19

euxassay_004714_20	euxassay_004714_21	euxassay_004714_22	euxassay_004714_23	euxassay_004714_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7640_wholemount_strong.wlz

7640_wholemount_moderate.wlz

7640_wholemount_weak.wlz

7640_wholemount_possible.wlz

7640_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7640_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8908
Entity Detected:	D10Wsu102e, DNA segment, Chr 10, Wayne State University 102, expressed ( MGI:106381)
Sequence:	sense strand is shown >T8908 GGAAGCATGGAGTTCCAGGGCGAGCGCGGGACCGGTCCTGGTGTTTCTTCGTCTTCTGTAGCCTGCTCGC AGGTCACGGTGTCCAGGGAGCTGCTGACGGCGGGGAGCGAGGGTTCGGGAGGTATCTGGGACCAGTTGCT CATCAGCTCTAAGCCTCATCCCAGAAAGACCTCTACACTTCAAACAGTTCGGATGCAGAGGAGCCCTTTG CTAGACCAGGTTCAGGCCTTCCTCCCACAAATGGCCCAGGCAAATGAGAAGCTGAGGAGGGAAATGGCAG CTGCTCCAGCTGGTCACTTCAATATTGAAAACATCGACGAGACGTCTGGAAACATCATACAGATGGATGT GGCCTTATTTGAGATGAGTCGGTCAGATTCAAAAGAGGAGGATAGCCCAGAAGAGAGCTCTCGGGACAGT TCTGGGGACAGCTCGGAGTCCGAAGAAGATGTCTGCGTTCCCTCTGAAGTCACCATCGAGAACATTAAGC TTCCGAATGCAGAAGGTGGCAAAGGCAAGATTGAAATTTTGGACAGTCCGGCAAGTAAAAAAAAGAAGCA GTGAAATGACCCACATGGAAGGAGCAGGCACTTCCCCGCCTGGGGGCCAGGGGCAGGGCGGCCTGTTCCG ATCAGCTGTTGTCCTTACGTGTCACAAAGAATCATGAACCCAGGTGTATTTTACATAGAAAGTGCTCAGA GCTTGGACGCAGAGAGCGTAGTGCTCTTGGACACTGAGAAAGGAAATAAGAAATGACAATATTGGTTGAT GCAGATGTTTGCTCTAGAGTGTGGGCTGTAACTCTTGACTCATCTACACTGAAATGTAGCTATTGGTTAC AAGATTCCCACTGCCAGTTTGGGGTTTTGGTATGTTTTATATCAAGGCAGGTTGCAGTTTGTTATGTATG AACTGGGTACTAGAGACAAGCCCAGGATGACAGCCCCCTGCCATTTAAGGTGTGACGGTGCCTATATTCA GTTCAGGAAAACCCAAGCTGCCTTTTAAAGATTTGTAAGGGATAGCAGTTGACATCCTTTTAGAGCAGAT TGATCTTTGCTCCTTCTGTGAAGAGAGTTTAGAAACAACTATGTGCTATGTCACTGTCTTGTGTGTGAGT GTGAAGTCATGTAAAATTGTCATTTCAAATTATAATGCTACTGACAGCCCGAGGAATGCCTCCAGTTCTA GTAAGCTTACAGTGTGGATGACCTGTAGCTGTTTCACTGACTAACCAGTCTTAGATGATGTTATCAGGGT TAGCAAGTGCCAGCAGCGGGAAGACGAGCGGTCTGTTCAGGAGGCTACTTGAGTAGAGGAACCATGTCCC TGTGACTTGGAGGAGATGGTAACACCCCTGTCGTTTATCCTGGTGGTTTGCCGTACCATGTGATACTTCA TCTTTGCCACCTCTGAGCCAGGGTCTCTGATTGGTCCTGGAAGCTACTGTTCCACAGTCATGCTGGGTTA GGGCATCAAGCCTGGACTACAGTTTTCCCACCCTCTAGCACTTCTCACCCTCTGTACCTTCCTCCATAGT CCCTGAGCCCCTAGAAAGAAAAAGGTATCCTGGTCATTTTCCTCCTGAGAACAGAGCAGAGTAAAATGTT CAAGGCACGCATAACTACCTGACTGAAGAGAGTCTCCGTGTATAGGTCAGATCATCATGGGTTACAATCC TTGCTGCCCAGTGGAAGAGGTTCCCCTCGGTGACTAGTTGATAGCCTCAGTATGACTGATCCCCATATTC AGAGGATAATTGTCTTTTTTATTTCTGTGAAGAGACGCTTCATGACCAAGGCAAATAGTAAGTGTTTAAC TGCGGGCTTCCTTTCAGTTTGAGAGTCTATGACATTCTGGGGGAAATGTGGGGATGGGCAGGGCACCAGG CAGAGCAGAGCAGTGTATAAGACTGGGCCTAGCTGGGCTTTTGAAACCTCAGAAGTCTGTCCATCTCTCC TCCCTCCCCCGTCCAGTGACATACCTTTTCCAACAAGGCTCCTAATTCTTCCTAAACTGTCCGCCAACTA GGAACCAAACATTAAAATCTGTAAGGACCATTCTCATTTAAACAATCACAGTGATTGATTGGGTACGTAC GTGTTTGGACATAGGATTGTCTTTAGCTCGAGGTTGGAGGGGGCTAGACAGACAGTTGGGAGATGGTTAA CTGTGGGCAGATTGAAGCACCACAGAACCTGCTGTTCCTGTGGGACACACAGGTAGTGGAGGACTTGGGA GCCTCAGCCAAGTGAGGCTGTAAGAGTTGGCTTCCCCTCGGGGCCTGAACAACAGCCTCTGACACTGAAT GCTAACTGCTCGGGATTGGGGTAGTGCTTTCTTGTGTAAGCCCAGGTTGGTATTCTGGTCTCCTGAAGTC AGCATTTGGCGTCCTTAGCAGTAGGGGTGACCATTTAGCTCTAGTACACACACAAGTATAGTGACAGTAG TCACTTCTGGGATCTATCTCCCTGGCCAACAACTCACAGGAAACTAGACCACCACCCTGGGATTGGAGTT TTTACTTAATTAACAACCCTGCAGTTGCTGGAAGCACTCTATGCACGTGTAATGAACAGCTGCCGTTTAA ATATATGTGCCATTTCTGTAGTCTGGATTTTTTTTTCCTTTTTTTGGTGGCATGTTACAGTCTTGGCAAA AAGTTCTCTTCACACATTCCTTTTTGTATATACTGATTTTACTGTTGGTGAAGCACATCTGACCAAGCCA GTTCAGTCAGTCAGCAGGGTCTCCAGGGAGTGAGGATTGAGGAGAAAACGTTAGACAGCATGATCACCTG ACTCCACCCTGTCCTGAGACTGAAGCAGCTTCATTTCTCTCCAGCCTGCTTTATATCCTCCTAGGTGCAT CCAAAAAACGTGGTCAGCTCTTAGAGCAAAGACAAAGTAAGCAAGCCAAGTAGTGAACAAGAAGATCACT TAGGGTAGTAAGTAACGTAGTAGGCAAGGAGATGGCCGAAGGTGGTGGTAAAGTCCCATGGTCACTGTTT CTAATATTCTTATCTGAGCATACTTTCTTGTTGGAGCCCAGGGAGAAATGCCAAATTCTAGAAGCAGCAC CAAAAGCCCTCAATACACATCCTGCTAGATTCCACCTGCTTTGGGGTTTCTCTCTGTGGCTAACAATTGC TCCAGTATTATTTTGTACATTGGGCTTACCTTTCTTCATGACAGGGATATGAAACCCATTGGAACTGTCT GTTAAGTGGTTTTATGTCTTGTCCCCCAGCCCTAGGACCCGTTACTTCTAAGGTAAACATTGGGACACTA AGCACACAGTTGCTTAAGCTGTCCCACTCCCTAACCCACCCCCTCCCCAAAATCTACTTTTTGAGGGAGA GCGTCATATTGAAGGAAGTTGTAGGAGTTGAAGGGGGTGTCGGGGTGCCAATGATCAAGATGGATTGTTT ACATGCATGAAATTGTCAAAAATGAATAAATTCTATTTAAACCGTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6416625 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6416625 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7642 same embryo
	EMAGE:7638 same embryo
	EMAGE:7639 same embryo
	EMAGE:7637 same embryo
	EMAGE:7641 same embryo
	EurExpress:euxassay_004714 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source