EMAGE mouse gene expression spatial database

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EMAGE:7720

Zfp27 zinc finger protein 27 ( MGI:99174)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007483. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007483_01	euxassay_007483_02	euxassay_007483_03	euxassay_007483_04

euxassay_007483_05	euxassay_007483_06	euxassay_007483_07	euxassay_007483_08	euxassay_007483_09

euxassay_007483_10	euxassay_007483_11	euxassay_007483_12	euxassay_007483_13	euxassay_007483_14

euxassay_007483_15	euxassay_007483_16	euxassay_007483_17	euxassay_007483_18	euxassay_007483_19

euxassay_007483_20	euxassay_007483_21	euxassay_007483_22	euxassay_007483_23	euxassay_007483_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7720_wholemount_strong.wlz

7720_wholemount_moderate.wlz

7720_wholemount_weak.wlz

7720_wholemount_possible.wlz

7720_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7720_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9022
Entity Detected:	Zfp27, zinc finger protein 27 ( MGI:99174)
Sequence:	sense strand is shown >T9022 TGGGATTTCAATTTCCCGGGTTTCAATCAGCCACACTGAAGGATGATTTTTACTTATTGTAATAATGTAC AACAGTGACAATTTTTGGAATTTTCGTTTCTTTAAAGAACACAGAAATGTTGTAAGAATGTGTTTTTGTT TTAATCCCAGGTGTGAGATACGGAGCTGCTTCAGACTGTCCGCAGCAGCTGATCATGATTTGCCTCGTGC TCTAGCAGGGGTGTGATTTTTGCCAGCAGCAGATAGTTTCTGAATTAGTGTCCTTCATGGATGTGACTAT AGATTTCAGCAGAGAGGAGTGGCAGCATCTAGATCCTGCTCAAAGGAGTCTCTATCGGGATGTGGTGCAG GAGACCTACAGTCACCTGCGCTCAGTGGAAGAACTACAGCAGATTGGTGACCAGAAAAAGACCTACCAGC AAATAGGACATAAATCAGCGAGTGATATGGTTTTCATCACGAAGACGCTGGGTGCAGAGAGTTGCCATGA CTACTCAGGCGTTAGGAAGGTGATTCATGTGAACTCATACATTGTTCTCCCTCCCAAGAGGCCACGTCAC TGGGACCCACCTGAGGATGAGCCGAAGCATAGTTCAGATTTACAGACTCATGATGAAAGTAATGGGCTGA AGAGAACTAAAAGGATTACTGAATATGGTAAAATTTCATCCTGTATCAACACTGAACATATTCTAACAGG AGAGAAATTACCGGACCATAATCAATGTGGAAAAGTCCTCGGTTATAAACAAATACCTTGTCAATATCAG AAAATTCATACTGGGGAGAAATCATATGAATGTGCTGAATTTGGAAAGATTTTTACCCAGAAGTCACAGC TCAGAGTACACGTGACGTCTCCTACAGGAGAAAAACTCTACGTGTGCGTCGAATGTGGGAAGGCATGTTC TCAGACGTCAGAGTTCCTCACACATCAAAAAACCCACACCAGGGAGAAGCCCTATAAATGCGGTGACTGT GGAAAGTCCTTTTTCCAAGTATCTTCTCTGTTCAGGCACCGGAGGATCCACACTGGAGAAAAGCTCTACG ACTGTAGCCATTGTGGGAAAGGCTTCTCTTACAACTCAGACCTCAGGATACATCAGAAGATCCATACAGG AGAGAAACGCCATGGCTGCGTCGACTGTGGCAAAGCCTTCACACAAAAGTCCACACTGAGGATGCACCAG AAGATCCACACGGGCGAGAGGGCCTATGTCTGTATTGAGTGTGGACAGGCTTTTATCCAGAAGACACACC TGGTCGCACACCGAAGAATTCACACTGGAGAGAAGCCATACGCGTGTGACGGCTGTGGCAAAGCCTTCCT TTCCAAGTCCCAACTCCTGGTCCACCAGCGAATTCACTCAAGAGTGAGGCCCTGTGTATCTCTCGACCGT GCGAAACCCTTCAGCAGCGCTCCCAACCTCCTTCCGCGTAAGAAAGTTCAAATGAGAGAGAAATCGTCCA TCTGCGCGGAGTGTGGGAAGGCCTTTACCTACAGGTCGGAGCTAATCATTCATCAGAGAACTCACACGGG AGAGAAGCCTTATCAGTGCGGGGACTGTGGCAAAGCCTTCACCCAGAAGTCAGCCCTCACCGTGCACCGA AGAATCCACACGGGGGAGAAGTCGTATGTGTGTGTGAAGTGCGGGCTAGCCTTCGTGCAAAGGGCACACT TGGATGCACACCAGGTGATTCACACTGGAGAAAAACCTTACCAGTGTGGTCATTGTGGGAAATTCTTCAC TTCCAAGTCGCAACTCCACGTGCACAAGCGGATTCACACGGGGGAAAAACCCTATGTGTGCAGTAACTGT GGGAAGGCCTTTGCCAACAGGTCAAATCTCATCACACATCAGAAAACTCATACAGGGGAGAAAGCCTATG TCTGTGCAAGGTGTGGGAAAGCATTCACTCAGAGGTCAGACCTGGTTACACACCAGAGGATACATACCGG AGAGAAGCCATATGGGTGTAGCACCTGCGGGAAAGCCTTTACCCAGAAATCACACCTCAGTATACACGAG AAGATTCACACCGGAGAGAGACAGTACGGATGCCGAGACTGTGGGAAAGCCTTCAACCAGAAATCCATAC TCATCGTGCACCAGAAAATCCACACGGGGGAGAAACCGCACGTGTGTGCCGAGTGCGGGCGAGCTTTCAT CCGCAAGTCCAACTTCATCACCCATCAGCGCATCCATACTGGCGAGAAACCTTATGGATGCACCGACTGC GGCAAGTCCTTTACCTCCAAGTCGCAGCTCCTGGTGCACCGGCCAATCCACACCGGCGAGAAACCTTACG TGTGTGCCGAGTGTGGGAAAGCCTTTAGCGGCAGGTCAAATCTCAGCAAGCACCAGAAGACTCACACTGG GGAGAAGCCGTACGCGTGTTCAGAGTGTGGGAAGTCCTTCAGGCAGAAGTCAGAGCTGATTACACACCAC AGAATCCACACTGGGGAGAAGCCGTACGATTGCGGCGACTGCGGGAAATCCTTCACTAAGAAATCCCAGC TCCAAGTGCATCAGCGAATTCACACAGGAGAGAAGCCGTACAGATGTGCCGAGTGCGGGAAGGCCTTCAC CGATAGGTCCAATTTGAATAAACATCAAACAACACACACCGGAGAGAAACCTTATAAGTGTGTAGTCTGT GGGAAAGGCTTTGTGCAGAAGTCAGTGCTCAGCATTCATGAGAATGTTCACACCTCGGCAGTATGAGAAA GGACCCATTGAGGACACGTCTTATGTAATTACTGTCTCTATAGGACAGAATAATATGTATATTATTGTAA GTAAAAATGCCACATGACAGTGTCACACATTACTGCTCAGAAAAAGATCTCAGAGAAGGCACTGAATGGG ATTATGGGGCAGGACATTTCTACTTGCTGAGAAGAAATTGCATCAATTTGACACAATAGAAGCGTTCTCA TGGTAAAAAACACAGTGGAACATTGTCATCAAGTTCTTCACAAGAAAGATTGTGTCAACTCATGTTGATG CAAAAATCCTGTTGGTGGAGTATGTCTACTGCCAACTAATTACATGGTAATCTAATCTAATATAATGTAC ATGACAGGGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6812360 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6812360 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7723 same embryo
	EMAGE:7722 same embryo
	EMAGE:7725 same embryo
	EMAGE:7724 same embryo
	EMAGE:7721 same embryo
	EurExpress:euxassay_007483 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source