EMAGE mouse gene expression spatial database

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EMAGE:7723

Ubr7 ubiquitin protein ligase E3 component n-recognin 7 (putative) ( MGI:1913872)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007452. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007452_01	euxassay_007452_02	euxassay_007452_03	euxassay_007452_04

euxassay_007452_05	euxassay_007452_06	euxassay_007452_07	euxassay_007452_08	euxassay_007452_09

euxassay_007452_10	euxassay_007452_11	euxassay_007452_12	euxassay_007452_13	euxassay_007452_14

euxassay_007452_15	euxassay_007452_16	euxassay_007452_17	euxassay_007452_18	euxassay_007452_19

euxassay_007452_20	euxassay_007452_21	euxassay_007452_22	euxassay_007452_23	euxassay_007452_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7723_wholemount_strong.wlz

7723_wholemount_moderate.wlz

7723_wholemount_weak.wlz

7723_wholemount_possible.wlz

7723_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7723_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9021
Entity Detected:	Ubr7, ubiquitin protein ligase E3 component n-recognin 7 (putative) ( MGI:1913872)
Sequence:	sense strand is shown >T9021 TGACAGTTGAGGATGGCCGGAGCCGAGAGCCCCACCGAGTGCCAGGCGGAGCTGGAGCCCGTGGTGTCGC TGGTGGACGTGCTCGAGGAAGACGAGGAGTTGGAGAATGAGGCGTGCGCCGTCCTGGGCGGCAGCGACTC GGAGAAGTGCTCCTACTCGCAGGGCTCAGTGGGGAGACAAGCACTGTACGCCTGCAGCACCTGCACGCCA GAGGGAGAAGAGCCCGCAGGGATTTGTCTAGCTTGCAGTTACGAATGTCATGGAAGCCATAAACTGTTTG AACTATACACAAAAAGAAATTTTCGTTGTGATTGTGGAAACAGCAAGTTTAAAAACTTGGAATGCAAATT ATTTCCTGACAAATCAAAGGTAAATTCTTGCAATAAGTACAATGACAACTTTTTTGGATTATACTGCGTT TGTAAGAGACCTTATCCTGATCCTGAAGATGAGGTTCCAGATGAGATGATACAGTGTGTGGTCTGTGAAG ACTGGTTCCATGGCAGGCATCTTGGTGCCATTCCCCCTGAGAGTGGGGATTTCCAGGAGATGGTGTGCCA GGCTTGTATGAGGCGCTGTTCTTTCTTGTGGGCTTATGCTGCCCAGTTGGCAGTCACCAGGATATCTGCT GAAGATGATGGGCTGCTGCCAAATGCTACTGGCATGGGAGATGAAGATGTTAGCAAGCCTGAAAATGGAG CTCCCCAAGATAATGGCCTGAAAGAGGATGCTCCCGAGCATGGCAGGGATTCTGTCAACGAGGTGAAAGC TGAGCAGAAGAACGAGCCCTGCAGCAGCTCTAGTTCTGAGTCTGACCTCCAGACAGTGTTTAAGAAAGAA AATATCAAAACTGAGCCACAGTCCAGCTGCAGACTTCAGGAACTCCAAGCCAAGCAGTTTGTAAAGAAAG ATGCTGCCACCTATTGGCCCTTGAACTGGCGCAGCAAACTATGCACCTGTCAAGACTGTATGAAAATGTA TGGAGAACTAGACGTCCTGTTCCTGACAGATGAATGTGATACAGTTCTGGCTTATGAGAACAAAGGCAAG AACGACCAGGCAACTGATAGAAGGGACCCTTTAATGGACACCCTTAGCAGCATGAATAGAGTCCAGCAAG TGGAGCTCATCTGTGAATACAATGACTTGAAGACTGAACTTAAGGACTACCTGAAGAGATTTGCTGATGA AGGCACGGTTGTTAAGAGAGAAGACATTCAGCAGTTCTTTGAAGAGTTTCAATCAAAAAAGAGAAGGCGA GTGGACGGGTTACAGTATTACTGCAGCTAAAGTGGAATGCCACTTCCTCGGTCAGGTTCACGAAAATGCC ACTGTTCCGGAATAAAGAGACCTGTGTCACACTTGGTCCCCTTTCCATCCTCCCGTGGCCTCAGGCCTTC TCCTTAAGTAGCTGCAGGGTTTCTACTGGTCTCTCAGCAAGCGGCCTCTGTGACAGCTCATTCCACAGAC GTCAGCTCTCCTGCCCAGGATGCCCAAGACTTCTTTCTGTTCATGGTTTGAGAAGTTTGATTTGATCTTG TGAAAGCAAATTAGCTTCTTTTCTAGCAGTTTGTTCCATGACTTCCATATGTACGCTCACAGAGCTATTG CCCTCTGGGTCTTCTGGGTCTTGCAGGGTCTCTTCCTGTTAGCCCGAACCTCTGGGGAGCAGTCCCCTCT GCCCCATGATCACGGTGCCTTGGGTCAAAGCTTCCTCAAGCCCGCTCTGCCAGTCTGCTCAGCGCTTTCT GAGATTCGATCGTGGCTTGAAGAGATGGGACTTTTCTCTGTACCGACCTTCACGGTAGCCGCGACTGATG TCGTCTGTGGGAGGTTGGCTTAAGGCAGGATTGAGGAAGCAAGCCTTTGTTCTGAATTTCGCTGATTAGT TTGAAGTGAAATTTCATTACTATTCTAATGGCCCTCCAACATCAGCGTTAGTACTGTACTGCTCCCAGGG CTTCCAGGGAATCTACTCATGTGAGCCCCTTTGCTGCTGCCTAAAAATGACCAGATCCTACCCCCTAACG TGGGTGCTTTACAGAGTCCTCTGGTTCCTTACATTATCAGGGATATGCAGAAACATATATTAAAAATGGA CCTATTTTAATACTGTTCTGTCAGGAAAATGTTATTGGAACCTGAATATTTTTCCCCTTGAATCTGCTGA AGGCAAGGATACTGACTGACCATAGTGGTAGTAAGTGGGGCAGTGACAGCCCTAGAAGATGGAGGATTCC AGCCAAAGCCCTCAGCTATATACATATTGAGTCTTTGTAAAAGTGCGCACAGCCACTCCCTCCTGACGGC TCGGAGGGTCTTCATGGATTGTTGTATAAAGTCCTGTGTTTGAGAGAAGTCTTTCAGATGGTGTGAAGTC TTTTTAAGCTCCCTCAGTCTATGTGGAAAACATCTTTTGATCCACAAAGACCTAAGTGGCACCCTCCAGA GGAACAAGGTCTTCCCTAGTACACGGTGAGGTACATTAAAGACATTTTAGGCCACATGTTGGTGGGGATT TTTGTGTGTGGGCAGCAGTTGTATGCATGAATACTCGAGTTCCTTGTCACGATGAAGTAGCCTACAAATA AGGCCTCAGGAAATGGCATGGCTTCGGTACTGTATACAGCTTGCAGCCTTCTAGTGACAGATCCTGGGAA GACTGCTATGTGGAGCTGCAAGGTTCTTCCCGCTTCAGGCGAAGCCTGTGTGCACTGAGCTGGCCTGGAT AGGGCTCAGGCAGACCCCCGGTGCCCACAGCCTCGGGGTCTCCTGAGCTGAGGATTTGAATTTGAGAGTT GCACTTTCTATGTACCAGCACATCCTCAAAACTTCTCTCTTCCCAGATGTCAGATTTAACCACCAAGTTC CTGTCTGCCCCTGATAGGTCAGAACCAAGGCTTAGCAGCAGTGTCAGTGAGCAGCAAAAATACAGCCAGC CTATGTTGAAGGGCTTGCATAAGAAAGGTTCCCTACTTAACCAGAAAACCTCTTTTTGTACCTATTCACA ACTAGTTTTCTTACTATTCTCGTAGAGATGGTTTGGGCCAACATGTGGCAGTTGATATTAATGCATGTTT TATGCAGAAGAAAATGTGATACTAGTTGCTTTTAAGGAATTCTGGCATTGTATGTACATTTTTGTAGAAA TTATTACTCAATATACTTCAATCAGGTTTCTCTAAAATCCAAATTAAACCTATTCAAAATAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6812389 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6812389 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7722 same embryo
	EMAGE:7725 same embryo
	EMAGE:7724 same embryo
	EMAGE:7721 same embryo
	EMAGE:7720 same embryo
	EurExpress:euxassay_007452 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source