EMAGE mouse gene expression spatial database

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EMAGE:7725

Ptp4a2 protein tyrosine phosphatase 4a2 ( MGI:1277117)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007473. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007473_01	euxassay_007473_02	euxassay_007473_03	euxassay_007473_04

euxassay_007473_05	euxassay_007473_06	euxassay_007473_07	euxassay_007473_08	euxassay_007473_09

euxassay_007473_10	euxassay_007473_11	euxassay_007473_12	euxassay_007473_13	euxassay_007473_14

euxassay_007473_15	euxassay_007473_16	euxassay_007473_17	euxassay_007473_18	euxassay_007473_19

euxassay_007473_20	euxassay_007473_21	euxassay_007473_22	euxassay_007473_23	euxassay_007473_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7725_wholemount_strong.wlz

7725_wholemount_moderate.wlz

7725_wholemount_weak.wlz

7725_wholemount_possible.wlz

7725_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7725_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8997
Entity Detected:	Ptp4a2, protein tyrosine phosphatase 4a2 ( MGI:1277117)
Sequence:	sense strand is shown >T8997 TTTTTAATGTTTTCTGCTGCGGAACATTTCAAGATTTATTGATTTCCTCCTCCCCCCATTTTTTTCCCAC CACACTCACACACGCACGCTCACACTTTTTATTTGCCATAATGAACCGTCCAGCCCCTGTGGAGATCTCT TATGAGAACATGCGTTTTCTGATAACTCACAACCCCACCAATGCGACTCTCAACAAGTTCACAGAGGAAC TTAAGAAGTACGGAGTGACAACTTTGGTTCGAGTTTGTGATGCTACATATGATAAAGCTCCAGTTGAAAA AGAAGGAATCCACGTTCTAGATTGGCCGTTTGATGATGGAGCTCCACCCCCTAATCAGATAGTAGATGAT TGGCTAAACCTGTTAAAAACCAAATTTCGTGAAGAGCCAGGCTGTTGTGTTGCAGTGCATTGTGTTGCAG GATTGGGAAGGGCTCCTGTGCTAGTTGCGCTTGCATTGATTGAATGCGGAATGAAGTATGAAGATGCTGT TCAATTTATAAGACAAAAAAGAAGAGGAGCATTCAATTCCAAACAGCTGCTTTACTTGGAGAAGTACCGA CCTAAGATGCGGTTACGCTTCAGAGATACCAATGGGCACTGCTGTGTTCAGTAGAAGTAGAAGCAGGCTG GCTGGATCGTGGCATTAGAGGGAACTCTTGGTACCTGGAAATGTGAATCTGGATTCTTACCTGTGTCATC AGAGTAGTAATGGATTCAGTACTCCTCAACTCCTAATGATTGAGAAGAAGGCAAACGATAAAGAAATCCC TCTATTACACGAATAAAATGTTTAAGAAAAGAAAAAAGAAAGGAAAGGGGATTAATTTAGTGAAAGATGA TTTTGCTCCTAGTTTTGATTTCTGCCAGGATTGAATTATTTCAAAATCTCCTGTGTTTTTTAAGCTTTTT CAAAATAGATGTCTGAGGAAAACCAGCAGAATATTAACCAGTGTGGAGCCAGTTGTTGGGAGCACACACT TCCATTATGCTTGGCACATAGGTCTCCTTGTGGTGGGTGGGATCTGGGGGAGGTAGATTTTCCTCATCTC TCTTCCTGCCTCCCTCCCATCTCCTGATCCACATAGATGGAATAGAAAGCAGCCTTTGTTGCTGTAGATG TGCGTGTGTCTGGCAGCCTTAAGCCCACCTGGGCACTTTTAGGGAAAAAAAAAAAAAACAAAAAAAACCA ACAAAAAAATAAATAAAAATAAAAGCAAAGAAAAGGCAGTGGCGCATATATTCTGTGGTCCAGGCTGTTT CTAGTCTATTGATGATGGATGCTTATATTAGTTTTTTTTTTTAACCCCCTTGTGCCCCTGATCTACCATT AGGGTGTGAGAGGTAGCCAGGAACCTTTGGTTTTATAAATGGTGACGAAATGACATCCTAAACAGAGTCT TTCTCCTGTAATTTAGCTGAGAGAATTCTTTTTCCCAACAGTGAAACAAGTGGGCCTCCTAGAAACTTTA TTGCAGAGATGACTGGAGGCCATTGGTGTTTCCAGGAGAGCTAGCATGTTTCTACAAGTGTAGGACTGGA TTGTGGATCTGCTATCTAACAATGTCATGGAAAAGTGGTATCTTAAAATTTTAGGACCGATTTCAGAAAT AGTCAAAGGAGCAACAGAACATTCATTGTATTTTGGTCTTGAGAAATGCCTATAGTTTTCCAAATTATTC TTGTACAGTTTTATTTGTACAGTTCAGTGTAACCACCTATCATCTTGTTAACACTAGATTCTTCTGAGAT TCAAAAAGAAAAATCACTGTCAGAGCTAGCCTTGCCTAGTGAGTCTGTGTCCACAGGCTGTAACAGGGTG GAAGAGCAACAGTAGGCAGCCCAGGGAGCAGCATGTGCTCACGGCATCATGTGGCAGTGGTGTGGGGTCT CTGCTCAGTGTGTTGATACAGAAAAGCTGTTTTTCCTTGTTTGCAGAAGTTGACCAGACAAAGCAAATGG AGGGGCACCTCTGTGTACCCTCTTCACCTAAGTGTTTATAACCAGCAGTACCCTGCTACAAGCCTTGGGT GCAGCCATCACTGGATTTTGATCGTCAGGCCAGCAGGATGGATGACATCTCATTGATAGTACCCAGATGT TGGTGGCTTTCTGAGTTGCCTGTTTACTGTTGATGCAATTGTTTTGATTTTTAACTAAATGCAAATACCT TCCTGCCTCATCCCAGAAAATGAAAAATAATGCTTTTGACACTGTTTCTAGAATTTTAAAGTTGAAAAAT GATGATATAATCCAGAAATTGTTACTTCGGAGCATGACAATAGATTTCTTTAACAAAGACTCAAGATCAA ATTTCTTACCTAAAAGTTTAGGTTAACTGTTGACTCCTCAAGGGTTTTGATCAATATCACCCAAACCTTT TCCACTTGACATGCTAGAATTTTGGTGTGTGTGTGTGTGTGTGTGTATTTGTTCACGAGCACAATAGCTC CCATGGGGGTCTGCACATGCAGGTGTCCACCAGCATAGCCTGCCTGAGATAGTGTCATTCCTGGTTCTGG GGAGAAGGGTGTGTGAAGGAGAATAAAGCAGCCCTTTGTCTTAAAGAAGACTAATGTTAATTGTTCTCAG AACTGGACGTTTTTTTTCCCCCCATCAAAATTAAAACTCTTTCTTTTGGATTTAATGAAGTATTGCTAAC TGAAGCCAGTTTGACTTGTGAGAGAGGTTAGACTGACAGAAGGTGTGCAGCCTGATTTACAACCAAACCC TGAACCCTTTTGAAGAACAATAAAACATATTTTACAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6811716 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6811716 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7723 same embryo
	EMAGE:7722 same embryo
	EMAGE:7724 same embryo
	EMAGE:7721 same embryo
	EMAGE:7720 same embryo
	EurExpress:euxassay_007473 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source