EMAGE mouse gene expression spatial database

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EMAGE:7780

Itprip inositol 1,4,5-triphosphate receptor interacting protein ( MGI:3042776)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007490. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007490_01	euxassay_007490_02	euxassay_007490_03	euxassay_007490_04

euxassay_007490_05	euxassay_007490_06	euxassay_007490_07	euxassay_007490_08	euxassay_007490_09

euxassay_007490_10	euxassay_007490_11	euxassay_007490_12	euxassay_007490_13	euxassay_007490_14

euxassay_007490_15	euxassay_007490_16	euxassay_007490_17	euxassay_007490_18	euxassay_007490_19

euxassay_007490_20	euxassay_007490_21	euxassay_007490_22	euxassay_007490_23	euxassay_007490_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7780_wholemount_strong.wlz

7780_wholemount_moderate.wlz

7780_wholemount_weak.wlz

7780_wholemount_possible.wlz

7780_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7780_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9036
Entity Detected:	Itprip, inositol 1,4,5-triphosphate receptor interacting protein ( MGI:3042776)
Sequence:	sense strand is shown >T9036 GTTGCCCAGTAAAGCTGGGCGGGAGGAGTCCAGGAGGCGGGAGCCGCCTTGGTCTAGTCGCCCGCATCCC TGGATCCCCGGCAGCCTGCAGACCCGGGACCACGACCCGCGCTTGGAGCGAGGCCCACTCGGTATCCCTG CGGCGGCCGCGGCCGAACGACAGAGACTGCAGCTATGGCCATGGAACTCTTCCGGGTCTGTCTGGTCGTG GTGACAGCCATCATTAACCACCCTCTGCTGTTCCCACGGGAGAATGCCACGATTCCCGAGAACGAGGAAG AGATCATACGCAAGATGCAGGAGCACCAGGAGAAGCTGCGCCTGGAGCAGCTACGCCTGGAGGAGGAGGT GTCACGTCTGGAGGCCGAGAAGGAGGCTCTGCGGCAGGTGGAGGAGGAGCAGCAGCAGCTAGAGGCCCAC ACCGCCTGGGACCTCTGGACCACACTCTGCATGGTCCTCTTCCTGATCATCGAGGTGTTGCGACAGAACC ATCAGGAGGGCACCTTCCCGGAGTGCCTGGGTGGAGACGAGGACGAGCTGTCGGGACTAGGGGGCACGCT GCTGCAGGGCCTTCCCCTGCCCAATAGGGCCACCCTGGACCACTTCTACGAGCACTGTATCCGGAGCACC ACGGGGGATGCCACCCGCACCCAGGAGTTTGTGGAAGGTTTCGTGGATGATCTGCTGGAAGCCCTGAGGA GCACCTACAACGGGAAAACCGACATGGAATTGGAGGACTTCATCGGCGTGGGCAGCATGTATGAGAACTG GCAAGTGGAGAGGCCACTACGGTGCCACTTGTTTATACCCTTCATACCCCCAGAGTTCTGGTGCTCCAGC CTTTCAACGCCCCTGGAACGTCAGGGCTATGGCCAGATCAAGGTGACGCTGGCTGACGGGAACCCTCTAG GCTGTGTCTGCGGCAAGGCTAAGCTTGAGGAGGATATGTTGTGTCTCCTCTACGGCAAGAATAGAGGGGC GTGGCCCAGCAGTGCCGGGTGCGGTGAGATGGAGGGCTTGCTGTGTTCCAGAGAGTCCTCCTATCTCGAT GTCATGCAGGTCATGAAGTGGTTTCAGATGGCCCTCACCAGAGCGTGGCACCGCATCGCCCACAAGTATG AGTTTGACCTTGCCTTTGGAGAGCTGGACACCCCAGGGTCTCTCAAAATCAAGTTCCGCTCGGGGAAGTC CATGCCTTTCATCTTGACTCCTGTGATCCAGTGTAATGACTCAGACCTGTACTTCATCTTACAACTTCCC AAGGAGCCCTGTGGGGGAGGCCCAGCCTCCAGCGCTCACTGGCTCCTGTCCTTTGCTGTCTATGAGCGAG AGTTCCTCCGGATGACAGGCAAGGCTTTGCCGGAGGGTGCCTGCCACCTGAGCTGTTTGCAGATCGCCTC CTTCTTGCTCTCCAAGCAGACCCGTCTGACGGGTCCCAGTGGGCTGAGTGACTACCACCTAAAGACTGCC CTGCTGCATCTCCTGCTGTCCCGGCAGGCCTCTGACTGGAAGGCCAGCAAGCTGGATGTACGCCTGCAGG ACCTCTTCTGCTTCCTGGAGAGGAGCCTGCTGGAGAAGAAACTCTATCACTTCTTCATGGGCAACCACAA GGTGCCTGAGGCTCTGGGTCTCCCAGAGGTTGTGCGCAGAGCCGAGCCTCTCAATCTCTTCCGGCCCTTC GTCTTGCAGCGGACTCTTTACCGGAACACAGTGGACTCCTTCTATGAAATGCTTAAGAACGCCCCGGCGC TCATTAGCGAGTATTCCCTCCATGTCCCTTCAGTTCGTGCCAGCCCACCACCAAAAGCTGTCGTCTCGTA ATAGTTTAAGATTTGGGGGTTGAGGAGGGAGAGCCATCCCGTGCGTCGACCTTCCTGGGCCCTGTGACCT CACACAGGAAACACAGTAGATCTAGAGGGAGCCGAGGCTCAGTTTCCCAGGAAGCCAGGCCCTACAGAGT GAAGGAAACAAGAGTTACAACTAGACGCTGGTTTGCCTTTCCGCCACGGGGGCTTGTTGGTAAAACCATT GTTTGGGTTTGGGTACCAATCGTTTGTAGCTTAGTTTTAGATCACTATGAACGGCCAAGACAGTGACCAC ACAGTCTCTAGGGTACTGAGTTAGAGAGAGCGCAAGCACTGACCAGAATGCAGTCCTTTCTGTAGCATAG TCCGTTCTGTGTTCCATAGCAAAGAGGAGCTACCAGGATGGTGCTGGGGATCTGAGACCCAGCACTGCAC CCCAGGCGTTCCAGGCCCCAGCCATCTGCAGATGAATCTTGAATGGGGACAGATGTGGGAATTCACAGCC TTGGAACCGCATCGTGCACAGTAATAGAGGTCCCACAGTGGCTGAGTTGGCTAAAAGAACCTGCCAGAAG GCTAGGGGCCTGGTGGCTAATGTCTCCACTACAGAGAAGTGGGGCCTTGGTGGTGGAGTGTCTAAAGCTG GCCAGTTTCTCAGGCACACATCTTACTCAGGTGCATCCCCTGCTGCTCTACCCTTGTATGGGGTCCCAAT CACAGTGGGTGCCATTTACCACACTGGGTGCTGGCCCAGAAGCCCCTCTCCTCTCTCCGTTGCTGGTCTT ACCAGCCAGCAGTATGGCAGAAGGACACAGGGACAAAGTTCGACCCACTTTGAGGAAAGCTAGAGATCCC TGGGACAAGGGTTAGGATATCTCTAACCCATCTTGGGGTCCTGGTCATAGGGAAAGGTGAGCTTATAAGC CACAGGCACTATGAAAACCCAGCTCTGGTCCTCGGACCCCGAGCATCTCCCAGTTCCTAATGCCAAGAGG GCCTCACCCAGTGCCTCCAGTCTTTTCTGCACACGCCATGGTGACCTCGTAGCTGACCCAGACTTAAAGA AGGTGGTAGAGGCTGGGCTCACTGGGGATCTATATGAGTCGTTCAAGCATAGCCTGGTTACCCAAAGGAG CGTTGTGAGCCCCAGACAAGACCAGAGCCTGAGGCTGGACTCCAGGACCCAGCAGCTTCTTTTCCACTTG ACCTTGACTGTCTGTTTGGGATTTCTTCGCCACATCTCCTTAGGAACTTTTACAGTGGGCCAAAGAAGAA ACAGTAAGAAAGAGGTAGCGCCTGCTAGCCTTCCAGAAGTGCAGGCATCAAAATCAGGCTTCCCTTCGCC CCACACGTGCCTCCTGTGGTCTCAAAGCTCTCCAGAAGATAGACGATTTTGCCACCAGGGTTAAGTGGTG GTGGAGAGGATCTGCTGGGTGCACAGAAGCCAGAGGCAATAGGATACTTGAGTACCGGCGGTGGGGGTTA AACTGAGCATGCGCGGTCCTGCCCCTCCGCAGCTCCAATTGTCACCCTAGCACCCAGTCATGGAGCCTAC TCAATGTGCAGCTCTGTCAACAGCCCTAAGCCGCCTCACCCGAGAGGTACCTTCCAGAGGGGAGGCAGAT GTCCTCATTGTCACCTGTGCCTTTCCTGGCTCACTCATATCCGGCTGTGTTTCCAGGAGTGCCCGGCAAC CAAGAACCAGCCTCCAGAAGCCAAGACCCAACCTGAGCCTCCTTCAGCTTCCCCTTCGCTTCCAGGGAAA GATCACGGGAAGGCCATGGGCAGAACATCAGTGGCTCCTTGTCTGCTGAGCACCCGGGCAGGACAGGAGA CTGTCCCACACCAGGGCTGCTTTGGAAGCAGCAGGGCTCTTAGGGGTTCATGCTGCCTTGTTTGTATTAA AAGAAGAATTAAATTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6813779 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6813779 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7778 same embryo
	EMAGE:7776 same embryo
	EMAGE:7775 same embryo
	EMAGE:7777 same embryo
	EMAGE:7779 same embryo
	EurExpress:euxassay_007490 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source