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EMAGE:7904

Ms4a3 membrane-spanning 4-domains, subfamily A, member 3 ( MGI:2158468)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7904 EMAGE:7904 EMAGE:7904 EMAGE:7904 EMAGE:7904
"Pseudo-wholemount" of euxassay_012966. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012966_01 euxassay_012966_02 euxassay_012966_03 euxassay_012966_04
EMAGE:7904 EMAGE:7904 EMAGE:7904 EMAGE:7904 EMAGE:7904
euxassay_012966_05 euxassay_012966_06 euxassay_012966_07 euxassay_012966_08 euxassay_012966_09
EMAGE:7904 EMAGE:7904 EMAGE:7904 EMAGE:7904 EMAGE:7904
euxassay_012966_10 euxassay_012966_11 euxassay_012966_12 euxassay_012966_13 euxassay_012966_14
EMAGE:7904 EMAGE:7904 EMAGE:7904 EMAGE:7904 EMAGE:7904
euxassay_012966_15 euxassay_012966_16 euxassay_012966_17 euxassay_012966_18 euxassay_012966_19
EMAGE:7904 EMAGE:7904 EMAGE:7904 EMAGE:7904
euxassay_012966_20 euxassay_012966_21 euxassay_012966_22 euxassay_012966_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7904Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7904_wholemount_strong.wlz
7904_wholemount_moderate.wlz
7904_wholemount_weak.wlz
7904_wholemount_possible.wlz
7904_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7904_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
liver
strong strong
spottedstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39081
Entity Detected:Ms4a3, membrane-spanning 4-domains, subfamily A, member 3 ( MGI:2158468)
Sequence:sense strand is shown

>T39081
GAGGAAGAGACTGCTGGTTTTGGGGGACAGACTCTGGTGGTCATTACTGTCTCCTCTTCTGTAATGAGTT
GGACTTGCAGGGGAAGGACTTGTCAAATCCTGAATTCTTCAAGGACTGGGGATCTCGGCAGGTGGTACCT
CATCAGGCAGACACATGAAGCCAGAGGAGACTGGTGGTTCTGTTTATCAGCCCTTGGATGAGTCACGCCA
TGTTCAACGAGGTGTACTGCAAGCCCTCGGGGCCATCCAGATCCTGAATGGAATACTGATTCTAGCTCTC
GGAATTTTTCTGGTTTGTTTACAACACGTGTCCCACCACTTCAGGCATTTCTTCTTCTTCACCTTCTACA
CAGGCTACCCACTGTGGGGTGCTGTGTTTTTTATCAGCTCAGGATCCTTGACTGTTGCCGCAGGGAGAAA
CCCCACACGAATGCTGATGCAAAACAGTTTTGGGATAAACATTGCCAGTACTACAATTGCATTTGTTGGG
ACTGTTTTCCTTTCTGTGCATTTGGCATTCAATACCCAGGCTTTCAAGGGTTGCCAATCTTCACCGTCAC
CTGATGTCTGCATTTCCCTGGGTTCCTCATCAGATGGCCTGGTGTCTTTAATGCTGATTCTCACCCTGCT
GGAGCTGTCCGTGACCATTTCTATCTCTGCCATGTGGTGCTTGGGAAATGTTTGTGGTTTAAGAGAGGCA
ATTACTTCACCTCCTAATTCTGTGGAGTCAGGAATACTTCCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95575. Forward Primer - name:095575_F_cDNA_Ms4a3, sequence:GAGGAAGAGACTGCTGGTTTTG; Reverse Primer - name:095575_N_SP6_cDNA_Ms4a3, sequence:AGGAAGTATTCCTGACTCCACA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7905 same embryo
 EMAGE:7903 same embryo
 EMAGE:7906 same embryo
 EurExpress:euxassay_012966 same experiment
 MGI:4826462 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS