Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7905

Olfr1494 olfactory receptor 1494 ( MGI:3031328)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7905 EMAGE:7905 EMAGE:7905 EMAGE:7905 EMAGE:7905
"Pseudo-wholemount" of euxassay_013010. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013010_01 euxassay_013010_02 euxassay_013010_03 euxassay_013010_04
EMAGE:7905 EMAGE:7905 EMAGE:7905 EMAGE:7905 EMAGE:7905
euxassay_013010_05 euxassay_013010_06 euxassay_013010_07 euxassay_013010_08 euxassay_013010_09
EMAGE:7905 EMAGE:7905 EMAGE:7905 EMAGE:7905 EMAGE:7905
euxassay_013010_10 euxassay_013010_11 euxassay_013010_12 euxassay_013010_13 euxassay_013010_14
EMAGE:7905 EMAGE:7905 EMAGE:7905 EMAGE:7905 EMAGE:7905
euxassay_013010_15 euxassay_013010_16 euxassay_013010_17 euxassay_013010_18 euxassay_013010_19
EMAGE:7905 EMAGE:7905 EMAGE:7905 EMAGE:7905 EMAGE:7905
euxassay_013010_20 euxassay_013010_21 euxassay_013010_22 euxassay_013010_23 euxassay_013010_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7905Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7905_wholemount_strong.wlz
7905_wholemount_moderate.wlz
7905_wholemount_weak.wlz
7905_wholemount_possible.wlz
7905_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7905_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39112
Entity Detected:Olfr1494, olfactory receptor 1494 ( MGI:3031328)
Sequence:sense strand is shown

>T39112
ATCAGTCTGGGACCACTGAGTTTGTGTTCCGTGTCTTCACCACTGTCCCTGAATTCCAGGCTCTTCTCTT
CCTCCTCTTCCTCCTGCTGTATTTGATGATCCTCTGTGGTAATGCAGCCATCATCTGGGTAGTGTGCACA
CACAGTGCCCTGCACACACCTATGTATTTCTTCCTCAGTAGTCTGTCTGTCTTGGAAATCTTCTACACCA
CAGATGTGGTGCCCTTGATGCTTTCTAACATCTTTGGGGCCCAGAAACCCATATCACTGGCTGGTTGTGG
GACACAAATGTTCTTCTTTGTAACTCTAGGAAGCACTGACTGCTTTCTCTTGGCAGTCATGGCCTATGAC
AGGTATGTGGCCATCTGCCACCCTCTACACTACAGCCTCATCATGACCAAGAAGCTGTGTGTCCAGATGG
TGATGGGCTCTTGGAGCTTGGCACTGTTTCTCTCCCTGCAGCTCACTGCCTTAATTTTCACCTTGCCCTT
CTGTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 152542. Forward Primer - name:152542_F_cDNA_Olfr1494, sequence:ATCAGTCTGGGACCACTGAGTT; Reverse Primer - name:152542_N_SP6_cDNA_Olfr1494, sequence:CACAGAAGGGCAAGGTGAAAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7904 same embryo
 EMAGE:7903 same embryo
 EMAGE:7906 same embryo
 EurExpress:euxassay_013010 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS