EMAGE mouse gene expression spatial database

EMAGE:7936

Olfr720 olfactory receptor 720 ( MGI:3030554)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013083. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013083_01	euxassay_013083_02	euxassay_013083_03	euxassay_013083_04

euxassay_013083_05	euxassay_013083_06	euxassay_013083_07	euxassay_013083_08	euxassay_013083_09

euxassay_013083_10	euxassay_013083_11	euxassay_013083_12	euxassay_013083_13	euxassay_013083_14

euxassay_013083_15	euxassay_013083_16	euxassay_013083_17	euxassay_013083_18	euxassay_013083_19

euxassay_013083_20	euxassay_013083_21	euxassay_013083_22	euxassay_013083_23	euxassay_013083_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

Download individual expression domains:

Download all expression domains:

Find spatially similar wholemount expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

single cell

moderate expression: see section 09 10 11 12 13 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39283
Entity Detected:	Olfr720, olfactory receptor 720 ( MGI:3030554)
Sequence:	sense strand is shown >T39283 TCTCACTCCCATCACCATGAGTCTCCCATTCTGTGCATCCCACAAGATTAATCACTTTTTCTGCGAGGCT CCCACAATGCTGAGGCTGGCCTGTGGTGACAAAGCCATATATGAAATGGTGATGTATATCTGCTGTGTTG TAATGCTGTTGGTCCCCTTCTCTGTGGTGATCACATCTTATGCCCAGATACTGGTCACAGTACATCACAT AAAGTCTGAAGAAGGGAGGAAGAAGGCTTTTGCCACCTGCTCATCACATGTGATTGTGGTGACATTGTTT TATGGGGCTGCTTTGTACACCTATAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 279234. Forward Primer - name:279234_F_cDNA_Olfr720, sequence:TCTCACTCCCATCACCATGA; Reverse Primer - name:279234_N_SP6_cDNA_Olfr720, sequence:ATATAGGTGTACAAAGCAGCCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7937 same embryo
	EMAGE:7934 same embryo
	EMAGE:7938 same embryo
	EMAGE:7935 same embryo
	EMAGE:7933 same embryo
	EurExpress:euxassay_013083 same experiment
	MGI:4826954 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source