EMAGE mouse gene expression spatial database

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EMAGE:7962

BC005561 cDNA sequence BC005561 ( MGI:3040669)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013130. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013130_01	euxassay_013130_02	euxassay_013130_03	euxassay_013130_04

euxassay_013130_05	euxassay_013130_06	euxassay_013130_07	euxassay_013130_08	euxassay_013130_09

euxassay_013130_10	euxassay_013130_11	euxassay_013130_12	euxassay_013130_13	euxassay_013130_14

euxassay_013130_15	euxassay_013130_16	euxassay_013130_17	euxassay_013130_18	euxassay_013130_19

euxassay_013130_20	euxassay_013130_21	euxassay_013130_22	euxassay_013130_23	euxassay_013130_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7962_wholemount_strong.wlz

7962_wholemount_moderate.wlz

7962_wholemount_weak.wlz

7962_wholemount_possible.wlz

7962_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7962_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38477
Entity Detected:	BC005561, cDNA sequence BC005561 ( MGI:3040669)
Sequence:	sense strand is shown >T38477 GTTTCTGGCTCCTTGAAATCACCTATTTCCCGAACAGATATCACAGAACCTGAAAGAGAAAAACGTCGCA AAGTTGATTCCCATCCTTCTCCATCACACTCTTCCACAATAAAGGACAGTCTTGTCAAACTTAAGGAATC TTCAGCAAAGCTCTATATCAACCATATTCCACCACTACTGTGCAAGAGTAAAGAGAGAGAAGCAGACAAG AAAGATTTGGACAAGTCAAGGGAAAGATCCAGAGAAAGAGAGAAAAAAGAGGAAAAGGACAGGAAAGAGC GGAAAAGAGATTATTCAAACAATGACCGAGAGGCACCCTTGGACTTAATCAAGAGGCGGAAAGATGAAAA TGGAATATTGGGGGTTTCAAAACACAAAAGTGAAAGTCCCTGTGAGTCTCTTTATCCAAATGAGAAAGAC AAGGAAAAAATGAAGTCAAAATCCTCAGGCAAAGAAAAAGGTGATTCATTTAAACCTGAAAAGATAGATA AAATATCCAGTGGGAAAAAGGAGTCCGGACATGATAAGGAAAAGATAGAGAAGAAAGAGAAATGGGATGG TTCTGGAGATAAGGAAGAGAAGAAACATCATAAAACCTCAGACAAGCACAGATAATGAAGACTCAGCCAA AAGTGTGGGCAGGCCTCTGAGCTGAAGGTCTCCCCTGCTGAGGATGCCAGAGCTCTTGGTGTCACTCTCT AAAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 149336. Forward Primer - name:149336_F_cDNA_BC005561, sequence:GTTTCTGGCTCCTTGAAATCAC; Reverse Primer - name:149336_N_SP6_cDNA_BC005561, sequence:GTTTAGAGAGTGACACCAAGAGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7959 same embryo
	EMAGE:7961 same embryo
	EMAGE:7960 same embryo
	EMAGE:7963 same embryo
	EMAGE:7964 same embryo
	EurExpress:euxassay_013130 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source