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EMAGE:8019

AU022751 expressed sequence AU022751 ( MGI:2147968)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8019 EMAGE:8019 EMAGE:8019 EMAGE:8019 EMAGE:8019
"Pseudo-wholemount" of euxassay_015969. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_015969_01 euxassay_015969_02 euxassay_015969_03 euxassay_015969_04
EMAGE:8019 EMAGE:8019 EMAGE:8019 EMAGE:8019 EMAGE:8019
euxassay_015969_05 euxassay_015969_06 euxassay_015969_07 euxassay_015969_08 euxassay_015969_09
EMAGE:8019 EMAGE:8019 EMAGE:8019 EMAGE:8019 EMAGE:8019
euxassay_015969_10 euxassay_015969_11 euxassay_015969_12 euxassay_015969_13 euxassay_015969_14
EMAGE:8019 EMAGE:8019 EMAGE:8019 EMAGE:8019 EMAGE:8019
euxassay_015969_15 euxassay_015969_16 euxassay_015969_17 euxassay_015969_18 euxassay_015969_19
EMAGE:8019 EMAGE:8019 EMAGE:8019 EMAGE:8019 EMAGE:8019
euxassay_015969_20 euxassay_015969_21 euxassay_015969_22 euxassay_015969_23 euxassay_015969_24
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euxassay_015969_25 euxassay_015969_26

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8019Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8019_wholemount_strong.wlz
8019_wholemount_moderate.wlz
8019_wholemount_weak.wlz
8019_wholemount_possible.wlz
8019_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8019_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
single cellmoderate expression: see section 01 02 03 04 05 06 07 08 09 18 19 22 23 24 25 weak expression: see section 20 21
axial skeleton
moderate moderate
single cellmoderate expression: see section 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39450
Entity Detected:AU022751, expressed sequence AU022751 ( MGI:2147968)
Sequence:sense strand is shown

>T39450
CCAGAGTCTTCTCTGAGCCCTAGGCCTGACCAAAGCCACCATTTTGATTTTCCAGTGGGGAACCTAGAAG
CCCCGGGGCCTACCCTCAGGAGCTCCACCTCACAGGGCTCGGGGTCTACTCCAGTCCCCGAGGCCCTCCG
CTGTGCCGAGTCCAGCCGTGCCGAGAGTGACCAGTCCAGCCCTGCTGGCCGAGAACTGCGTCAACAGGCC
AGTCCACGGGCTCCCGATGATGATGATGACGGTGATGGTGGTCCTGATCCCAGAGGCAGCGGGACCCCCG
AAGGCTGGGTCCTCCGTTCCGGAGTTGTACCTTTCGGTCGTAGGAGCAGTGCATCGGAGGTCAGCCCCGA
AGAGGTTCGCCCCGAAGCCCAGTGCACAGGCTGGAATTTACGTCCTCGTCCTCGGAGCAGTGCATCAGCG
GTCAGCCCTGAAGCTCGCCCCAAAGCCCAGAGCGCAGGCAGGAATTTACGTCCTCGTCCTCGGAGCAGTG
CATCAGTGGTCAGCCCTGAAGCTCGCCCCAAAGCCCAGAGCGCAGGCAGGAATTTACGTCCTCGTCCTCG
GAGCAGTGCATCAGTGGTCAGCCCCGAAGCTCGCCCGGAAGCCCAGAGCGCAGGCAGGAATTTACGTCCT
CGGGCCACTCCACGAGTTCCTGTTGCTCCCAGCAGTACCACGAGGTCCAGTTCTGATAGAGGCAGCAGCC
GTGCCCCACGGAGCCGGAGCCGAAGCCGCAGCTGCAGTACACCCAGGCTCGGCTCTGACCATCAACGGAG
CCGTAAAATAAAGATGAGGCTGGATCTGCAAGTGGACCGTGAGCCGGAATCGGAGGCAGAGCAAGAAGAG
CAAGAGCTGGAGTCAGAACCTGGCCCTAGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 111652. Forward Primer - name:111652_F_cDNA_LOC208429, sequence:CCAGAGTCTTCTCTGAGCCCTA; Reverse Primer - name:111652_N_SP6_cDNA_LOC208429, sequence:GCTAGGGCCAGGTTCTGACTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8022 same embryo
 EMAGE:8020 same embryo
 EMAGE:8018 same embryo
 EMAGE:8021 same embryo
 EMAGE:8023 same embryo
 EurExpress:euxassay_015969 same experiment
 MGI:4823353 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS