EMAGE mouse gene expression spatial database

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EMAGE:8171

Tox4 TOX high mobility group box family member 4 ( MGI:1915389)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016419. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016419_01	euxassay_016419_02	euxassay_016419_03	euxassay_016419_04

euxassay_016419_05	euxassay_016419_06	euxassay_016419_07	euxassay_016419_08	euxassay_016419_09

euxassay_016419_10	euxassay_016419_11	euxassay_016419_12	euxassay_016419_13	euxassay_016419_14

euxassay_016419_15	euxassay_016419_16	euxassay_016419_17	euxassay_016419_18	euxassay_016419_19

euxassay_016419_20	euxassay_016419_21	euxassay_016419_22	euxassay_016419_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8171_wholemount_strong.wlz

8171_wholemount_moderate.wlz

8171_wholemount_weak.wlz

8171_wholemount_possible.wlz

8171_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8171_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

glossopharyngeal ix ganglion

weak

single cell

weak expression: see section 06 07 17

trigeminal v ganglion

weak

single cell

weak expression: see section 05 06 07 08 16 17 18 21 22

ventral grey horn

moderate

single cell

moderate expression: see section 10 11 13 14 15 16

dorsal root ganglion

moderate

single cell

moderate expression: see section 15 16 17 weak expression: see section 07 08 09 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63216
Entity Detected:	Tox4, TOX high mobility group box family member 4 ( MGI:1915389)
Sequence:	sense strand is shown >T63216 TGGGGTTATGGCTTAGCACTATAAAGCTCTGAGTTCAGACCCCAGGCTGGAAGATCAGGAGTTCAAGGTC ATCCTTGGCCCACTAGTGAGTTTGAGAACAGTCTAAGACGCATGAGGTAAAGGTTTTACAGAACATGGTA GCCCGTGCCTTTAATCCTAATACTTTGGAAGTAGACATAGGAAGATTTTTGAGTAAGGCCAATCTGAACT TAAATCAGACTTTGTCTCAAAAAACGGACCGGGTGTGGTGGCACTCCCCTTTAATCCTACAACTTGGGAG GCAAGAGGCAGGTTGGATCTCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 141950. Forward Primer - name:141950_F_cDNA_5730589K01Rik, sequence:TGGGGTTATGGCTTAGCACTAT; Reverse Primer - name:141950_N_SP6_cDNA_5730589K01Rik, sequence:AGAGATCCAACCTGCCTCTTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8168 same embryo
	EMAGE:8172 same embryo
	EMAGE:8169 same embryo
	EMAGE:8167 same embryo
	EMAGE:8170 same embryo
	EurExpress:euxassay_016419 same experiment
	MGI:4828873 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source