EMAGE mouse gene expression spatial database

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EMAGE:8187

Trpc4ap transient receptor potential cation channel, subfamily C, member 4 associated protein ( MGI:1930751)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007528. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007528_01	euxassay_007528_02	euxassay_007528_03	euxassay_007528_04

euxassay_007528_05	euxassay_007528_06	euxassay_007528_07	euxassay_007528_08	euxassay_007528_09

euxassay_007528_10	euxassay_007528_11	euxassay_007528_12	euxassay_007528_13	euxassay_007528_14

euxassay_007528_15	euxassay_007528_16	euxassay_007528_17	euxassay_007528_18	euxassay_007528_19

euxassay_007528_20	euxassay_007528_21	euxassay_007528_22	euxassay_007528_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8187_wholemount_strong.wlz

8187_wholemount_moderate.wlz

8187_wholemount_weak.wlz

8187_wholemount_possible.wlz

8187_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8187_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9049
Entity Detected:	Trpc4ap, transient receptor potential cation channel, subfamily C, member 4 associated protein ( MGI:1930751)
Sequence:	sense strand is shown >T9049 GGGACCGCTTGCTTCCTGTTTGTCGGACGAGGAGACATGGCGGCAGCGCCCGCAGCGGCTGGAGCTGGGG CCAGCCGGGGGAGGCGGTTGGCAGCTACCGCGGCGGCGTGGGGCGGCTGGGGAGGTCGACCGCGGCCGGG TAACATCCTGCTGCAGCTGCGGCAGGGCCAGCTCACCGGCCGAGGCCTGGTCCGGGCGGTGCAGTTCACT GAGACCTTTTTGACCGAGAGGGACAAATTGTCCAAATGGAGTGGAATTCCTCAGTTGCTCCTTAAGCTGT ATGCCACCAGCCATCTGCACAGTGACTTCGTTGAGTGCCAAAGCATTCTCAAGGAGATTTCTCCTCTTCT CTCTATGGAGGCTATGGCGTTTGTAACTGAAGACAGGAAATTTACTCAAGAAGCTACATACCCAAATACA TATATTTTTGACTTATTTGGAGGTGTTGATCTTCTTGTAGAAATTCTTATGAGGCCTACCATCTCTATCC GGGGACAGAAACTAAAAATAAGTGACGAGATGTCGAAGGACTGCTTGAGCATCCTGTACAATACCTGTGT CTGCACGGAAGGAGTAACGAAGCGTTTGGCAGAAAAGAATGATTTTGTGATTTTTCTGTTCACACTGATG ACAAGTAAGAAGACATTCTTACAAACAGCGACTCTCATTGAAGACATTTTGGGTGTTAAGAAGGAAATGA TCCGGCTAGATGAAGTTCCCAATCTGAGTTCCTTAGTATCCAATTTCGATCAGCAGCAGCTTGCTAACTT CTGCAGGATCCTGGCTGTCACCATTTCAGAGATGGACACGGGGAATGACGACAAACACACGCTTCTTGCC AAAAATGCTCAGCAGAAGAAGAGTTTGAGTTTGGGGCCTTCTGCAGCTGAAATCAACCAAGCTGCTCTTC TCAGCATCCCTGGCTTTGTGGAACGGCTTTGCAAACTGGCAACTAGGAAAGTGTCAGAGTCAACGGGCAC AGCCAGCTTCCTTCAAGAACTGGAGGAGTGGTACACATGGCTCGACAATGCGTTGGTGCTAGATGCCCTG ATGCGAGTGGCCAATGAGGAGTCTGAACACAATCAAGCCCCCACTGTGTTCCCTTCTCTAGGCACTTCGG AGGAGGGTGGGCTGCCACACACCTCGGCCAGGGCGCAGCTGCCCCAGTCCATGAAGATTATGCATGAGAT TATGTACAAACTGGAAGTGCTTTATGTCCTCTGCGTGCTGCTGATGGGGCGTCAGCGAAACCAGGTCCAC AGGATGATTGCTGAGTTCAAGTTGATCCCAGGACTCAATAATTTGTTCGACAAACTGATTTGGAGGAAGC ATTCAGCATCTGCCCTTGTCCTCCATGGCCACAACCAGAACTGTGACTGTAGCCCGGACATCACCTTGAA GATCCAGTTTTTGAGACTTCTCCAGAGTTTCAGTGACCACCATGAGAACAAGTACTTGCTGCTCAACAAC CAGGAGCTAAATGAGCTCAGTGCCATCTCGCTGAAAGCCAACATCCCTGAGGTGGAGGCTGTCCTCAACA CTGACAGGAGTCTGGTGTGTGACGGCAAGAGGGGCCTACTAACTCGGCTGCTGCAGGTCATGAAGAAGGA GCCAGCGGAGTCTTCATTCAGGTTTTGGCAAGCCCGGGCTGTGGAAAGTTTCCTCCGAGGGACCACCTCC TATGCAGACCAGATGTTCCTGCTGAAGCGAGGCCTTCTGGAGCACATCTTATACTGCATTGTGGACAGTG AGTGCAAGTCTAGGGATGTACTCCAGAGCTACTTTGATCTCCTGGGAGAGCTGATGAAGTTCAACGTCGA TGCATTCAAGAGATTCAATAAATATATCAACACTGATGCAAAGTTCCAGGTGTTCCTGAAACAGATCAAC AGCTCGCTAGTGGACTCCAACATGCTGGTGCGCTGTGTCACTCTGTCACTGGACCGATTTGAAAACCAGG TGGACATGAAAGTGGCCGAGGTCCTGTCCGAGTGCCGCCTGCTTGCCTACATATCCCAGGTGCCCACGCA AATGTCCTTCCTCTTCCGCCTCATCAATATCATCCATGTGCAGACGCTAACCCAGGAGAACGTCAGCTGC CTCAACACCAGCCTGGTAATCCTGATGCTGGCCCGACGGAAAGAGCGGCTGCCCCTGTACCTGCGACTGC TACAGCGAATGGAGCACAGCAAGAAATACCCCGGCTTCCTGCTCAACAACTTCCACAACCTGCTGCGCTT TTGGCAGCAGCACTACCTGCACAAGGATAAGGACAGCACCTGCCTGGAGAATAGCTCCTGTATCAGCTTC TCGTACTGGAAGGAGACAGTGTCCATCTTGTTGAACCCAGACCGCCAGTCGCCCTCTGCCCTCGTCAGCT ACATTGAGGAGCCCTACATGGACATAGACAGAGACTTCACTGAGGAGTGACCTTGAGCCAGGCTTCAAGA AGCTGCCAGGCAGGGGCGAGTGCAGAGAGCCAGAGTGCACGTGCCGCCTCGCACTCTGCCCATCCCTGTC CCTCTTGCCTCCCCAGTGCTCTGTCCACCCCGGGTGTCCAGGTGCAGGCTCAGTGGAAGGAACATCCTAG AAGCTCTTGTCTCCTGGGTCTGAGGGCCCAGGTCATGGGGAGCCTGTTTCTTCACTGTCCCCAGGGTAAG GACAATGGCACCATGCCCACCTTTCCCTCGCAATCCTGGAGCCCTGGCCTGCCAGAGAAGGGGAACACGG GAAACCATCTGTCTGAGCTTCTCCCATCAGTCCTGATGGAGAGGAAGATGCAGACCCCTGTGAGCCAAAC CTCCCTGGCCTCAGGACGCAGGCAGAGAGGCTCCTGAGCCCTGGGCAGGGGTGGGTATCGGAGAATGCTG CCTTCCCCGGGGTCTGCCCATCTCTCCCTTCCTCACCTCCCTTGTCTTCCTTTAGCTCCTCTGGTTCTTT GCTCATTGGCCACTGTGTTCATCCAGGAGGCTCCCTAGAAGTGGGGGGAGCCTTTTCTCTCCATCCCTTG GGGCATGGAGTGCCTGTGCCTGCCTGAGCCCAGACACGGGAGCCTGGCTGAGAGCTTACCCTTGGCTCCT CCTGTGTTGCCAGTTTATTAACAGCAAATAAACCGATTAAATGGAGACTATTAAAGAACTTTATTTTAAA GACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6816155 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6816155 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007528 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source