EMAGE mouse gene expression spatial database

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EMAGE:8238

1700001L19Rik RIKEN cDNA 1700001L19 gene ( MGI:1916565)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007556. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007556_01	euxassay_007556_02	euxassay_007556_03	euxassay_007556_04

euxassay_007556_05	euxassay_007556_06	euxassay_007556_07	euxassay_007556_08	euxassay_007556_09

euxassay_007556_10	euxassay_007556_11	euxassay_007556_12	euxassay_007556_13	euxassay_007556_14

euxassay_007556_15	euxassay_007556_16	euxassay_007556_17	euxassay_007556_18	euxassay_007556_19

euxassay_007556_20	euxassay_007556_21	euxassay_007556_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8238_wholemount_strong.wlz

8238_wholemount_moderate.wlz

8238_wholemount_weak.wlz

8238_wholemount_possible.wlz

8238_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8238_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

facial vii ganglion

weak

regional

weak expression: see section 03 04 06 17 18 19 20

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 16 17

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 07 16 17 18 19 20 21

vagus x ganglion

weak

regional

weak expression: see section 06 07 16

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 05 06 07 16 17 18 19

spinal cord

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 15 16 17 weak expression: see section 06 07 08 09 10

neural retina

weak

regional

weak expression: see section 01 02 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35361
Entity Detected:	1700001L19Rik, RIKEN cDNA 1700001L19 gene ( MGI:1916565)
Sequence:	sense strand is shown >T35361 GGAAAAGAAGAGGGAAGAGAGGAGGAGGGGGGAGAGGAGGAGGAAGTCACCTCCGAAACCCTGCGTGGCA AACCCCGACCTCTGCCCATCTCAGCACTACCAGCTTTCAGCTACATCCCACCAAGGCACCAGGGCCCCAA GGAGCGCAGCTACTTCAGTCGCGAGGGCCAGACAGGGATTGTCTCCCTCTATGACTGTGTTTTTAAGAGG AGACTAGATTATAACCAGAAATTACACCGAGATGACAGAGAACATGCAAAGAACCTGGGGCTCCATATTA ATGAGGAGGAACAAGAAAGGACTGTGCCAGTGCTGATGTCCTCTGTCTATGGGAAACGCATCAATCAGCC CATTGAGCCCCTAAACAGAGACTATGGCCATGTGAGCCACGTGAAGACCGACTTCTACAGGAAGAACGAG ATCCCCAGCATCAAGGGGCCCGGCTTTGGGCACATCAATCCAGCCTGAAGAAGTCGTGTGGTTTCTAGGG CACTCTGAGGTACCCTATCAGCAAGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 84391. Forward Primer - name:084391_F_cDNA_1700001L19Rik, sequence:GGAAAAGAAGAGGGAAGAGAGG; Reverse Primer - name:084391_N_SP6_cDNA_1700001L19Rik, sequence:CACTTGCTGATAGGGTACCTCAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8241 same embryo
	EMAGE:8240 same embryo
	EMAGE:8239 same embryo
	EurExpress:euxassay_007556 same experiment
	MGI:4822618 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source