EMAGE mouse gene expression spatial database

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EMAGE:8334

Padi3 peptidyl arginine deiminase, type III ( MGI:1338891)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007682. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007682_01	euxassay_007682_02	euxassay_007682_03	euxassay_007682_04

euxassay_007682_05	euxassay_007682_06	euxassay_007682_07	euxassay_007682_08	euxassay_007682_09

euxassay_007682_10	euxassay_007682_11	euxassay_007682_12	euxassay_007682_13	euxassay_007682_14

euxassay_007682_15	euxassay_007682_16	euxassay_007682_17	euxassay_007682_18	euxassay_007682_19

euxassay_007682_20	euxassay_007682_21	euxassay_007682_22	euxassay_007682_23	euxassay_007682_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8334_wholemount_strong.wlz

8334_wholemount_moderate.wlz

8334_wholemount_weak.wlz

8334_wholemount_possible.wlz

8334_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8334_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 04 19 20 moderate expression: see section 05

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 18

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 16 17 18 19 20 21 22

vagus x ganglion

weak

regional

weak expression: see section 07 17

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 weak expression: see section 09 10 11 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36736
Entity Detected:	Padi3, peptidyl arginine deiminase, type III ( MGI:1338891)
Sequence:	sense strand is shown >T36736 CCTCAGAAGCCTTTTCTCTTGAAATGTCTGCCTTTATCTGCCACTCATTCAGATCCCAGACGTGAATCCT CTTGGTGTCCCCAAGAAAGGGCCCTGTGTCTACCAGACTCTCATGGGGATCAACGTGACCCATGGCGATA GCTTTAAACACCATCCCCACAAACTCCTCTGTTTGTGGGTAAATCCACTCGGATGTCTAGAGGCTTTGAC CTTGTCAGCATCTTGGCCTGCACTTCCTATGGTGGAAGGCCCAGGGGTTTGGTTTTGGCCACTGTCTCCT GCCCTGCAGGGGATGGTTTTAAAGTCAGACAATTTTACATATTCTTGTACGAGTAGACTACTCCACATGA GAGAGTCTACCCCAGGAAGCACCCCAGGGTCCATTCAAGTCCAAGTGTGACCATCATTCTTACTGCAAGC TCCCGGCCAGGACCGGCCTTTACTGAGCTGTCACACAGTGGGTACCTCTAGGTGCTTTTGGGATGGCAGG TATCTCTTAGCCTTACAAGCTCCCCATCATCTGATGCCCAGGAATCGGGTTGTCCAAATCCTCTTGAAAT TTCTCTGAAGTGAAGCGGCTGGGGGCAGGGGGAAGGGGGGCAGCGCTGTTGATGGTTAGGAAAACTGGAT TCCCAGAGAAATCTAAGACAGGACCGACCCTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 99309. Forward Primer - name:099309_F_cDNA_Padi3, sequence:CCTCAGAAGCCTTTTCTCTTGA; Reverse Primer - name:099309_N_SP6_cDNA_Padi3, sequence:CAGGGTCGGTCCTGTCTTAGAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8331 same embryo
	EMAGE:8333 same embryo
	EMAGE:8332 same embryo
	EurExpress:euxassay_007682 same experiment
	MGI:4827024 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source