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EMAGE:8363

Pklr pyruvate kinase liver and red blood cell ( MGI:97604)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8363 EMAGE:8363 EMAGE:8363 EMAGE:8363 EMAGE:8363
"Pseudo-wholemount" of euxassay_009487. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009487_01 euxassay_009487_02 euxassay_009487_03 euxassay_009487_04
EMAGE:8363 EMAGE:8363 EMAGE:8363 EMAGE:8363 EMAGE:8363
euxassay_009487_05 euxassay_009487_06 euxassay_009487_07 euxassay_009487_08 euxassay_009487_09
EMAGE:8363 EMAGE:8363 EMAGE:8363 EMAGE:8363 EMAGE:8363
euxassay_009487_10 euxassay_009487_11 euxassay_009487_12 euxassay_009487_13 euxassay_009487_14
EMAGE:8363 EMAGE:8363 EMAGE:8363 EMAGE:8363 EMAGE:8363
euxassay_009487_15 euxassay_009487_16 euxassay_009487_17 euxassay_009487_18 euxassay_009487_19
EMAGE:8363 EMAGE:8363
euxassay_009487_20 euxassay_009487_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8363Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8363_wholemount_strong.wlz
8363_wholemount_moderate.wlz
8363_wholemount_weak.wlz
8363_wholemount_possible.wlz
8363_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8363_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 06 07 15 16
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 08 11 12
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 08 11 weak expression: see section 12
liver lobe
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36796
Entity Detected:Pklr, pyruvate kinase liver and red blood cell ( MGI:97604)
Sequence:sense strand is shown

>T36796
CAAAGACAGTGTGGGTGGACTACCACAATATCACCCAGGTCGTTGCAGTGGGGGGCCGCATCTACATTGA
CGACGGGCTCATCTCCTTAGTGGTGCGGAAAATTGGCCCAGAGGGACTGGTGACCGAAGTGGAACACGGT
GGTTTCTTGGGCAACAGGAAGGGTGTGAACTTGCCAAATGCCGAGGTGGACCTGCCTGGGCTATCAGAGC
AAGATCTTTTGGATCTGCGCTTCGGGGTGGAGCATTATGTGGACATCATCTTTGCCTCCTTTGTACGAAA
AGCCAGTGATGTGGTGGCAGTCCGAGATGCCCTAGGGCCAGAAGGACGGGGCATCAAAATTATCAGCAAA
ATCGAGAACCATGAAGGCGTGAAGAAGTTTGATGAGATCCTAGAAGTGAGCGATGGCATCATGATGGCTC
GGGGTGACCTTGGCATTGAGATCCCAGCAGAGAAGGTTTTCTTGGCTCAGAAGATGATGATTGGACGCTG
CAACCTGGCTGGCAAGCCTGTCGTTTGTGCCACACAGATGCTGGAGAGCATGATCACTAAGGCTCGACCA
ACTCGGGCGGAGACAAGCGATGTGGCCAATGCTGTGCTGGATGGGGCTGACTGTATCATGCTGTCTGGAG
AGACC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74692. Forward Primer - name:074692_F_cDNA_Pklr, sequence:CAAAGACAGTGTGGGTGGACTA; Reverse Primer - name:074692_N_SP6_cDNA_Pklr, sequence:GGTCTCTCCAGACAGCATGATA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8360 same embryo
 EMAGE:8359 same embryo
 EMAGE:8362 same embryo
 EMAGE:8358 same embryo
 EMAGE:8361 same embryo
 EurExpress:euxassay_009487 same experiment
 MGI:4827232 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS