EMAGE mouse gene expression spatial database

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EMAGE:8551

Nup43 nucleoporin 43 ( MGI:1917162)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007275. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007275_01	euxassay_007275_02	euxassay_007275_03	euxassay_007275_04

euxassay_007275_05	euxassay_007275_06	euxassay_007275_07	euxassay_007275_08	euxassay_007275_09

euxassay_007275_10	euxassay_007275_11	euxassay_007275_12	euxassay_007275_13	euxassay_007275_14

euxassay_007275_15	euxassay_007275_16	euxassay_007275_17	euxassay_007275_18	euxassay_007275_19

euxassay_007275_20	euxassay_007275_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8551_wholemount_strong.wlz

8551_wholemount_moderate.wlz

8551_wholemount_weak.wlz

8551_wholemount_possible.wlz

8551_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8551_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

spotted

strong expression: see section 01 02 03 04 05 06 08 09 10 13 14 15 16 17 18 19 moderate expression: see section 07 11 12

pectoral girdle and thoracic body wall musculature

moderate

regional

moderate expression: see section 04 05 06 07

thymus primordium

weak

regional

weak expression: see section 12 13 14 15

nasal cavity olfactory epithelium

strong

spotted

strong expression: see section 09 10 16 17

midgut

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 15

lower jaw molar

moderate

regional

moderate expression: see section 10 18

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15

upper jaw molar

moderate

regional

moderate expression: see section 10 18

liver

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

bladder

weak

regional

weak expression: see section 13 14

renal cortex

strong

spotted

strong expression: see section 08 09 10 11 17 18 moderate expression: see section 12 19

left lung

strong

spotted

strong expression: see section 03 04 05 06 07 09 10 11 12 moderate expression: see section 08

right lung

strong

spotted

strong expression: see section 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1827
Entity Detected:	Nup43, nucleoporin 43 ( MGI:1917162)
Sequence:	sense strand is shown >T1827 GGCATGGAGGAAATTTATGCTAAGTTCGTGTCTCAGAAAATTAGCAAAACCCGCTGGCGGCCAGTGCCTT CCGGGAGCCTGCAGACCACAGAGACCTTTGCCACGGGCTCTTGGGACAATGAGGAAAATTGCGTTTCACT GTGGTCTATTGGAGATTTTGGAAACTTGGATTCTGACGGAGGGTTTGAAGGAGACCATCAGTTACTGTGC GATATTAGACATCATGGGGATGTCATGGATTTACAGTTTTTTGACCAGGAAAGAATTGTAGCTGCTTCGT CAACAGGCTGTGTAACAGTTTTTCTTCACCATCCAAATAACCAGACCCTGTCCGTCAATCAGCAGTGGCC TGCAGCTCATTACCATACAGGTCCCAGCAGTCCCTCCTATAGCAGTGCACCATGTACAGGAATTGTGTGT GACAACCCAGAAATTGTTACAGTTGGAGAGGATGGCCGAATAAATCTCTTCAGAGTTGATCACAAGGAGG CTGTCAGAACTATAGATAATGCAGACAGCAGTACACTC
Notes:	The probe template was PCR amplified from IMAGE:577944 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:577944 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8553 same embryo
	EMAGE:8552 same embryo
	EurExpress:euxassay_007275 same experiment
	MGI:4826834 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source