EMAGE mouse gene expression spatial database

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EMAGE:8820

H2-Bl histocompatibility 2, blastocyst ( MGI:892004)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010046. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010046_01	euxassay_010046_02	euxassay_010046_03	euxassay_010046_04

euxassay_010046_05	euxassay_010046_06	euxassay_010046_07	euxassay_010046_08	euxassay_010046_09

euxassay_010046_10	euxassay_010046_11	euxassay_010046_12	euxassay_010046_13	euxassay_010046_14

euxassay_010046_15	euxassay_010046_16	euxassay_010046_17	euxassay_010046_18	euxassay_010046_19

euxassay_010046_20	euxassay_010046_21	euxassay_010046_22	euxassay_010046_23	euxassay_010046_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8820_wholemount_strong.wlz

8820_wholemount_moderate.wlz

8820_wholemount_weak.wlz

8820_wholemount_possible.wlz

8820_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8820_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 11 14 15 moderate expression: see section 12 13

hindgut

moderate

regional

moderate expression: see section 13 14

rectum

moderate

regional

moderate expression: see section 13 14

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 weak expression: see section 06

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 21 moderate expression: see section 19 20 22 23 weak expression: see section 24

lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31793
Entity Detected:	H2-Bl, histocompatibility 2, blastocyst ( MGI:892004)
Sequence:	sense strand is shown >T31793 GAGCCCCGGTTCATCTCTGTCGGCTACGTGGACAACACGCAGTTCGTGAGCTTCGACAGCGACGCGGAGA ATCCGAGATCTGAGCCGCGGTCGCCCTGGATGGAGCAGGAGGGACCGGAGTATTGGGAGCGGGAAACACA GATCGCCAAGGACAATGAGCAGAGTTTCGGATGGAGCCTGAGGAACCTGATCCACTACTACAACCAGAGC AAGGGTGGCTTTCACACATTCCAGCGGTTGTCTGGCTGTGACATGGGGTTGGACGGGCGCCTCCTCCGCG GGTACCTGCAGTTCGCTTATGATGGCCGCGATTACATCGCCCTGAACGAAGACCTGAAAACGTGGATGGC GGCGGACCTGGTAGCGCTGATCACCCGACGCAAGTGGGAGCAGGCTGGTGCTGCAGAGCTATACAAGTTC TACCTAGAGGGCGAATGCGTGGAGTGGCTTCGCAGATACCTGGAGCTCGGGAATGAGACGCTGCTGCGCA CAGATCCCCCAAAGGCCCATGTGACCCGTCATCCCAGACCTGCAGGTGATGTCACCCTGAGGTGCTGGGC CCTGGGCTTCTACCCTGCTGACATCACCCTGACCTGGCAGTTGAATGGGGAGGAGCTGACCCAGGACATG GAGCTTGTGGAGACCAGGCCTGCAGGGGATGGAACCTTCCAGAAGTGGGCAGCTGTGGTGGTGCCTCTTG GGAAAGAGCAGAATTATACATGCCGTGTGCACCATGAGGGGCTGCCTGAGCCACTCACCCTGAGATGGGA GCCTCCTCCATCAACCGGCTCCAACATGGTAAACATAGCTGTTCTGGTTGTCCTTGGAGCTGTGATCATC ATTGAAGCTATGGTGGCTTTTGCGTTGAAGAGCAGGAGAAAAATAGCCATCCTTCCTGGTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5026047), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60296. Forward Primer - name:060296_F_IRAV98_b05_H2-Bl, sequence:GAGCCCCGGTTCATCTCT; Reverse Primer - name:060296_R_SP6_IRAV98_b05_H2-Bl, sequence:GGACCAGGAAGGATGGCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8816 same embryo
	EMAGE:8815 same embryo
	EMAGE:8818 same embryo
	EMAGE:8819 same embryo
	EMAGE:8817 same embryo
	EurExpress:euxassay_010046 same experiment
	MGI:4825305 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source