Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:9035

Trim72 tripartite motif-containing 72 ( MGI:3612190)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9035 EMAGE:9035 EMAGE:9035 EMAGE:9035 EMAGE:9035
"Pseudo-wholemount" of euxassay_012915. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012915_01 euxassay_012915_02 euxassay_012915_03 euxassay_012915_04
EMAGE:9035 EMAGE:9035 EMAGE:9035 EMAGE:9035 EMAGE:9035
euxassay_012915_05 euxassay_012915_06 euxassay_012915_07 euxassay_012915_08 euxassay_012915_09
EMAGE:9035 EMAGE:9035 EMAGE:9035 EMAGE:9035 EMAGE:9035
euxassay_012915_10 euxassay_012915_11 euxassay_012915_12 euxassay_012915_13 euxassay_012915_14
EMAGE:9035 EMAGE:9035 EMAGE:9035 EMAGE:9035 EMAGE:9035
euxassay_012915_15 euxassay_012915_16 euxassay_012915_17 euxassay_012915_18 euxassay_012915_19
EMAGE:9035 EMAGE:9035 EMAGE:9035
euxassay_012915_20 euxassay_012915_21 euxassay_012915_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9035Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9035_wholemount_strong.wlz
9035_wholemount_moderate.wlz
9035_wholemount_weak.wlz
9035_wholemount_possible.wlz
9035_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9035_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38663
Entity Detected:Trim72, tripartite motif-containing 72 ( MGI:3612190)
Sequence:sense strand is shown

>T38663
AGCCACAGACAGAATTCCTCATGAAATTCTGCCTGGTAACCAGCAGGCTGCAGAAGATCCTGTCAGAGTC
ACCACCACCGGCAAGGCTAGATATCCAGCTGCCTGTCATCTCAGATGACTTCAAATTCCAGGTGTGGAAG
AAGATGTTCCGGGCTCTGATGCCAGCGCTGGAGGAACTGACTTTTGACCCCAGCTCTGCGCACCCGAGCC
TGGTGGTGTCCTCCTCTGGTCGCCGAGTGGAGTGCTCAGACCAGAAGGCGCCGCCAGCGGGAGAAGACAC
GCGTCAGTTCGACAAGGCAGTAGCGGTGGTGGCGCAGCAGCTGCTGTCACAGGGCGAGCACTATTGGGAG
GTGGAGGTGGGCGACAAACCACGCTGGGCCCTGGGAGTGATGGCGGCTGACGCTTCCCGCCGTGGCCGGC
TGCACGCGGTGCCCTCACAGGGGCTGTGGCTGCTGGGTCTGCGCGATGGCAAGATCCTGGAGGCGCACGT
GGAGGCCAAGGAGCCGCGGGCACTGCGCACCCCAGAGAGGCCTCCGGCGCGCATTGGCCTCTACCTAAGC
TTCGCAGATGGCGTCCTGGCTTTCTATGATGCGAGCAACCCCGACGTACTTACGCCAATCTTTTCTTTCC
ACGAGCGTCTGCCCGGGCCGGTGTACCCCATCTTTGACGTGTGCTGGCACGACAAGGGCAAGAATGCCCA
GCCCCTGCTGCTTGTGGGGCCGGAGCAGGAACAGGCCTGAGCTCCTGCCGACTGGGAGGGAGGCCTGGTC
CTGAAGGCGGTGGGATCACTCAGGCCACCTTTGTTAGGTCTGGATGGGTTGGGTGGGGGTGGATTTCTAG
GGCCTCACCCTCTGGCTGAGGGTGGCCAGGAAGTGGGTGTTTGTCTTA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 108607. Forward Primer - name:108607_F_cDNA_LOC434246, sequence:AGCCACAGACAGAATTCCTCAT; Reverse Primer - name:108607_N_SP6_cDNA_LOC434246, sequence:TAAGACAAACACCCACTTCCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9031 same embryo
 EMAGE:9030 same embryo
 EMAGE:9034 same embryo
 EMAGE:9032 same embryo
 EMAGE:9033 same embryo
 EurExpress:euxassay_012915 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS