Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:9308

Pex14 peroxisomal biogenesis factor 14 ( MGI:1927868)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9308 EMAGE:9308 EMAGE:9308 EMAGE:9308 EMAGE:9308
"Pseudo-wholemount" of euxassay_017939. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017939_01 euxassay_017939_02 euxassay_017939_03 euxassay_017939_04
EMAGE:9308 EMAGE:9308 EMAGE:9308 EMAGE:9308 EMAGE:9308
euxassay_017939_05 euxassay_017939_06 euxassay_017939_07 euxassay_017939_08 euxassay_017939_09
EMAGE:9308 EMAGE:9308 EMAGE:9308 EMAGE:9308 EMAGE:9308
euxassay_017939_10 euxassay_017939_11 euxassay_017939_12 euxassay_017939_13 euxassay_017939_14
EMAGE:9308 EMAGE:9308 EMAGE:9308 EMAGE:9308 EMAGE:9308
euxassay_017939_15 euxassay_017939_16 euxassay_017939_17 euxassay_017939_18 euxassay_017939_19
EMAGE:9308 EMAGE:9308 EMAGE:9308 EMAGE:9308 EMAGE:9308
euxassay_017939_20 euxassay_017939_21 euxassay_017939_22 euxassay_017939_23 euxassay_017939_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9308Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9308_wholemount_strong.wlz
9308_wholemount_moderate.wlz
9308_wholemount_weak.wlz
9308_wholemount_possible.wlz
9308_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9308_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50799
Entity Detected:Pex14, peroxisomal biogenesis factor 14 ( MGI:1927868)
Sequence:sense strand is shown

>T50799
GGAAGATGGCGTCGTCGGAGCAGGCAGAGCAGCCGAACCAGCCAAGCTCTCCTCCAGGAAGTGAAAATGT
GGTGCCCCGAGAGCCACTGATTGCCACAGCAGTGAAGTTCCTACAGAATTCTCGGGTCCGGCAGAGCCCA
CTTGCGACCAGGAGAGCATTTCTTAAGAAGAAAGGACTGACAGACGAAGAGATTGACCTGGCTTTCCAGC
AGTCGGGCACAGCTGCCGACGAGCCTTCACCCCTGGGCCCAGCTACCCCAGTGGTTCCTGTCCAGCCCCC
TCACCTCACTCCGCAGCCATACAGCCCCAGAGGCTCCCGATGGCGAGATTATGGTGCCTTGGCTATCATC
ATGGCAGGAATTGCATTTGGCTTTCACCAACTCTACAAGAGGTACCTGCTGCCTGCTGTCCTCTGTATCA
CACAAGACGTCTCGCTTGCTGTGTCGTGGTTTAGCTCCTCGGTGGTGAGGCGTGCTTCTCCCTCCTGGTG
GAGCCAGGCAGCAGGGCCTTCCTGACCAACAGCCATGTCTGACCTTCCCACTGAGAAACACATGTTCATT
TATGTGATCATGTATAGATTTCAGAATATAAGATATGACTGTACATAATTGTAATAAATACGAGTTGCCA
TATTTAAAAAAAAA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9309 same embryo
 EurExpress:euxassay_017939 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS