EMAGE mouse gene expression spatial database

EMAGE:9857

Mapk4 mitogen-activated protein kinase 4 ( MGI:2444559)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018744. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018744_01	euxassay_018744_02	euxassay_018744_03	euxassay_018744_04

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euxassay_018744_10	euxassay_018744_11	euxassay_018744_12	euxassay_018744_13	euxassay_018744_14

euxassay_018744_15	euxassay_018744_16	euxassay_018744_17	euxassay_018744_18	euxassay_018744_19

euxassay_018744_20	euxassay_018744_21	euxassay_018744_22	euxassay_018744_23	euxassay_018744_24

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50662
Entity Detected:	Mapk4, mitogen-activated protein kinase 4 ( MGI:2444559)
Sequence:	sense strand is shown >T50662 TGAGCCAGCAAGCCTCTTTCTGGAGATTGCACAGTGGGTCAAGAGCACACAAAGTGGCTCTGAGCGTGCC AGTCCTCCCCCTGATGCCCCAGAGCCCCGTCTCTCTGCCTCACCCCCGGGACACCCAACACCCATCGATG GGGGTGCCAGTCCCCAGTTCGACTTAGACGTGTTCATCTCGCGTGCCCTAAAGCTCTGCACCAAACCTGA GGACCTCCCGGAAAACAAACTGGGCGACCTGAATGGCGCGTGCATCTCTGAGCACCCTGGCGACCTCGTG CAGACAGAGGCCTTCTCCAAAGAAAGGTGGTGAGCACAGAAGGGGTGCGGGAAAACTCCCGGAGCAGGAT CCCCATCCAGAAAGCTGGACCACTTCCGTGCTCCCTCGCACTCCTTCATCCCCTCTCTGCTGCGTTGGGG TTAGCAGAACACACGCGGATCCGGGGAGAAAGAAGAATGTCCATTTCTTAAACTGCCTTAATAACTAGCC TTTAACCTCTGGGAGCGGGTTTGAACCGAGCCTGGCTGGGAGCCGTCAGTTTCCCATCGAAGGGAAGGTC TGTTGAGATTGTGTGCAAGGGAAGAAAGGGATCTAGGGCAGCAGTCTACGCCCTGCAAGAGTAGAGGGGT CAGAAACAGCTTGCAGACAGTTTCCAAGCCACGTGACCCACTTAGTCTCAAA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9856 same embryo
	EMAGE:9854 same embryo
	EMAGE:9855 same embryo
	EurExpress:euxassay_018744 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source