EMAGE mouse gene expression spatial database

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EMAGE:10022

Rabac1 Rab acceptor 1 (prenylated) ( MGI:1201692)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000239. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000239_01	euxassay_000239_02	euxassay_000239_03	euxassay_000239_04

euxassay_000239_05	euxassay_000239_06	euxassay_000239_07	euxassay_000239_08	euxassay_000239_09

euxassay_000239_10	euxassay_000239_11	euxassay_000239_12	euxassay_000239_13	euxassay_000239_14

euxassay_000239_15	euxassay_000239_16	euxassay_000239_17	euxassay_000239_18	euxassay_000239_19

euxassay_000239_20	euxassay_000239_21	euxassay_000239_22	euxassay_000239_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10022_wholemount_strong.wlz

10022_wholemount_moderate.wlz

10022_wholemount_weak.wlz

10022_wholemount_possible.wlz

10022_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10022_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata

weak

regional

weak expression: see section 13 14 15 16 17

metencephalon

moderate

regional

moderate expression: see section 09 10 weak expression: see section 11 12 13 14 15 16 17

facial vii ganglion

moderate

homogeneous

moderate expression: see section 04 05 06 20 21

glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 05

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

vagus x ganglion

moderate

homogeneous

moderate expression: see section 08 09 18 19 20

spinal cord

weak

regional

weak expression: see section 12 13 15 16 17

spinal component of peripheral nervous system

weak

homogeneous

weak expression: see section 14

dorsal root ganglion

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T272
Entity Detected:	Rabac1, Rab acceptor 1 (prenylated) ( MGI:1201692)
Sequence:	sense strand is shown >T272 GTCCCGGCTGGGGTGGGGCCGCCTAGGTGCTGCTGGGCTCCGGGCTATTTACAGCGGTTCTATCCACTGC AGTGCAGACATGGCGGCCCAGAAGGACCAGCAGAAGGATGCCGAAGGGGAAGGGCTGAGCGCCACGACCC TGCTGCCCAAGCTGATTCCATCCGGCGCAGGCCGAGAGTGGCTGGAGCGGCGCCNGGGCGAACCATNCCG GNCCCTGGGGGCCAC
Notes:	The probe template was PCR amplified from IMAGE:2655184 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2655184 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10024 same embryo
	EMAGE:10023 same embryo
	EMAGE:10021 same embryo
	EurExpress:euxassay_000239 same experiment
	MGI:4827587 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source