EMAGE mouse gene expression spatial database

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EMAGE:10271

Slc25a30 solute carrier family 25, member 30 ( MGI:1914804)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019708. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019708_01	euxassay_019708_02	euxassay_019708_03	euxassay_019708_04

euxassay_019708_05	euxassay_019708_06	euxassay_019708_07	euxassay_019708_08	euxassay_019708_09

euxassay_019708_10	euxassay_019708_11	euxassay_019708_12	euxassay_019708_13	euxassay_019708_14

euxassay_019708_15	euxassay_019708_16	euxassay_019708_17	euxassay_019708_18	euxassay_019708_19

euxassay_019708_20	euxassay_019708_21	euxassay_019708_22	euxassay_019708_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10271_wholemount_strong.wlz

10271_wholemount_moderate.wlz

10271_wholemount_weak.wlz

10271_wholemount_possible.wlz

10271_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10271_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

moderate

regional

moderate expression: see section 09 10 17 18 weak expression: see section 19

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 14 15

facial vii ganglion

weak

regional

weak expression: see section 03 04 05 19 20

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 17 18

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21

cervico-thoracic ganglion

weak

regional

weak expression: see section 10 16

cervical ganglion

weak

regional

weak expression: see section 16

thoracic ganglion

weak

regional

weak expression: see section 13

dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 12 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T56029
Entity Detected:	Slc25a30, solute carrier family 25, member 30 ( MGI:1914804)
Sequence:	sense strand is shown >T56029 GAAGGGTGTGTCCCTCACAGCCCAGAGGGCAGCCATTGTGGTCGGCGTGGAGCTTCCAGTCTATGACATC ACCAAGAAGCACCTGATACTCTCAGGCCTGATGGGAGACACTGTCGCAACGCATTTTCTCTCGAGCTTCA CCTGTGGCCTGGTGGGGGCCTTGGCCTCAAACCCAGTTGATGTTGTGAGAACCCGCATGATGAATCAGAG AGCCCTTCGAGATGGCAGATGTGCGGGCTATAAGGGTACCTTGGATTGCCTATTGCAGACATGGAAGAAT GAAGGGTTTTTTGCTCTATATAAAGGATTTTGGCCAAATTGGTTGCGCCTTGGTCCTTGGAATATCATTT TCTTTTTGACTTACGAACAACTGAAGAAACTGGACTTGTGACCACAAGTCGTCGTTCTGACAAACCGACT TCCGCGACGGTAGCCCCTCCTGTGCTTCTGGCTGCCTCTTACTGCACAGCTCATCATGGCTTCTGGGATG GGAGCAATGGATGGAGACCAGGTTCCTCAGATTGCCATGCGTTGGTCCTGGATGCTTTCATCTGTGTTCA GAGCCTAGAGCATAGGAACACCCACTCACCGGTGGTTACCATCGAAGTGCCCTTGACATCCTAGATAACT GATGTACATAACAGATCGTCCTTGCTGTAGAAAACCTGCAAGGAAATGAGAACCGCCTGCTGCTTTCTTC CTGGAGAGTAGCCTCGCTGCCTCTGGTCTTCTGGCTGTGTACATCGCCCCTCTGAGGGGGCTCCTGAAGT GGGGGAGCACTGTTATCCCCCTACCATCGGGAGCTTTCGTTTCCTGTCAGTGAAGAGATTTTACATTGGG ACAGTTGTAGGAAGTACGTCTCTAGCATACTGCACGGCTTCATGGTTCCTGCTTGGGCAGAATCTTTCAC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GAAGGGTGTGTCCCTCACAG; Reverse Primer - name:unspecified, sequence:GTGAAAGATTCTGCCCAAGC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10269 same embryo
	EMAGE:10270 same embryo
	EurExpress:euxassay_019708 same experiment
	MGI:4828154 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source