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EMAGE:1057

Tcf4 transcription factor 4 ( MGI:98506)
TS14 (16 Somite no.)
in situ hybridisation

Data Images
EMAGE:1057 EMAGE:1057
Figure 4D of Ishibashi & McMahon, 2002 [PMID:12361972] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 4B of Ishibashi & McMahon, 2002 [PMID:12361972] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
D and B are both TS14 embryos treated with the same probe (B has 14 somites whereas D has 16 somites). Only the data from D has been used for spatial mapping in this EMAGE entry. Image annotations: Arrowheads indicate the diencephalon-midbrain boundary; Arrow indicates r5 expression.
Expression Pattern Description
Spatial Annotation:
EMAGE:1057Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1057_wholemount_strong_3D_1.wlz
1057_wholemount_moderate_3D_1.wlz
1057_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1057_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
future diencephalon
strong strong
regionalAt 14 somites (Fig 4B): Expression first detected in diencephalon; robust localized expression in alar plate of P1/2 region. At 16 somites (Fig 4D): Expression levels in diencephalon are increased.
rhombomere 05
detected detected
At 14 somites (Fig 4B).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2676489
Entity Detected:Tcf4, transcription factor 4 ( MGI:98506)
Notes:The Tcf4 probe used in this study by Ishibashi & McMahon, 2002 [PMID:12361972] is of undefined sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:16 Somite no.
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + undefined
General Information
Authors:Ishibashi & McMahon, 2002 [PMID:12361972] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:12361972] Ishibashi M, McMahon AP 2002 A sonic hedgehog-dependent signaling relay regulates growth of diencephalic and mesencephalic primordia in the early mouse embryo. Development (129):4807-19
Links:MGI:2676614 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI