EMAGE mouse gene expression spatial database

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EMAGE:10597

Serpinb11 serine (or cysteine) peptidase inhibitor, clade B (ovalbumin), member 11 ( MGI:1914207)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000950. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000950_01	euxassay_000950_02	euxassay_000950_03	euxassay_000950_04

euxassay_000950_05	euxassay_000950_06	euxassay_000950_07	euxassay_000950_08	euxassay_000950_09

euxassay_000950_10	euxassay_000950_11	euxassay_000950_12	euxassay_000950_13	euxassay_000950_14

euxassay_000950_15	euxassay_000950_16	euxassay_000950_17	euxassay_000950_18	euxassay_000950_19

euxassay_000950_20	euxassay_000950_21	euxassay_000950_22	euxassay_000950_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10597_wholemount_strong.wlz

10597_wholemount_moderate.wlz

10597_wholemount_weak.wlz

10597_wholemount_possible.wlz

10597_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10597_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity respiratory epithelium

strong

homogeneous

strong expression: see section 09 12 13 15 16 17 18 19 weak expression: see section 10

nasal septum

strong

homogeneous

strong expression: see section 11

vomeronasal organ epithelium

strong

homogeneous

strong expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3919
Entity Detected:	Serpinb11, serine (or cysteine) peptidase inhibitor, clade B (ovalbumin), member 11 ( MGI:1914207)
Sequence:	sense strand is shown >T3919 GAAAGCAACAAAGGCATTGTTTGGGATAAGAATGGATCCCATCACCACAGCGAGTACTGAATTTTGCCTT GATGTGTTCAAAGAGCTGAGCAGTAACAATGTGGGAGAAAACATCTTCTTTTCTCCGCTGACTACGTTTT ATGCTCTAAGCATGCTTCTCCTGGGTACCAGGGGAAAGAGTGCTGAGCAAATGGAAAAGGTGCTCCACTA TGACAGCTTTTCAGGAGTCTTAAAAGCAAAGACGAAAAATTCTTCTGAGTGCAGCCAAGTTGGAATGATG CATCCTGATTTCAGAGCCCTGATTTCCCACATTAACCAACAGAACTCCCTCAGCGTTGCTAACAGAATCT ACGGGACGAGGTCAATATCATTCCACAAGCAATATGTAAGATGTTGTGAGAAACTGTACCAAGCCAAGCT GCAAACTGTTGATTTTGAACTATCTACAGAAGAGACAAGGAAAAGTATTAACGCTTGGGTTAAAAATAAA ACTAATGGAAAAATCACAAATTTATTTGCAAAGGGTACAATTGACCCATCCTCGGTCATGGTCCTAGTGA GTGCCATATATTTCAAGGGACAATGGCAAAATAAATTCCAAAAAAGAGAGACAGTGAAAGCCCCCTTTCA CATGGGTGTGGGTAAAAGTACAGTTGTGAATATGATGTATCAGACTGGAACATTTAAGCTGGCTATCATA AAGGAGCCAGAGATGCAAGTCCTTGAGCTTCCCTATGCCAACAACAAACTAAGAATGATCATCCTGCTTC CTGTAGGCACAGCCAGCGTGAGCCAGATAGAAAAACACCTGAATGTGAAGATGCTTCGAGAGTGGACTAA CCCTTCTAACATGGTGGAAAGAGAAGTGGATGTTCACATCCCTAAATTCAGTCTTTCAGTAAAATACGAC CTAAACACCCTATTGAAATCCCTAGGCATGAGGGACATCTTCAACGTAGCCAATGCTGATCTCTCTGGAA TGTCACCGGACAAGGGCCTATATTTATCTAAGGTTGTTCACAAGTCATATGTGGATGTCAATGAAGAAGG CACAGAGGCAGCTGCAGCCACTGGGGAGAGTATTTCTGTGAAGCGACTCCCTGTCACAGTTCAGTTCACA GCCAATTGTCCTTTCCTGTTCTTTATCTGGGATGAGTCTGGCAATATTTTATTTGCTGGCAAGTTTGCTT CTCCATAGACAGAGGGCATCGTGGAGGCCTCAGAGATAGCTAAGAAGTACAGGGGCGAAGGGGCTGAACC TTTGCATCTCAACTTTTGGTCATCTGCAAAGCAGGTCTACTATCGCCTCAGACTTATCCTGTGAGTTAGT GAGCCCAAGACTCTAGCAGCTGTGTTAGAAGATCCATTTATACCAGTGCTCTTCTGGATTTAATATCTTT GAGACCTCATCTGAGTACAGAGTGGCATAATTGATTTAACAGAGAAGTTTATAAAAGGTTTGATATGTTT GGTTTTTCTATGAGTGAGATTGTGAATGAGTGATCTAGGGTTTCTACTTTGGCTGGATTTCTAAATATAA ATTTATTGCATGCCTATGGATTTCAGCTCAAACTTCTACATGGATCATAATATATAGACACAATGCTTTT AACTCTTTGAAGATAAGAATAAACTATATCCGTTAATAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2646766 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2646766 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10595 same embryo
	EMAGE:10598 same embryo
	EMAGE:10594 same embryo
	EMAGE:10593 same embryo
	EMAGE:10596 same embryo
	EurExpress:euxassay_000950 same experiment
	MGI:4827988 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source