EMAGE mouse gene expression spatial database

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EMAGE:10726

Abcg4 ATP-binding cassette, sub-family G (WHITE), member 4 ( MGI:1890594)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000997. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000997_01	euxassay_000997_02	euxassay_000997_03	euxassay_000997_04

euxassay_000997_05	euxassay_000997_06	euxassay_000997_07	euxassay_000997_08	euxassay_000997_09

euxassay_000997_10	euxassay_000997_11	euxassay_000997_12	euxassay_000997_13	euxassay_000997_14

euxassay_000997_15	euxassay_000997_16	euxassay_000997_17	euxassay_000997_18	euxassay_000997_19

euxassay_000997_20	euxassay_000997_21	euxassay_000997_22	euxassay_000997_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10726_wholemount_strong.wlz

10726_wholemount_moderate.wlz

10726_wholemount_weak.wlz

10726_wholemount_possible.wlz

10726_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10726_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

weak

regional

weak expression: see section 04 05 06 07 21 22 23

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 20

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vagus x ganglion

weak

regional

weak expression: see section 09 19

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 08 09 19 20

cervico-thoracic ganglion

weak

regional

weak expression: see section 11 12 18

cervical ganglion

weak

regional

weak expression: see section 10 18

thoracic ganglion

weak

regional

weak expression: see section 13 14 15 16 17

dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 12 13 18 19 20

liver lobe

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4318
Entity Detected:	Abcg4, ATP-binding cassette, sub-family G (WHITE), member 4 ( MGI:1890594)
Sequence:	sense strand is shown >T4318 GAGCCGGAGTGTCCACCTCCCGCGCCGCAACGCTGAGCATCCGGGTCGAACATCCCGGTTGAGCATCTCT GCTGAGCATCCTTGCGTCGCAGCGGCCGGAGACGCGTCAAGGCCGGCTGCGTGATGGCGGAGAAGGCATT GGAGGCCGTGGGCTGTGGACTGGGCCCTGGGGCTGTGGCCATGGCTGTGACACTGGAGGACGGGGCAGAG CCCCCTGTGCTGACCACGCACCTAAAGAAGGTGGAGAACCATATCACAGAAGCACAGCGCTTCTCTCACC TGCCCAAGCGCTCTGCTGTGGACATCGAGTTCGTGGAACTGTCCTATTCCGTGCGGGAGGGGCCCTGCTG GCGCAAACGGGGTTACAAGACCCTCCTCAAGTGCCTGTCTGGTAAGTTCTGTCGCCGAGAGCTGATTGGC ATCATGGGCCCCTCTGGGGCTGGCAAGTCCACATTCATGAACATCTTGGCAGGGTACAGGGAGTCGGGGA TGAAGGGGCAGATCCTGGTTAACGGGAGGCCGCGCGAGCTGAGGACCTTCCGCAAGATGTCCTGCTACAT CATGCAGGATGACATGCTACTGCCTCACCTCACAGTCCTGGAGGCCATGATGGTCTCTGCCAACCTGAAG CTGAGTGAGAAGCAAGAGGTGAAGAAGGAACTGGTGACAGAGATCCTGACAGCCCTGGGACTGATGTCCT GCTCTCACACGAGGACAGCTCTGCTCTCTGGAGGGCAGAGGAAGCGCCTGGCCATTGCTCTGGAACTGGT CAACAACCCGCCTGTCATGTTCTTTGATGAGCCTACCAGCGGTCTAGACAGCGCTTCTTGTTTCCAAGTG GTGTCCCTCATGAAGTCCCTGGCCCATGGGGGCCGCACCGTCATCTGCACCATCCACCAGCCCAGTGCCA AGCTCTTTGAGATGTTTGACAAGCTCTACATCCTGAGCCAGGGGCAATGCATCTTCAAGGGCGTGGTTAC CAACCTGATTCCCTATCTGAAGGGGCTTGGCTTGCACTGTCCCACCTACCACAACCCCGCTGACTTCATC ATTGAGGTGGCCTCTGGAGAGTATGGAGACCTGAACCCCATGCTGTTCAGGGCTGTGCAGAATGGCCTTT GCACCATGGCCGAGAAGAAGAGCAGCCCTGGGAAGAATGAGCTCCCTGCCCACTGCCCCACTTGCCCTCC GGAGCTGGATCCTATTGAAAGCCACACATTTGCCACCAGCACCCTAACCCAGTTCTGCATCCTCTTCAGG AGGACTTTCTTGTCCATCCTCAGGGACACGGTCCTGACCCACTTGCGGTTCATGTCACATGTGTTGATCG GCGTCCTCATTGGCCTCCTCTACCTGCACATTGGCGATGATGCCAGCAAAGTCTTCAACAACACCGGCTT CCTCTTCTTTTCCATGCTCTTCCTCATGTTTGCAGCCCTCATGCCGACCGTGCTCACCTTTCCCTTAGAG ATGGCGGTCTTCATGAGGGAGCACTTGAACTACTGGTATACTCTCAAAGCCTATTACTTGGCCAAGACCA TGGCTGATGTGCCCTTCCAGGTGGTATGCCCAGTGGTGTACTGCAGCATCGTGTACTGGATGACAGGCCA GCCAGCCGAGACCAGCCGCTTCCTGCTCTTCTCAGCCTTGGCCATAGCCACCGCCTTAGTTGCCCAGTCT TTGGGACTCTTGATTGGCGCTGCCTCTACCTCCTTGCAGGTGGCCACTTTTGTGGGCCCAGTGACTGCCA TCCCTGTCCTCTTGTTCTCCGGCTTCTTCGTCAGTTTCAAGACCATCCCCACTTACTTGCAATGGAGCTC CTACCTCTCCTATGTTAGGTATGGCTTTGAGGGTCTGATCCTGACCATCTATGGTATGGAGCGAGGACAC CTGACCTGCTTAGATGAACAGTGCCCATTCCGGGACCCACAAATCATTTTGCGTGAGCTGGATGTAGAGG AGGCCAAACTCTACATGGACTTCCTGGTCCTGGGCATTTTCTTCCTTGCCCTGCGGCTGCTGGCATACCT TGTGCTCCGGTACCGGGTCAAGTCTGAGAGATAGAGCCTGGCTCCTACCTCTTCTGGGCAGGAAGGCCCC AGTTCCAGCCCTTTGGGGCTGTTTTAAACTTGTACTTGGGCATTGGTTGCTGGTGCAGCTGCCCTCCTCT CCCTCGGCTGCTACTCAGGCTGGCTGGCTGTGGGCTGTGTCTCCCAGTCTCAGCCCCGGAGGTAGGGCTC CAGCCCTCCCGTCAAGGCCAGGAGTCTTCCCAAGTTGATGCAGTTGGTAGCTTCTTCCCTACTGTCTCCA ACACCTGCATGCAAAGACTGCTGGGAGGTTGCTGCCCTCCTCTGCGGAAAGCACCCTCCTCTGTTGTTTG CCTGGGAGCTCCACGTTCTCTAGGCCTTGACTTACAAGTGATCAGATTGACTCCCCTCTGGGGTCCCCGC CACACTCTGCTGTCAGGTTGGAGTCCCAGGGCTGGGCCCTGGTGGGGTCTCCAGGGAGTCCCATCATGGA CATTGAATGGAGCAAGGAGGGACTCTGGAAGCTTCCTCCTCTTTCTCTTTCCCTCCCAACACCCACTTTG GCTAATTTGGACTATCTGTTGGGACAGAAGCCGGGCTTTCTGTCTGGGCCACCACTCCCAATCCTGGAGA TTGGAGAGAGCATAGGATGTCCCGGCCCCTTCCCTAAGCCTTCGGTCTGGATAGACCTCAACATCACCTC TGCAATAATGTCTGTGTCACTCTCCCATCGGCCACAGGACCTGGAGAATGCACTTTATTCTGGGGGGTGA GGCGGGGAAGCCCCCATCCTCCTTCCTTGCTGCTCCTGGGGCTCAGTTCCACAGGCTCCTTTCCTCTCCA GAATCACATCACAGGCACACAAAGGCCGCCAGGCAATCTCAGCCCTACACAGGCACCATCCCTTGTGGCT CAGAGGAAGAGTGGCCGTGGCAGGGAGCAGTCCCAACGTCCTCCTGCAGAATCCCACTTTGGAGGCTAAG AATGAGACTGGTGTGACCCAGTATGACTCCAGGCTCGGCAGGTGTCTATCTCCCAGTCTTCCCTCCCTCT TGAGCCCGGGGTGGCGCTGGCCTTCTGGAGGTCAGAACCCAACAAGCACAAGAATGGGCATAGCTGGCCT CGGCCACTGCTCCCCCATGGCCCTCCTTTTGTGCCCCAAGCTGACACTCACTCACCTGCCCCTCCTCCAG CTGCCTGCTGCTCACTCAAACTTCTGTTCACATGTATTCATCTTCCGGTCAGCAGCTGGTTCCTGGACAT CAGAGCGGCGGGGCCTGGACATCAGGTGGCAGACACACCTGTCTATCTTCTTGGTGGGTCCAGGATCATC CTATATCTGGTTTCTCCTCCTCCAGGACTCTTTCCCCAGTCCCTGTGCTCACTCCTCTGTATCTGCCCCA GGTTCTTCTCATTACACAGAGACCAGTTGTATTTGTGGGATTTCACCCATTATTAATAAAACCTATATTT ATACAGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4511456 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4511456 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10727 same embryo
	EurExpress:euxassay_000997 same experiment
	MGI:4822873 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source