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EMAGE:10730

Ate1 arginyltransferase 1 ( MGI:1333870)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10730 EMAGE:10730 EMAGE:10730 EMAGE:10730 EMAGE:10730
"Pseudo-wholemount" of euxassay_002987. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002987_01 euxassay_002987_02 euxassay_002987_03 euxassay_002987_04
EMAGE:10730 EMAGE:10730 EMAGE:10730 EMAGE:10730 EMAGE:10730
euxassay_002987_05 euxassay_002987_06 euxassay_002987_07 euxassay_002987_08 euxassay_002987_09
EMAGE:10730 EMAGE:10730 EMAGE:10730 EMAGE:10730 EMAGE:10730
euxassay_002987_10 euxassay_002987_11 euxassay_002987_12 euxassay_002987_13 euxassay_002987_14
EMAGE:10730 EMAGE:10730 EMAGE:10730 EMAGE:10730 EMAGE:10730
euxassay_002987_15 euxassay_002987_16 euxassay_002987_17 euxassay_002987_18 euxassay_002987_19
EMAGE:10730 EMAGE:10730 EMAGE:10730 EMAGE:10730
euxassay_002987_20 euxassay_002987_21 euxassay_002987_22 euxassay_002987_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10730Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10730_wholemount_strong.wlz
10730_wholemount_moderate.wlz
10730_wholemount_weak.wlz
10730_wholemount_possible.wlz
10730_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10730_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 21 22
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 08 19 20
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 09 18
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 07 08 09 18 19 20
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4242
Entity Detected:Ate1, arginyltransferase 1 ( MGI:1333870)
Sequence:sense strand is shown

>T4242
GTGGGGTGGTGGAGGCGAAGGCGGCGGTAGCGGCGGCTGCGTAGGACTGGGTGGCGCAGCGGCGGCGGGG
TCGCGGACATGGCTTCTTGGAGCGCGCCTTCACCTAGCCTTGTAGAGTATTTTGAGGGCCAGACCTCCTT
CCAGTGTGGCTACTGCAAGAACAAGTTGGGCAGTCGCTCCTATGGCATGTGGGCACATTCCATGACAGTG
CAGGATTATCAGGATCTTATAGACCGAGGATGGAGAAGAAGTGGGAAATATGTGTACAAACCTGTCATGG
ATCAAACATGCTGTCCTCAGTATACAATAAGGTGTCATCCTTTACAGTTTCAGCCATCAAAATCTCACAA
GAAAGTTTTGAAAAAAATGCTGAAATTTCTGGCTAAAGGAGAGATCTCGAAAGGCAATTGTGAGGATGAG
CCCATGGATTCTACAGTGGAGGATGCTGTTGACGGTGACTTTGCATTAATTAACAAGCTGGATATAAAGT
GTGATCTCAAAACACTCAGTGACCTCAAAGGAAGCATAGAGAGTGAAGAGAAGGAGAAAGAAAAGAGTAT
AAAGAAAGAAGGGTCTAAAGAATTCATTCATCCACAATCTATAGAGGAGAAGTTGGGCTCTGGTGAACCA
TCACATCCAATCAAAGTTCATATTGGTCCTAAGCCAGGCAAAGGGGCTGACTTGAGTAAGCCTCCATGTC
GGAAAGCAAGGGAAATGAGGAAAGAAAGGCAAAGATTAAAACGGATGCAGCAGGCCTCAGCTGCAGCCTC
GGAGGCTCAAGGTCAGCCAGTCTGTTTGTTACCAAAGGCTAAATCCAACCAGCCCAAGTCACTGGAAGAT
TTGATTTTTCAATCTTTACCAGAAAATGCATCGCACAAGTTAGAGGTGAGGGTGGTAAGATCTTCTCCAC
CAAGTCCTCAGTTCAGAGCCACATTTCAGGAGTCTTACCAGGTCTATAAACGCTACCAGATGGTTGTTCA
CAAGGACCCGCCTGATAAGCCAACCGTGAGCCAGTTTACAAGATTCCTTTGCAGCTCACCATTGGAGGCA
GAGCACCCTGCTGATGGACCAGAATGTGGTTATGGCTCCTTTCACCAGCAGTACTGGCTCGATGGGAAGA
TCATTGCTGTGGGGGTGTTAGACATTCTCCCGTACTGTGTGTCTTCTGTGTATCTCTACTACGATCCTGA
TTATTCATTTCTGTCTTTGGGTGTCTATTCAGCATTAAGAGAAATTGCTTTTACTAGACAACTGCATGAG
AAAACATCGCAACTCAGCTATTATTATATGGGTTTCTACATTCATTCCTGTCCCAAGATGAGATACAAGG
GTCAATATAGACCTTCTGATTTGCTGTGTCCTGAGACGTATGTCTGGGTGCCCATTGAGCAGTGCCTGCC
TTCTCTGGACAACTCCAAGTACTGCCGTTTCAACCAGGACCCCGAAGCAGAGGATGAAGGACGCAGTAAG
GAACTTGACCGACTAAGGGTGTTTCACAGACGATCTGCCATGCCTTACGGTGTTTATAAGAATCACCAAG
AAGACCCAAGTGAGGAGGCTGGTGTGCTGGAGTATGCAAACCTCGTAGGACAGAAGTGCTCGGAGAGGAT
GCTGCTGTTCAGACACTGAAGGCCTGCACCTCAGCCCAGCGCCAGCCTGGCATCTCTTGCCACAGTCCCA
CCTGCTTCTCGGCTCCTGCAGCCATTCACCAAGTACATTCGTAGAGCTTTTTAAAAATATTTTGTGGAAT
TGTATTTATGAGAATCTTGTGGTTAATATAAAGATTTTAAAATATTCTATGACCTAGCCCTTTAATTGGG
ACTTGTCCTTTGCAGCTTGCTTCTGGAGCAAGGTGTGCTTCATGTCTCCAGTGAATATTTAGGGAAGGAA
AATGAAGAAGTCAATATAAGTCATTTTGTCACAGTTACTGTTCTGTGCTGGAGAAATTCTGACACAGAGA
CTGTTCTTTTAGACCAATGGTTCTTAACCTGTGGGCTCACCACACTCACAGGCCGAATGTTAGATAGCCT
GTGTGAGATCAACTCATAATAACTCATAATCGTAGCAAAATTACAGTTATGAAGTAGCAACAAAATAATT
TTATGGTTTGGGGTGGGATCACCACAACGTGAGGAAATGTATTAAAGGATGGCAGTATTAGGAAGGTAGA
GAAAGAACCACTGGCTAAGACCCATGCAAGACATTAGCTTATGCTGGGAAATTCAGATGGGCTTAAAGGT
GAAGAGCACATTCCATCCCAGGAAATGTGCCTTTCCCACAAACCAGAACCCTGTTACTCTGCTTGATAGT
AAACCTGCCAGTGTTGTAGTGTATAATAGACAACAGGAACTGTAAACATTGCAAACATCAGGTGTTCACT
TTGGGTGTCTGTCCAGGACTGTCCTGCCTATGTCTGTTGTCTTGGCATAATTGTTTTCCTTGTCACTAGT
GTTCCCTTGTGCCCCAGAGTTATCTCTGTTTGGATGATAAAATATGCAGTGATCTTAGTGGTGTTTGTTC
TATTTCTACAAATATAAGAAACAACTTTCTAAAGAAACGTCGTAACTCCTGCTTGATCTGAGGCTTATCC
AAAGAAAATAGCTTCCTACTTTCCATTCTCTGGAACTCAGACGCTTTTTTGTGGTTAGTGATAGAAGTGA
TTATTATCTAGTCCCGTAGCTATGCACAGCCGTAGTGGAGTTGGTGGGGCCTCTAGCCTCTGCATTCCTC
TTCTAGTTGTGGGTCAGAGAGTAGGCGCTGCCATGGTACTTCAGAATGGAGACCAGCCATTCACTTTCAT
TCTTGTGCTGTTGGGCCTTCTGCTTGTTCACCAAATGCCTGCTGTCAAGGAGGCAGGCAGCCAGCTACTT
CGTGAGGCACATCAGACCTGGTGAGGAGAGGCTTCCCTCAGGTGCTCGGGAAGCTGGATGCACAGGTTAG
CCCTTTGTTTGAACTTGTTCTCAGCTAGCCCCTTCTAGAAGTGCTTCCAAGCCTGGCCTTGGAGCTGCTG
AGAGCACCTATCCCACAGTGAGGGTAGGGTTGTTACTTTATTTCTTTTCATAAAAATCACTCCAATGGGA
AGCACCTGGAACGCTAAGAAATTAAAAGTCGTTTGAAATCCTCCCAGGCATAGAGAAAGGAGCATTATGG
AAACCCACAAATCTCTTGTCCACCCCAAATAAAACTGTGCATTCCTTGCTATGTAGTTGCCCTCGGTGAA
TGTGTGTGAGATTGAAGATTGCAGAAGATGTGATAGGTAATCTCAGTATTCTTCAGTAGGATGGGATCAC
ATATATGACCTTGGCATTGGGCAGCCAGGCATATCCTGGTAGGTGGTATAGCCATGCTACCAGAGGGGTT
TGTTTTTGGGAGGGGGCGGGGGGTCAGCCCAACATCTTACCCTAGCTCTGAGTTTCTCCTGCAGAGAATA
GAGGAAAATGGCTCACTGTCCCTGTTTCTAGACTGATGCGCCTAACCTAGGGTGGCCCTACTGAGGCTTC
AGAGCGTCTTGGGCATGAAAACTCTCTCTTCAGCTCAGTTGCTTTGTATTCATTCTTTCCTCCCTCCTCC
CTCTATCCTCTCCTCAATACAGTTTTTTTCGACTAGTATGAAACCTTAGGGTTCTAGATGTTGTCGAAGA
TGTGTCCTTGGGTGGGACTGAGGAGATAGCCCAGTTAGTACAGTGCCTGAGTTCAGTTCTCTAGAGCCCA
TGTAAAATGTTGGGTCTGGTAGTGTGTGTTTGAAATGCAGGCACTGGGGATTCAGTAGAGGAGTACCTCT
TGGGCCCACTGGCCACTGGCCTAGCCACTGGGCATCGAGCCACTGAGAGACCCTGTCTAAATGGAGTTGG
ACAGCATTTATGAGAACATTAGGGGCTTGCTAATATTTTGAGCTCTTGAGTGAGTGTCCATCACAGCTCG
GTTAGGACAGTCAAGAAAGTATCCCAACTTGGCATTTTTGTAAAGTCTCGCTGAGGCGGCTGATTCTGTC
AGTGATTTTCATGTAAATAAAGATGCGTGCAAGCCTTTCAGGGACAGTGTCTGACTTCTCCAGGTAGGAA
GGTCTGTGTGTTCCATCTCCTTCTTAGTGTCCCCTTCTGAGTTCAGGTAGAGTCACACCTTCCTCCATGC
CTACAGCGTAGGGCTCATTTTGATTCTCAGTGTACCATCTTTATTTAAATTATTTTCAGTTCTCTGAGAC
TTTTGTTACTTTTATTACAGGTAATCTGAGCACTTTTATATTCTAAGATAGAAACTATATTATCAGATGA
ATATAGTCACTCAATTAAAATTATTTAGATAAAATTATTGTTTATGTTTTCTTTCAAGCAATCTATCCTA
TTTTTATCCATTTGCAAATGCCAGTTTTGTGTGGTTTCTTAGCATATCAAAATTATTATGTAGAAGAATG
TTTCTAGCTTTCTGAACAGCAAATAGTTTGGTGTGTAAATATTGTATTATTTCAGTGTGAATAAAGGTGA
GAGAGAATATGAACATTTAACCAAAGTGATTCTACTGTGGCTGACTTAAAAGAACGGAAGGAAGTGAGTG
GGGATTTGACACGTTTGAATTACGAGTTATTCAGTGTGCGTTCCTCTCCTACGGGAACCACTGGCCAGAA
GGCAAAGTAAGTCTGACTGCATTGGAGATTTTCGTTCTTGTAAAAAAGCCCAAAATAAAAATACTAACAA
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4503326 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503326 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10732 same embryo
 EMAGE:10731 same embryo
 EMAGE:10729 same embryo
 EMAGE:10728 same embryo
 EurExpress:euxassay_002987 same experiment
 MGI:4823289 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS