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EMAGE:10769

Wdr31 WD repeat domain 31 ( MGI:1918604)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10769 EMAGE:10769 EMAGE:10769 EMAGE:10769 EMAGE:10769
"Pseudo-wholemount" of euxassay_001283. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001283_01 euxassay_001283_02 euxassay_001283_03 euxassay_001283_04
EMAGE:10769 EMAGE:10769 EMAGE:10769 EMAGE:10769 EMAGE:10769
euxassay_001283_05 euxassay_001283_06 euxassay_001283_07 euxassay_001283_08 euxassay_001283_09
EMAGE:10769 EMAGE:10769 EMAGE:10769 EMAGE:10769 EMAGE:10769
euxassay_001283_10 euxassay_001283_11 euxassay_001283_12 euxassay_001283_13 euxassay_001283_14
EMAGE:10769 EMAGE:10769 EMAGE:10769 EMAGE:10769 EMAGE:10769
euxassay_001283_15 euxassay_001283_16 euxassay_001283_17 euxassay_001283_18 euxassay_001283_19
EMAGE:10769 EMAGE:10769 EMAGE:10769 EMAGE:10769
euxassay_001283_20 euxassay_001283_21 euxassay_001283_22 euxassay_001283_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10769Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10769_wholemount_strong.wlz
10769_wholemount_moderate.wlz
10769_wholemount_weak.wlz
10769_wholemount_possible.wlz
10769_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10769_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
homogeneousweak expression: see section 11 12 13 14 15
submandibular gland primordium
weak weak
regionalweak expression: see section 08 09 10 17
foregut-midgut junction
weak weak
regionalweak expression: see section 11 12 13 14 15 16
pancreas
weak weak
regionalweak expression: see section 09 10 11 16
lateral ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 12 13 15 16 17 18
telencephalon ventricular layer
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21
4th ventricle
moderate moderate
regionalmoderate expression: see section 17 18 weak expression: see section 05 06 07 08 09 10 11 13 14 15 16 19 20
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 08 09 10 11 12 14 15 16 17
anal canal
weak weak
regionalweak expression: see section 13
stomach fundus mucosa
weak weak
regionalweak expression: see section 02 03 04 05 06 07 08 09 10
hindgut
weak weak
regionalweak expression: see section 12 13 14
midgut
weak weak
regionalweak expression: see section 09 10 11 12 13 14 15 16
not examined not examined
regionalnot examined expression: see section 18
renal cortex
weak weak
regionalweak expression: see section 07 08 09 10 16 17 18
testis
weak weak
regionalweak expression: see section 05 06 07 08 17 18 19
urethra of male
weak weak
regionalweak expression: see section 12 13 14
left lung
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11
right lung
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5378
Entity Detected:Wdr31, WD repeat domain 31 ( MGI:1918604)
Sequence:sense strand is shown

>T5378
CCACGCGTCCGGTGGAAGCTAAGGATGCCTGCTACCCGTGCTTTCGGGCCTCGGTGCTAGCCCACCGAGA
TTAGGAAGTGGCTGTCTGACTTGTTTCCGAAGGCTGAATGAACTACAGATCATCAAATACAGCAGCTCAC
CAAGAAGATCTGGTTGCTTTGGGGGGAACACCCCCTCAGCGCCTGTGAGGGATGTTGTTACTTGACGGCC
AACTGAAACCAGCTCTTCCTCAGAAGGTCTTCTGTAGACTCTGGGCTGTGATGGGAAAACTGCAGAGCAA
ACTCAGACACAGCACTTACTTATACAGCAGGCCTGATGGAATTATAGAAGAGAGAGTCCAGACTAAAGCT
CCCCAAGAATACATCCCTGTTCACGTGGATACCGTCTCTGTGATTGCCACTCTGAACTCAGACCTTTGTA
TCTCTGGAGGAAAAGACAAGACCGCCGTGGCCTACAATTGGAAAACTGGGCGTATGGTGAAAAGGTTCAC
AGGGCACGAGCGGGAAATCACGAAGATAGCCTGTATTCCCAAAGCAAACCAGTTCTTCAGCGCCTCTCGA
GACAAGACAGTCCTGATGTGGGATCTGCAGGGCTCCTCGCACCCTAGGCAGCAGCTGTCTGGCCATGCCA
TGGTGGTCACTGGCTTGGCTGTGAGCCCAGACTCGTCCCAGCTGTGCACTGGCTCTCGGGACAATAGCCT
GCTTCTGTGGGATGTGGGGACCGGACAGTGTGTGGAGAGAGCGTCTGTCTCCAGGAACCTGGTCACACAC
CTGTGCTGGGTCCCGAGTGAGCCATACATAGTCCAGACCTCTGAGGATAAGACCATCAGATTATGGGACA
GTCGGGGGCTTCAGATGGCTCACATGTTTCCCACAAAACAGCATATCCAGACCCATTGTGAAGTCAGCGT
GGATGGACACACGTGTATTTCCTGCAGCAGTGGCTTTGGGGGTGAAGGCTGTGAAGCCACGTTGTGGGAC
CTAAGACAGACTAGAGACAGAATGTGTGAGTATAAGGGGCATTTCCAGACAGTTGCATCCTGTGTCTTTC
TACCGAAGTCCACGGCCTTGATGCCTATGATTGCTACCTCTGCACATGACTCCAAGGTGAAGATTTGGAA
CCAGGATACTGGAGCTTGCCTCTCCACCTTGTCTCTGGATGGATCAGGACCCCTGACCTCACTGGCTGTT
GGTGACATCTCCTCCTTGTTATGCGCAAGTTTCTACAGAGGAGTTCACTTACTCAGAGTGGACCACAGCC
AAGGACTGGAACTGCAGGAAGTGGCAGCCTTCTAAAATCAAGAGATATTGATTGGATAATATCAAACCAT
GGTCCCTCAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5364307 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5364307 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10766 same embryo
 EMAGE:10767 same embryo
 EMAGE:10765 same embryo
 EMAGE:10768 same embryo
 EurExpress:euxassay_001283 same experiment
 MGI:4829196 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS