EMAGE mouse gene expression spatial database

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EMAGE:10963

Pard6g par-6 partitioning defective 6 homolog gamma (C. elegans) ( MGI:2135606)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003448. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003448_01	euxassay_003448_02	euxassay_003448_03	euxassay_003448_04

euxassay_003448_05	euxassay_003448_06	euxassay_003448_07	euxassay_003448_08	euxassay_003448_09

euxassay_003448_10	euxassay_003448_11	euxassay_003448_12	euxassay_003448_13	euxassay_003448_14

euxassay_003448_15	euxassay_003448_16	euxassay_003448_17	euxassay_003448_18	euxassay_003448_19

euxassay_003448_20	euxassay_003448_21	euxassay_003448_22	euxassay_003448_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10963_wholemount_strong.wlz

10963_wholemount_moderate.wlz

10963_wholemount_weak.wlz

10963_wholemount_possible.wlz

10963_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10963_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 moderate expression: see section 20 21 22 23

thymus primordium

strong

homogeneous

strong expression: see section 13 14 15 16 17

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 11 18 19 20

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 20 21 22 23 moderate expression: see section 12 13 14 15 16 17 18 19

vibrissa

strong

regional

strong expression: see section 05 06 07 08 09 10 20 21 22 23

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 05 06 22 23

esophagus

moderate

regional

moderate expression: see section 14 15

stomach

moderate

regional

moderate expression: see section 04 05 06 07 08

meckel's cartilage

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 18 19 20 21 22 23

lower jaw incisor

weak

regional

weak expression: see section 13 14 17

lower jaw molar

strong

regional

strong expression: see section 06 07 08 09 10 19 20

upper jaw incisor

weak

regional

weak expression: see section 13 14 17

upper jaw molar

strong

regional

strong expression: see section 06 07 08 09 10 19 20

cranium

strong

regional

strong expression: see section 02

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 21 22 23

clavicle

moderate

regional

moderate expression: see section 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5945
Entity Detected:	Pard6g, par-6 partitioning defective 6 homolog gamma (C. elegans) ( MGI:2135606)
Sequence:	sense strand is shown >T5945 CCACGCGTCCGGGGCCCGGCGCCGGGCCCGGGCAGACGTTGGGGGGCGTCTCCGCGGAGACTCTCTCTGA GGCCCCTGAGCGGCGAGGGCGAAGGCGAGGCTGAGTGGGGCCGAAATCATGAACCGGAGTTTTCATAAGT CTCAGACCCTACGCTTCTACGACTGCAGCGCTGTGGAAGTCAAGAGCAAGTTTGGCGCCGAGTTCCGAAG GTTCTCCTTGGACCGCCACAAGCCTGGGAAGTTTGAAGATTTCTACCAGCTGGTGGTGCACACCCACCAC ATCTCTAACACCGAGGTGACCATCGGCTATGCTGATGTGCACGGGGACCTGCTGCCCATCAACAATGACG ACAACTTCTGCAAGGCAGTCTCAAGTGCAAACCCCTTGCTTCGAGTCTTCATCCAGAAACGAGAAGAAGC AGACCATTACAGCTTCGGAGCAGGCACTCTGTCACGGAAGAAAAAGGTGCTGGTGACCCTGAGGGATGAC GGTCTGCGTCGGCGTCCCCACCTCAACATCAGCATGCCGCATGACTTCCGCCCAGTGTCATCCATCATTG ACGTGGACATCCTCCCGGAGACGCACCGCCGAGTGAGGCTCTACAGACACGGTTGTGAGAAGCCACTGGG CTTCTACATTCGAGATGGCACCAGCGTGCGGGTGACGCCCCATGGGCTAGAAAAGGTACCAGGTATCTTC ATTTCTCGAATGGTACCCGGGGGCCTTGCGGAGAGCACTGGGCTACTGGCTGTGAATGATGAAGTCCTGG AGGTGAATGGGATTGAGGTAGCTGGAAAGACCTTGGATCAAGTCACAGACATGATGATAGCCAACAGCCA CAACCTCATTGTCACTGTCAAGCCGGCCAACCAAAGGAACAATGTGGTCCGCAGCAGCCGCACGTCCGGC AGCTCTGTGCATTCCACAGACAGCACCACCAGTCACCACAGCCTGCCAGGTGCCCATGTCCTGCAGAATT CTGAGGACGTGGAGAGTGACGAGGAAGCCGACATTGTCATTGAAGGGGCTCTAGAACCTCAACACATTCC CAAGACGCAGGCTGTACCTCCAGGCAGCCTCTCCAGGGCCAATGGCACCAGCCTTGCTCACGGGCTGCAC AGAAGGGACATGAGCCTGCATAGCTCTGGGAGGGAGAGCAACGGCAGCATCCACAGATTTCTCAGCTCTC TGAAGCCAGACCCCCGACACAGCCTGGTCCTCCCCCAAGGAGGGGTGGAGGAACATGGGCCAGCCATTAC ACTCTAGGGCTCGAGCCACTTGGGCAGGCTAGAGTCCCAGACAGACTGTGTGTTACCAAGACCCATGAGT GGCAAATATTAATAGCATGTAGTACTGTTAGATATATAGTGACTCCAAAGAGAAGCGCTTTCCATTTTTA TGTCAATTTTAAAATAAGAAAAATAATTGTGTACTTCATCTTTTCTTTTTCATCATCATTCCTACAACCT TATTCTTTATTTAAACTTTTAAACAAGAATACTATTTTTATAGGGTTTTTCCACAAACTTAAAATATGCA CAACTGCAAATTATCTGGGCCAGGCCTCAGTGCCTGTCCAGAACCTTTGTGGAGTGAAACACAACACAAA AGTGCAGAGAGTATTTTTAACATTTTTTTAAATACTTGTTTTTAAAATGAAGCTTTATAGATAACTTTTT AAAAACAGTAAAATTCATGTTTTTAACCAGTTCATGTAAAACTATCTTGTGATGCCCGAGTGTTCCACTG ACCCCGTGTGGCTGTGACAGAGCAATGGCTGGGCTCCATCACAGAGAGTCTGTTTCCACACTGTTTACAA CCAGCTCAGAAAGCCCTCTGAACTATACTCAAGACCCGCTGGGATTACAACGCCCTGCATCTGCGGAGCT GCCCCAGCCGTGACAGTCAGGCGGTCACTGCTGTTGGCGGCTCCAGTGGATTTAGGAGCCCAGCAACTCT GCTGGCTAGAAGGTGAGGCCAGTGGTAGCACGTGGGATTAAAAGCCAACTGTTCCATCCGTGTTTCCTCT GGGTTGGCTGACTAGGGCGGTTTTAAGGCATCTTCAGTAGAAATGAGTCTCGTACCTCTGAAGGCCCGCA GCCGTGGGTGAATGTGCATGTTAGCTAGTTATTTTTATAAGAGGAGATTCGAGCATGTCAGTGCCTGTTC CTAGAATAGCGATCCGTGGCAGGAATCATCTTAACTACAAGTCACCGTGGAACTTAAATAGTTTTGACAG ATCACTTTTCTTTCTCCTTAGAGGTGGTGTATCAATGTCTGACCCAGTTAAATTTTATATGAATTGCGCC TTCTGTTTGGATACAGTGTTGGGAGAGAATATCCAATTGATTAACTGAGGACAAACTGACCAAGTGACTC CTTCATGTAAATAAAGGTTTTAAAGCATCTTCGTCTTCCAGGTCGAGTGCCCCAGCTGCTGCCCTTATGG TCTAGTGCCACCTGCTGGTAAGACGCAGAACTGTCCTGACTTCATGAATGCCAGATGGAGAATGCATCTC AGCATTGGCCTTTGTCCAGAACTCATACTGTCAAATAGTGTTGTTCCTTCCTTTGGGGAACTGTTTCCTG GGCAGAGGTGGCCTTGGTTAAGGATCGTTTCCTTGCCCTGTGCCTGTTTCTCCGTTATGTGCGGAGTGTC CACACAAGGAGAGCAAGATACTCCCCGGGCCTCGGTGAGGATCCAGGCACCCAGACTCATGGCTTCTAAA CTGCTAAAAGCAACTGTTTTGTTTCCATGTTGCTGCAGTGATCTGGAATGTGCTTTACTAAGACAAATAT TACAGAACCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3483559 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3483559 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10966 same embryo
	EMAGE:10965 same embryo
	EMAGE:10964 same embryo
	EMAGE:10968 same embryo
	EMAGE:10967 same embryo
	EurExpress:euxassay_003448 same experiment
	MGI:4827051 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source