EMAGE mouse gene expression spatial database

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EMAGE:11030

Abtb1 ankyrin repeat and BTB (POZ) domain containing 1 ( MGI:1933148)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003399. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003399_01	euxassay_003399_02	euxassay_003399_03	euxassay_003399_04

euxassay_003399_05	euxassay_003399_06	euxassay_003399_07	euxassay_003399_08	euxassay_003399_09

euxassay_003399_10	euxassay_003399_11	euxassay_003399_12	euxassay_003399_13	euxassay_003399_14

euxassay_003399_15	euxassay_003399_16	euxassay_003399_17	euxassay_003399_18	euxassay_003399_19

euxassay_003399_20	euxassay_003399_21	euxassay_003399_22	euxassay_003399_23	euxassay_003399_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11030_wholemount_strong.wlz

11030_wholemount_moderate.wlz

11030_wholemount_weak.wlz

11030_wholemount_possible.wlz

11030_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11030_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 23 24 moderate expression: see section 05 06 07 18 19 20 21 22 weak expression: see section 04

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 07 08 14 15 16

pons mantle layer

moderate

regional

moderate expression: see section 05 16

facial vii ganglion

strong

regional

strong expression: see section 03 04 moderate expression: see section 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 17 moderate expression: see section 18

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 16 17 moderate expression: see section 01 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 07 08 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 17 moderate expression: see section 18

ventral grey horn

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 15

cervical ganglion

strong

regional

strong expression: see section 08 16

thoracic ganglion

strong

regional

strong expression: see section 12

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 15 16 17

otic capsule

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 15 16 17 20 21 22 23 24

cornea stroma

moderate

regional

moderate expression: see section 01 02 22 23 24

viscerocranium

moderate

regional

Expression in the turbinate bone.

vomeronasal organ

moderate

regional

moderate expression: see section 05 06 07 08 18 19 20

meckel's cartilage

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 15 17 20 21 22 weak expression: see section 18 19

nucleus pulposus

moderate

regional

moderate expression: see section 12 13

basisphenoid bone

strong

regional

strong expression: see section 23 24

exoccipital bone

strong

regional

strong expression: see section 03 moderate expression: see section 04 20

temporal bone

strong

regional

strong expression: see section 03 moderate expression: see section 04 20

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 21 22 23 24 moderate expression: see section 05 06 18 19 20

clavicle

moderate

regional

moderate expression: see section 08 09 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5948
Entity Detected:	Abtb1, ankyrin repeat and BTB (POZ) domain containing 1 ( MGI:1933148)
Sequence:	sense strand is shown >T5948 CGGACGCGTGGGCGACTCCCGAGATCACTCGGCCGGGGGTCCAGTCTCTTTCGCCATGGATACCAGCGAC TTGTTTGCCAGCTGCAGGAAAGGGGACGTGGGCCGTGTGCGGTACCTGCTGGAGCAGCGAGACGTGGAGG TGAACGTGCGGGATAAGTGGGACAGCACCCCTTTGTACTATGCCTGCCTCTGTGGGCATGAGGAGTTGGT GCGCTACCTGTTGGCTAATGGAGCCCGCTGTGAGGCCAACACCTTTGATGGAGAGCGGTGTCTCTACGGA GCGCTAAGCGACCCTATTCGCCGTGCACTGAGAGACTACAAACAGGTCACTGCGTCCTGCAGGAGGCGGG ATTACTATGACGATTTCCTGCAGCGGCTTCTGGAACAGGGCATCCACAGCGATGTGGTCTTTGTGGTACA CGGAAAGCCGTTCCGGGCCCATCGCTGTATCCTCGGTGCTCGCAGTACCTACTTCGCCAACATGCTGGAC ACCAAGTGGAAGGGCAAGAGCGTCGTGGTTCTCAGGCACCCCCTGATCAACCCGGTGGCCTTTGGGGCTC TACTGCAGTACCTGTATACAGGCCGCTTGGACATCGGTGTAGAACACGTGAGTGACTGTGAGCGCCTGGC CAAGCAGTGCCAGCTGTGGGACCTGCTGGATGACCTGGAGGCTAAGTGCGAGAAGGTGTCTGAATTTGTG GCTTCCAAGCCTGGCACGTGTGTGAAGGTGCTGACTATTGAACCTCCTCCAGCAGATCCCCGGTTACGGG CAGATATGGCCCTGCTAGCTGACTGTGCCCTGCCATCTGAGCTGCGGGGTGACCTTGGAGAGCTGCCCTT CCCATGTCCCGATGGCTTCAGCAGCTGCCCCGATATCTGCTTCCGAGTGGCTGACTCCAGCTTCCTCTGC CACAAGGCTTTCTTCTGTGGCCGCAGTGACTACTTCCGGGCCCTTCTGGATGACCACTTTCAAGAGAGTG AGGAGCCTGCAGCCTCTGGAGATCCCCCAGTTGTCACCCTGCATGACATCTCCCCGGACATCTTCATTCA CGTGTTGTACTACGTATATAGTGACCATACCGAGCTGCCTCCTGAGTTGGCCTACGATGTTCTGAGTGTG GCTGACATGTACCTTCTGCCTGGCCTGAAGCGGCTGTGTGGCCGCAGCCTGGCCCAGCTGCTGGAGGAGG ACAGTGTGGTGGGTGTTTGGCGCATAGCCAAGATGTTCCGGCTGGCCCGGCTGGAGGACCAGTGTACCGA GTATATGGCCAAAGTCATAGAGAAGCTGGTAGAGCGGGAGGATTTCGTGGAGGCCGTGCGGGAAGAGGCG GCAGCTGTAGCGGCCCGGCAGGAGACAGACTCCATCCCGCTCGTCGATGACATCCGCTTCCATGTGGCCA GCACGGTCCAGACATACAGTGCCATTGAAGAGGCACAGCAGCGACTGCGGGCACTCGAGGACCTGCTCGT GTCCATTGGCTTGGACTGCTAAGCCCTTCCGTGGCCAGGGCAGCCACGGTGACAGGCATGCGCAGTGTGT GCACGCAGCAAGATGGCATTACAATGGGCAGAGGGGACCTCACAGGATGTCCTTGGGATGAGCCTCCTCC TCCAGTGCACAAGGAAGCCCCAAGATTTGGGGCAGGCACCACTCAGGGAAAACTGGGGTACGGTAGGCTT TGAACTTAGTCCCATCTCCCCCAGCGTCCTACCTGCTCCCTCCTGGTTCTCACCCAGCCCTGGCTTGCTT CGGCTATTTCCGCATACAGGGCTGGACAGGACGTAGGGGGTGTGTGGCAATAAAGCTTGAATTCACTCTG GAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3483893 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3483893 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11032 same embryo
	EMAGE:11034 same embryo
	EMAGE:11033 same embryo
	EMAGE:11029 same embryo
	EMAGE:11031 same embryo
	EurExpress:euxassay_003399 same experiment
	MGI:4822891 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source