EMAGE mouse gene expression spatial database

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EMAGE:11053

Stc1 stanniocalcin 1 ( MGI:109131)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001173. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001173_01	euxassay_001173_02	euxassay_001173_03	euxassay_001173_04

euxassay_001173_05	euxassay_001173_06	euxassay_001173_07	euxassay_001173_08	euxassay_001173_09

euxassay_001173_10	euxassay_001173_11	euxassay_001173_12	euxassay_001173_13	euxassay_001173_14

euxassay_001173_15	euxassay_001173_16	euxassay_001173_17	euxassay_001173_18	euxassay_001173_19

euxassay_001173_20	euxassay_001173_21	euxassay_001173_22	euxassay_001173_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11053_wholemount_strong.wlz

11053_wholemount_moderate.wlz

11053_wholemount_weak.wlz

11053_wholemount_possible.wlz

11053_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11053_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 19 20 21 22 23 not examined expression: see section 07

head mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23

dorsal grey horn

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

cervico-thoracic ganglion

weak

regional

weak expression: see section 09 13

cervical ganglion

weak

regional

weak expression: see section 08

thoracic ganglion

weak

regional

weak expression: see section 09 14 15

tongue

moderate

regional

moderate expression: see section 11 12 13 14 weak expression: see section 15 16

kidney calyx

weak

homogeneous

weak expression: see section 06 07 08 16 18

kidney pelvis

weak

homogeneous

weak expression: see section 09 17

genital tubercle of male

weak

regional

weak expression: see section 12 13 14 16

testis

weak

regional

weak expression: see section 04 05 06 07 18 19 20

axial skeleton

weak

regional

weak expression: see section 06 11 12 13 14 15

tail

moderate

regional

moderate expression: see section 11 12 13 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4651
Entity Detected:	Stc1, stanniocalcin 1 ( MGI:109131)
Sequence:	sense strand is shown >T4651 CCACGCGTCCGCTAAGCTTTCAGTATATCCAGATCCACATCTTCACTCAAGCCGGGAGAGGGAAAGAGGA AAGGGGGGGAGGAAAAAAAAAAGCCAACAACTTAGCGGAAACTTCTCAGAGAATGCTCCAAAACTCAGCA GTGATTCTGGCGCTGGTCATCAGTGCAGCTGCAGCGCACGAGGCGGAACAAAATGATTCTGTGAGCCCCA GAAAATCCCGGGTGGCGGCTCAAAATTCAGCTGAAGTGGTTCGCTGCCTCAACAGTGCCCTGCAGGTTGG CTGCGGGGCTTTTGCATGCCTGGAAAACTCCACATGTGACACAGATGGGATGTACGACATTTGTAAATCC TTCTTGTACAGTGCTGCTAAATTTGACACTCAGGGAAAAGCATTTGTCAAAGAGAGCTTAAAGTGCATCG CCAATGGGATCACCTCCAAGGTATTCCTTGCCATTCGGAGGTGTTCGACTTTCCAGAGGATGATCGCCGA GGTGCAGGAGGACTGCTACAGCAAGCTTAACGTTTGCAGCATCGCCAAGCGCAACCCGGAAGCCATCACT GAAGTCATACAGCTGCCCAATCACTTCTCCAACAGATACTACAACAGACTTGTCCGAAGCCTTCTGGAAT GTGATGAAGACACGGTCAGTACAATCAGAGACAGCCTGATGGAGAAGATCGGGCCCAACATGGCCAGCCT CTTCCACATCCTGCAGACAGACCACTGTGCCCAGACACACCCCAGAGCTGACTTCAATAGGAGGCGCACA AATGAGCCACAGAAGCTGAAAGTCCTCCTCAGGAACCTCCGAGGTGAGGGGGACTCTCCCTCACACATCA AACGCACCTCCCAAGAGAGTGCGTAAGCAGGGAGAGGTATTCACAGCCTCACCAAACTAATAGCATTTTA GGGGTGTTGACACACCAACTTTGAGTGTACTGTGCCTGGTTTGATTTTTTTTAAGTAGTACCTATTTTCT ATCCCCCCGTTAAAGAAAAATTGCATGAAACTAGGCTTCCATAATCAATATCCCAACATTCTGCAATGAC AGCATTCTTACCAACAGAATACATGTGTGGTCATTCTGCCTCTCCTCAAGAGAGAATGTACCCTCTTCCA TCCCCCCTCTCTCTGAATTCTTTTCCCAGATCCCTATCTACTCTCCGCAAACACAAACCATTCATGTAAC TACCCAGTCATTGTAATCTGAAAATGTAGATCCCTTTAGAATGGTCACCTGGTAGAGTTAGCCAATACAA AACAACTTTATTTAAATGCATGTCTTAAATGCTCATAAATATGTTAAATGGAATTCGTGTTATGAATCTG TGCTGGCCATGGACGAATATGAATGTCATGTTTGAATTCTTGATCTCTAACGAGTCTTATGGTCTCTATC CTCCAATGTCTAATTTCCTTTCTGACATATTTACCAAATTGCTCAAACCTGGTTCTCCAACCAGACTTTG AGCTAGCATCTTCTTGCATCTAATGAAAAACAAAAAAGCTAACATCTTTATGTACTGTAACTGCTCAGAG CTTTAAAAGTATCTTTAACAATTGTCTTAAAAAACGGAGAATCTTAAGGTCTAACTGTGGAATATAAATA GCTGAAAACTATTGTACTGTACATAAATTCCAGAGGACTCTGCTTAACAGAGCAGTCTATAATAACTTTA TTGCATATAGATTTAGTTTTGTACCTTAGCTTTATTTTCCTTTTCCTGGGAATGGAATAACTATCTCACT TCCAGATATCCACATTCATGCTCCTTGTGGCCTTTTTTATAACTAAGGGGGTAGAAGTAGTTTTAACTCA ACATCAGAACTTAAGATGGGCCTATACTTGACAGGAAAACCCAACAGGTTATCTGAAGGACCCCAGGTAA GACGTTAATCTCCCAGCCCACCTCAACCCGGAGGCTACGTTTGACTTAGATGTATCCTGAAACAGCTCTG TCACATCCAACTGGGAATAACAAGAATCAAAAAGACCATCCCTTTGGGCTTGGACCACTTGGTGTGACAA GGCCTACTATCCCCTTGGAAGTGGCAGTTCTTGGCTCATCGCCTTCCATCAGTGCCTGGCACTCTGGTAA ATGATGGAGTGGGATATTGTTCCACTAAGCCAATCAGGAATGAGTCAATCAGCCTTTGGGTCTTTAGTCC GGGAAACTTGGGCTTAAGGGGGTATGAATAACCCTGGGCTGTCCAGCCTTAATAGACTCCTCTTACATCT TTTGTCCTGTAACATGCTGCCTGCCAAAGTAGTCCTGGCAGCTGGACCATCTCTGTAGGATCTTTAAAAA AAAAGAAAAAAAGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5010981 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5010981 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11049 same embryo
	EMAGE:11050 same embryo
	EMAGE:11052 same embryo
	EMAGE:11051 same embryo
	EurExpress:euxassay_001173 same experiment
	MGI:4828494 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source