EMAGE mouse gene expression spatial database

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EMAGE:11615

Senp1 SUMO1/sentrin specific peptidase 1 ( MGI:2445054)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006519. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006519_01	euxassay_006519_02	euxassay_006519_03	euxassay_006519_04

euxassay_006519_05	euxassay_006519_06	euxassay_006519_07	euxassay_006519_08	euxassay_006519_09

euxassay_006519_10	euxassay_006519_11	euxassay_006519_12	euxassay_006519_13	euxassay_006519_14

euxassay_006519_15	euxassay_006519_16	euxassay_006519_17	euxassay_006519_18	euxassay_006519_19

euxassay_006519_20	euxassay_006519_21	euxassay_006519_22	euxassay_006519_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11615_wholemount_strong.wlz

11615_wholemount_moderate.wlz

11615_wholemount_weak.wlz

11615_wholemount_possible.wlz

11615_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11615_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6599
Entity Detected:	Senp1, SUMO1/sentrin specific peptidase 1 ( MGI:2445054)
Sequence:	sense strand is shown >T6599 GGATTCTGTGTCTTTACTAAAGCCAGTGATGTGAAGAGACCTGAAATGGATGACACAGCTGATGGGGTGA AGATGGACGCTGGGGAGGTGACCTTAGTGAACCACGGCTCCACTTTCAGAACCCACCGCCCGCCACAGTC AGGCTTTCCAGAGGAGCAGCTTCTGCTTTCTGACCAGCAGAGCTTGCCTTTCAGGCAGGGTACTTTAGAT GGATCTTTCACGTGTTCTACAAGAAGCCCAGCCTATCGTCCAGATTATCACTCAGATAACCCTTCCTCAG ACAGTTTCCTTGGCTCAGGTGACGTAAGAACCTTTGGTCAAAGTGCAAATGGACAATGGAGAAATTCCAC TCCAGCGTCAGGCTCAGCGCCACAAAAACCAAGAAATAGCCGAAGTCTTTGCCTCGAAACCCGGAAGACC TCAAGTGGATTATCAAACACCTTTGTGGGCAAGTCCAATCACCACTGCCACATGTCTGCGTATGAAAAGT CATTTCCCATTAAGCCTGCCCCAAGTCCATCCTGGAGTGGGTCATGTCGTCGGAGTCTTTTAAGCCCTAA GAAAACTCAGAGGCGACATTTCAGCACAGCAGAAGAGACAGTTCAAGAAGAAGAGAAGGAGATCTACAGA CAGCTGCTGCAGATGGTCACAGGGAAGCAGTTCTGTGTAGCGAAACCCACCACACATTTTCCGTTACGCC TGTCTCGGTGTCTTAGTTCCAATAAGAACTCTTTGAAAGACTCGCTGTTGAGAAACGGAAACTCTTGTGC ATCTCATGTCATTGGTTCTGATACGTCATCCTCTGGATCTGCCAGCATTTTAACTGCACAGGAGCAGTTG TCCCACAGTGCACATTCCCTGTCATCTGGTACCCCAGATGTTGCCTTTGGATCCAAAGACTCTGATCCTC ACCATCACCTCGCTGCACCTCATCAGCCAAATAGCTTACCAGCATCAAATACACAGTCCGAAGGGTCAGA CTCTGTGATTTTACTTAAAGTGAAAGAATCTCAGACTCCAGCTTCCAGCCCTACGTTCTTCCAGGCAGAG CTATGGATCAAAGAATTAACTAGCGTTTATGACTCTCGAGCTCGGGAAAGATTGCGCCGGATTGAAGAGC AGAAAGCATTGGCACTACAGCTTCAAAACCAGCGACTGCAGGAGCAGGAACATGCAGTACTTGACTCCGT AGAGCTTCACCTTCGTGTGCCTCTTGAAAAGGAGATTCCTGTCACAGCTGCCCAAGAAACCAGGAAAAAA AGCCACCAGCTGACTGACAGTGAAGACGAATTTCCTGAAATTACAGAGGAAATGGAAAAAGAAATAAAGA ATGTATTTCGTAACGGTAACCAGGATGAAGTCCTCAGTGAGGCATTCCGCTTGACCATTACACGCAAAGA CATTCAGACTCTAAACCATCTAAACTGGCTCAATGATGAGATCATCAATTTCTACATGAACATGCTAATG GAACGCAGTAAAGAGAAGGGGTTTCCAAGTGTACATGCGTTTAATACCTTCTTCTTCACTAAGCTAAAAA CAGCTGGTTATCAGGCAGTGAAACGCTGGACAAAGAAGGTGGATGTATTCTCCGTTGATATTCTTCTGGT GCCCATTCACTTGGGTGTGCACTGGTGTCTAGCTGTTGTAGACTTTAGAAGGAAGAGTATCACCTACTAT GATTCTATGGGTGGAATAAACAACGAGGCCTGCAGGATCCTCTTGCAATACCTAAAACAAGAAAGTGTTG ACAAGAAAAGGAAAGAGTTTGACACCAACGGCTGGCAGCTCTTCAGCAAGAAGAGCCAGGAAATCCCACA GCAGATGAATGGGAGTGACTGTGGAATGTTTGCTTGCAAATATGCTGACTGCATTACCAAAGACAGACCA ATCAACTTCACACAGCAACACATGCCATATTTCCGAAAGCGAATGGTCTGGGAGATCCTCCACCGGAAGC TCTTGTGAAGATTCTCAGTGAGCAGACACTGAGCGTGTGGGGGACCAACCTTTGCCATCGACAGCCGGAG ACCTTGGAAAGAGCTGCTCCTCCCTCCCCTGTTCTTGTACAGCCCTTGACCCTGGACCAGGCCCGGCGAG ATGCATTCACAAGCACATCTGCCTTCCCTTTTGTAGCTCAGATCCTATTTTTGCAAAGAAACTTTGGTGC TGTGAAGGGGTGAGGGAACATCCCTCCTGAAGAGACTGCCTTTCACTTCAGCTCTGCCGTCTTCTTTTCC AAGGGCTCCCGCTCACTGAGTCCCTCACTAGGAGCCCAAGAGAGCCTCGGGAAGAATCTCGGTTTCCTGT AAACCCTTGTTGCTCGGCCTTACCGAGAAGCAGGAAAGAGCATGGGCAGCAGGTAGGGGTAAAACCACCA GCATGTAGGACACACATCCCCAAGACGCAGAGTCGGCAATGGAGCTGCTTAAGTCCCTCCTCCAGGACCT TCCTATTTATGTGCCCTCCATGACCGACTGCTTCTCCTCCAAGCTGTTTCTTCACTGGTAGCGTCATCCC ATCCTAAGCACACTACACAAGCCCTGGCAGACCCTAAAGTCTCGAGCAGTCCGTTCCTTACAAGCAGGGC TGCATGTGCGAGCACGCACGTGCTGTGGGAGGGCCCCCAGAAGTACTGAGCGCAGCAGCCGGGCCCCAGC TTCAGTGCTGCCTGCTTCTGACCAAGAAGAAGGACCTTGGCGGTTAGCATCACACGCCAGAGTAGGATGA ATACAGGTCTAGGTGGTCTGTGGCTAGTTAGTTTAAATATGCTTCTAGCCTCAGAGAGTTTTATATACAT ATATGCCTATATGTGTGTATACATACATATATACACACACACACACAATGTGAAATATATATTTCACAGG GATATGTTTTTAAAAGTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3595534 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3595534 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11614 same embryo
	EMAGE:11617 same embryo
	EMAGE:11616 same embryo
	EMAGE:11613 same embryo
	EMAGE:11612 same embryo
	EurExpress:euxassay_006519 same experiment
	MGI:4827951 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source