EMAGE mouse gene expression spatial database

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EMAGE:11741

Pacsin1 protein kinase C and casein kinase substrate in neurons 1 ( MGI:1345181)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007123. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007123_01	euxassay_007123_02	euxassay_007123_03	euxassay_007123_04

euxassay_007123_05	euxassay_007123_06	euxassay_007123_07	euxassay_007123_08	euxassay_007123_09

euxassay_007123_10	euxassay_007123_11	euxassay_007123_12	euxassay_007123_13	euxassay_007123_14

euxassay_007123_15	euxassay_007123_16	euxassay_007123_17	euxassay_007123_18	euxassay_007123_19

euxassay_007123_20	euxassay_007123_21	euxassay_007123_22	euxassay_007123_23	euxassay_007123_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11741_wholemount_strong.wlz

11741_wholemount_moderate.wlz

11741_wholemount_weak.wlz

11741_wholemount_possible.wlz

11741_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11741_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

adenohypophysis

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24

vagus x ganglion

moderate

regional

moderate expression: see section 08 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 18 19 20

spinal cord

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 16 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20 21 22 23

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 16 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4359
Entity Detected:	Pacsin1, protein kinase C and casein kinase substrate in neurons 1 ( MGI:1345181)
Sequence:	sense strand is shown >T4359 CTCCTCCTCCTTTTCCTCCTCCTCCCCCGGGAAGCCGGCCGGGCTTGAGGCTGGGCCGCCGCCGCCGCCC GCTTTGCCACCCGCCCCGCTGATGGTGTCCGGTGCTCCGGCGCCCAGCCTGTGAGCAGCAGGCCTGCTAA CTGCAGATCCACAACCGCACAGCTCGCTACAGGTGCACCATGTCTGGCTCCTACGATGAGGCCTCAGAGG AGATCACAGATAGCTTCTGGGAGGTGGGGAACTACAAGCGGACGGTGAAGCGCATCGACGATGGGCACCG CCTGTGCAACGACCTCATGAGCTGCGTGCAGGAGCGCGCCAAGATCGAGAAGGCATACGCGCAGCAGCTC ACCGACTGGGCCAAGCGCTGGCGCCAGCTCATCGAGAAAGGTCCTCAGTATGGCAGCCTGGAGCGGGCGT GGGGCGCCATGATGACAGAAGCAGATAAGGTCAGCGAGCTGCACCAGGAGGTGAAGAACAGCCTGCTGAA TGAGGACCTGGAGAAAGTCAAGAACTGGCAGAAGGATGCCTATCACAAGCAGATCATGGGTGGCTTCAAG GAGACGAAAGAGGCCGAGGATGGCTTCCGAAAGGCCCAGAAGCCCTGGGCTAAAAAGATGAAGGAGCTAG AGGCGGCCAAGAAGGCCTATCACTTGGCTTGTAAGGAGGAAAGGCTGGCCATGACCCGGGAGATGAACAG TAAGACAGAGCAGTCGGTCACCCCTGAACAGCAGAAGAAACTTGTGGACAAAGTGGACAAATGCAGACAG GATGTGCAAAAGACTCAGGAGAAGTATGAGAAGGTGCTGGAAGATGTGGGCAAGACCACACCACAGTACA TGGAGGGCATGGAGCAGGTGTTTGAGCAGTGCCAGCAGTTTGAGGAGAAGCGGCTGGTCTTCCTGAAGGA AGTCCTGCTGGATATCAAACGGCATCTCAACCTAGCGGAGAACAGCAGCTACATGCATGTCTACCGAGAA CTGGAGCAGGCCATCCGGGGGGCCGATGCCCAGGAGGACCTCAGGTGGTTCCGCAGCACCAGTGGCCCCG GGATGCCCATGAACTGGCCGCAGTTCGAGGAGTGGAACCCAGACCTCCCGCACACCACTGCCAAGAAGGA GAAACAGCCTAAGAAGGCAGAGGGGGCCACCCTGAGCAATGCCACTGGGGCTGTAGAATCCACATCCCAG GCTGGGGACCGTGGCAGTGTTAGCAGCTATGACCGAGGCCAAACATATGCCACCGAGTGGTCAGACGATG AGAGCGGAAACCCCTTCGGGGGCAATGAGGCCAATGGTGGCGCCAACCCCTTCGAGGATGATGCCAAGGG AGTTCGTGTACGGGCACTCTATGACTACGACGGTCAGGAGCAGGATGAGCTCAGCTTCAAGGCCGGAGAT GAGCTCACCAAGCTCGGAGAGGAAGACGAACAGGGTTGGTGCCGCGGGCGGCTGGACAGCGGACAGCTGG GCCTCTATCCTGCCAACTACGTTGAGGCTATATAGCTACCTTGCCCACCCGACTCCTCTCAGTCCTTGTC CACCGCCTTCCACCCTTCCCCTCCCCCTTGCCATAGAGTTCCAGACATATTTTCCGATCAAGCTTTTATT TTTTTAAAAGTCAAAACAGAACAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4527708 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4527708 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11743 same embryo
	EMAGE:11740 same embryo
	EMAGE:11742 same embryo
	EMAGE:11738 same embryo
	EMAGE:11739 same embryo
	EurExpress:euxassay_007123 same experiment
	MGI:4827023 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source