EMAGE mouse gene expression spatial database

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EMAGE:11782

Dnahc9 dynein, axonemal, heavy chain 9 ( MGI:1289279)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010961. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010961_01	euxassay_010961_02	euxassay_010961_03	euxassay_010961_04

euxassay_010961_05	euxassay_010961_06	euxassay_010961_07	euxassay_010961_08	euxassay_010961_09

euxassay_010961_10	euxassay_010961_11	euxassay_010961_12	euxassay_010961_13	euxassay_010961_14

euxassay_010961_15	euxassay_010961_16	euxassay_010961_17	euxassay_010961_18	euxassay_010961_19

euxassay_010961_20	euxassay_010961_21	euxassay_010961_22	euxassay_010961_23	euxassay_010961_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11782_wholemount_strong.wlz

11782_wholemount_moderate.wlz

11782_wholemount_weak.wlz

11782_wholemount_possible.wlz

11782_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11782_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon roof plate

moderate

regional

moderate expression: see section 14 15

choroid invagination

moderate

regional

moderate expression: see section 07 08 09 10 11 12 16 17 18 19

medulla oblongata floor plate

strong

regional

strong expression: see section 12

metencephalon floor plate

strong

regional

strong expression: see section 12

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

spinal cord floor plate

strong

regional

strong expression: see section 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36231
Entity Detected:	Dnahc9, dynein, axonemal, heavy chain 9 ( MGI:1289279)
Sequence:	sense strand is shown >T36231 TATCACAGATGACTGGGACAGGAGACTCTGCAGAACCTATTTAGAGGAATTCATTCGGCCAGAGATGCTA GAAGGAGAGCTCTCCCTGGCCCCGGGGTTCCCACTCCCAGGAAACATGGACTACAGTGGCTATCACCAGT ATATTGATGCTGAGCTGCCCCCTGAGTCTCCCTACCTATATGGCCTCCATCCAAACGCCGAGATTGGCTT CCTGACCCAGACATCGGAAAAACTCTTCCGAACCGTGCTGGAGATGCAGCCTCGGGACAGCCAGGCTGGA GATGGAGCTGGCATCACAAGGGAAGAAAAGGTCAAAACCTTTCTGGAAGAAATACTGGATCGGATGACAG ATGAATTTAACATCGCGGAGCTAATGGCTAAGGTGGAGGAACGCACCCCCTACATTGTAGTTGCCCTCCA GGAGTGTGAACGCATGAACATCCTTACCAGAGAGATCCAGCGCTCACTAAGAGAGCTGCATCTTGGCTTA CAGGGGGAGCTGACCATGACCAGTGAGATGGAAAACCTACAGAATGCCCTATATCTAGATGTGGTCCCAG AGTCCTGGGCCAGGCGAGCCTACCCTTCCACAGCAGGCCTGGCAGCCTGGTTTCTAGACCTGCTTAACAG AATCAAAGAGCTGGAGTCCTGGACAGGCGACTTCTTGATGCCCTCAACTGTGTGGCTGACGGGCTTCTTC AACCCCCAGTCCTTCCTGACTGCCATCATGCAGTCCATGGCCCGCAAGAATGAATGGCCACTAGACCAGA TGGCCCTGCAGTGTGATGTGACAAAGAAGAACAGAGAGGAGTTCCGGAGCCCTCCTCGGGAAGGGGCCTA CATCTATGGGCTCTTCATGGAAGGTGCCTGCTGGGACACACAGACTGGGATCATTGCAGAGGCGAAACTG AAGGACCTGACACCCCCCATGCCTGTGATGTTCCTCAAGGCCATT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 87416. Forward Primer - name:087416_F_cDNA_D11Ertd686e, sequence:TATCACAGATGACTGGGACAGG; Reverse Primer - name:087416_N_SP6_cDNA_D11Ertd686e, sequence:AATGGCCTTGAGGAACATCAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11785 same embryo
	EMAGE:11784 same embryo
	EMAGE:11781 same embryo
	EMAGE:11783 same embryo
	EurExpress:euxassay_010961 same experiment
	MGI:4824342 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source