EMAGE mouse gene expression spatial database

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EMAGE:11795

Plin3 perilipin 3 ( MGI:1914155)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007119. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007119_01	euxassay_007119_02	euxassay_007119_03	euxassay_007119_04

euxassay_007119_05	euxassay_007119_06	euxassay_007119_07	euxassay_007119_08	euxassay_007119_09

euxassay_007119_10	euxassay_007119_11	euxassay_007119_12	euxassay_007119_13	euxassay_007119_14

euxassay_007119_15	euxassay_007119_16	euxassay_007119_17	euxassay_007119_18	euxassay_007119_19

euxassay_007119_20	euxassay_007119_21	euxassay_007119_22	euxassay_007119_23	euxassay_007119_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11795_wholemount_strong.wlz

11795_wholemount_moderate.wlz

11795_wholemount_weak.wlz

11795_wholemount_possible.wlz

11795_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11795_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

strong

regional

strong expression: see section 07 08 09 10 11 15 16 17 18 19 20 moderate expression: see section 06 12 13 14

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 07 08 09 10 11 15 16 17 18 19 20 21 22 moderate expression: see section 03 04 05 06 12 13 14 23

nasal septum

weak

regional

weak expression: see section 16

viscerocranium

weak

regional

Expression in the turbinate bone.

tongue muscle

moderate

regional

moderate expression: see section 14 15 weak expression: see section 10 11 12 16

pharynx vascular element

moderate

regional

moderate expression: see section 13

stomach

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10

midgut

strong

regional

strong expression: see section 14 15 16 17 18 19 20 21 moderate expression: see section 04 05 06 07 08 09 10 11 12 13

bladder

weak

regional

weak expression: see section 13 14 15

metanephros

weak

regional

weak expression: see section 09 10 11 20 21

urethra of male

weak

regional

weak expression: see section 13 14 15

nucleus pulposus

moderate

regional

moderate expression: see section 15 weak expression: see section 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7162
Entity Detected:	Plin3, perilipin 3 ( MGI:1914155)
Sequence:	sense strand is shown >T7162 CCAAGCTGGACTTGGTAGGGGAGGTTTCCTCGCTTGGACGACCTGAGGACTTTGCAACTGCTGCTGAGAC CATGTCTAGCAATGGTACAGATGCGCCTGCCGAAGCTCAAGCTGCTATGGAGGAACCTGTTGTGCAGCCC AGCGTGGTGGATCGTGTTGCCGGCCTGCCTCTTATCAGTTCCACGTATGGGATGGTGAGCGCCGCCTACA CCTCCACCAAGGAGAACTACCCCCACGTCAGGACTGTGTGCGATGTGGCTGAGAAAGGCGTCAAGACCCT CACCACGGCCGCTGTCAGCACAGCACAGCCCATCCTGTCCAAGCTGGAGCCCCAGATTGCGACGGCCAGT GAGTATGCACACCGAGGGCTGGACAGACTGCAGGAAAGCCTGCCCATCCTCCAGCAGCCGACTGAGAAGG TTTTGGCGGATACTAAGGAACTGGTGTCATCTACAGTGTCTGGGGCTCAAGAAATGGTATCTAGCTCAGT GTCTAGTGCGAAGGAAACAGTGGCCACCCGGGTCACAGGAGCGGTGGATGTGACCCTCGGGGCTGTGCAG AACAGCGTGGACAAGACCAAGTCAGCAATGACCAGCGGTGTCCAATCAGTCATGGGCTCACGTGTGGGAC AGATGGTGATTAGCGGAGTGGACAGAGTGCTTGTGAAATCAGAGGCCTGGGCAGACAATCGCCTGCCCCT GACAGAGGCGGAGCTAGCTCTGATTGCCACACCCCCGGAGGACTCGGACATGGCCTCACTGCAGCAGCAG CGACAGGAGCAGAACTACTTTGTCCGGTTGGGCTCTCTCTCTGAGAGGCTGCGCAATCACGCCTATGAAC ACTCCCTCGGCAAGCTGCAGAATGCCAGGCAGAAGGCACAGGAGACGCTGCAGCAGCTAACAAGTGTGCT CGGCCTGATGGAATCCGTGAAACAGGGTGTGGACCAGAGGCTGGGGGAGGGGCAGGAGAAGCTGCACCAG ATGTGGCTCAGCTGGAACCAGAAGACCCCACAGGATGCTGAAAAGGACCCAGCTAAGCCAGAGCAGGTGG AGGCCCGGGCACTCAGCATGTTCAGGGACATCACCCAGCAGCTGCAGAGCATGTGTGTGGCCCTGGGGGC CAGCATCCAGGGCTTGCCCAGCCACGTCAGGGAGCAGGCACAGCAGGCCCGCAGCCAGGTGAACGACCTT CAGGCCACCTTCTCCGGCATTCACTCCTTCCAGGACCTCTCAGCCGGCGTTCTTGCCCAAACTCGGGAGC GCATAGCCAGAGCCCGGGAGGCCCTAGACAACACTGTGGAGTACGTGGCCCAGAACACTCCAGCCATGTG GCTGGTGGGCCCCTTCGCTCCTGGAATCACAGAGAAAACCCCTGAAGGGAAGTAGGGAGAAGGTTCAGGG CCAACATCTATCTGGCTTGACTGCTTTTCCCAACAGTCCAATTGCACAACAGGTCCTCCCCTACTGAGTC TGAATGAGACATGTGTTTAACCTATTCCCATCAGTCTTTTAAAGCAATTTTTTTTCTAGAATGTTCCAGG GGTAAAAAGATCCAGAACATTCTAGAAAAAAAAAATGTTTTTTTCCTAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:4023354 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4023354 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11793 same embryo
	EMAGE:11797 same embryo
	EMAGE:11798 same embryo
	EMAGE:11792 same embryo
	EMAGE:11796 same embryo
	EMAGE:11794 same embryo
	EurExpress:euxassay_007119 same experiment
	MGI:4827272 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source