EMAGE mouse gene expression spatial database

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EMAGE:11828

Ndfip2 Nedd4 family interacting protein 2 ( MGI:1923523)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007122. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007122_01	euxassay_007122_02	euxassay_007122_03	euxassay_007122_04

euxassay_007122_05	euxassay_007122_06	euxassay_007122_07	euxassay_007122_08	euxassay_007122_09

euxassay_007122_10	euxassay_007122_11	euxassay_007122_12	euxassay_007122_13	euxassay_007122_14

euxassay_007122_15	euxassay_007122_16	euxassay_007122_17	euxassay_007122_18	euxassay_007122_19

euxassay_007122_20	euxassay_007122_21	euxassay_007122_22	euxassay_007122_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11828_wholemount_strong.wlz

11828_wholemount_moderate.wlz

11828_wholemount_weak.wlz

11828_wholemount_possible.wlz

11828_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11828_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 15 16 17 18

pancreas

moderate

regional

moderate expression: see section 08 09 10 14 15 16

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 08 09 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 17 18

trigeminal v nerve

strong

regional

strong expression: see section 09 16 moderate expression: see section 10

spinal cord

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 15 16 17

cornea epithelium

moderate

regional

moderate expression: see section 04 22 23 weak expression: see section 02 03

not examined

regional

not examined expression: see section 01 02 03 22 23

neural retina

strong

regional

strong expression: see section 01 02 03 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 18 weak expression: see section 08 09 10 11 13 14 15 16 17

nasal cavity respiratory epithelium

weak

regional

weak expression: see section 11 12 14 15

hindgut

moderate

regional

moderate expression: see section 14 weak expression: see section 12 13

rectum

weak

regional

weak expression: see section 12

midgut

moderate

regional

moderate expression: see section 14 15 16 17 18 weak expression: see section 07 08 09 10 11 12 13

bladder

weak

regional

weak expression: see section 12 13

urethra of male

weak

regional

weak expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4619
Entity Detected:	Ndfip2, Nedd4 family interacting protein 2 ( MGI:1923523)
Sequence:	sense strand is shown >T4619 CGCCGATCGGCGAGCGACGCGGAGCTCTCCGCTGGAGCCGAGGGAGCGACAGGAAGTGAAGCGGCCCCGC CAGGCGACCTCGGCGGCAGAACCCGAGGCGGCGGAAGAGGCTCTGCGGCGGCGGCGGCGACGACGTCGAC CCGGGAGGCCGAGGGAGCTGAGCGCCGAGGGGACACGCCCGCTCGGAAGCCGGACCCCGAGGCGGGCAGG ATGGATCACCACCAGCTGGGGACTGGTCGCTACCAGGTGCTTCACAATGAAGAAGATAACTCAGAATCAT CTGCTGTGGAGCAGCCGTCAACTTCCAGCTTAGCAGCGCCGACTGTGGAGGCTGCCACTTCAGCACCAGC ACTGGACCCTGACTCTCCTCCTCCTTACAGCAGCATCACTGTGGAAGCTCCTACCACTTCAGATACTGAC GTGTATAGTGAGTTTTACCCTGTGCCACCTCCGTACAGTGTTGCTACCTCTCTGCCTACATATGACGAAG CTGAAAAGGCTAAAGCTGCTGCCCTGGCAGCTGCTGCAGCAGATGCACCTCAGAGAAACCAAGAGGAAGA CTGTACGCCACGGGATGACTTCAGTGATGTAGAGCAGCTCAGAGTGGGCAACGACGGCATTTTCATGCTG GCCTTTTTCATGGCATTCATATTCAACTGGCTTGGGTTCTGCTTATCCTTCTGTATCACCAATACCATCG CTGGAAGATACGGTGCTATCTGTGGGTTTGGCCTTTCACTGATCAAATGGATCCTTATTGTCAGGTTTTC TGACTATTTTACTGGCTATTTCAATGGACAATATTGGCTTTGGTGGATATTTCTCGTACTTGGCCTGCTC CTTTTCTTCAGAGGGTTTGTTAATTACCTAAAAGTCAGAAACATGTCTGAAAGTATGGCGGCTGCACATA GGACGAGGTACTTCTTCCTGCTATAGAGGTGGCATCAACCCATCATTCCTTTCTTACACCAATGTGAACC GTCCACATCATCTGTAAAGCGACAGCTTTGCTAAGATAGAAGACTGCTGAGAGCAGAAGGAAATGTAGTG AAGGGTGGAGCTTCGGAATTAAATGACAGGGTGGTTTACGCTAAAAGCCATTTCTGTTCATTCCTTAATC TGTGTATTTCATTTATTTTGCACATTTGCATATGTGCTTGTCTTACAGATATGCATATACTTGAAAGAAT CTATAACGTTGTAGCAGCAGAGCCCAGTCCTGCTCGGTTACTCAGTGCTGATACAATTGGAGGAATTGGG TAACGAGCAGCCTTCCAGCACGTGACGCTTCCGACGTGGCCGTTAGCATAATCAGAGTTACACTATTACT CTTGTCCAGTGCTTTACTTTCTGCCTCTTAGATGCATCTAGTAATCCACACACGAAATATTCTTTGTTAT ATATGATGTTTATTAAAACTTGTGGTGTGCTTATTAGAAAGAATTTGTCAGATTTTTGAATATGATATTA TTTAGGAAATCTGTAAATAACCATGCATATATTGCTTTTTACATTGTTTTCTTAAATTGTGTCTAGATAT CTTTTCATTAATTTTAAATTAAGTAAACTAAATAAATAAAAATAAACTTTTTTAAAGATGTGTGGTTTAG GATGGGCTTTAAGAAAGTGAGTCTACCAAATGCCAGTTAAAGAGCAAGTTAGGGGTACGCCAGTTTCAGG GGTGTTCAGATGAGAACCTTAGGTTTTAAGATTACTCACCTTACAGTTCTTATAAGAAGAGATTGCTTTA GATCTTATATTTATTTACATTTAAAACAATTTAAAAATTAATTTACCAAATATATCCTCATTTGCATTTG TCTTTTAAAAAGCCAATAAAGATATCTTTCAGAGTATTACTACAATCATTTTTCTTTAAATGTTTAATTT GTTAAGTGTAGAAAATAAAATCTTGTTCTGTTAATAGTGCCTCGTTTAGTGATTCCAAGACTAATTTGTA AAAACGTACAGCCTGAATTGAGATCCTCAGAATACCTGATTTCATGTTGTTGAAATGTCTCTATAGAATG AATTTAGGGTGTGGAGTGTGTGTGGTGAGTGTGTGTGACCATTACTCCAACTGGAATCTGCTTTTACATC AAAAGCTACAATGCTAGCCATCTCACAGATCATTTTCTGACTTTTGTGGTTTTCATTTTATCTTAACTTA TGAATGCTAAAAGTAAAAGATGATGGAAATTACACTGCTGCTTACATATAAAAAAAAACATTTAACATAG CCGTAAAGAACATACAAAATTGATTGCAACACTATGACTAAAAAGTGCATCTAACTCTTCAGGGCTGTTT GTCCCATCTTAAGGAAAAATAAAATTTATTACTTTATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5003738 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5003738 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11826 same embryo
	EMAGE:11825 same embryo
	EMAGE:11829 same embryo
	EMAGE:11827 same embryo
	EMAGE:11824 same embryo
	EurExpress:euxassay_007122 same experiment
	MGI:4826629 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source