EMAGE mouse gene expression spatial database

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EMAGE:11880

Mast4 microtubule associated serine/threonine kinase family member 4 ( MGI:1918885)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011099. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011099_01	euxassay_011099_02	euxassay_011099_03	euxassay_011099_04

euxassay_011099_05	euxassay_011099_06	euxassay_011099_07	euxassay_011099_08	euxassay_011099_09

euxassay_011099_10	euxassay_011099_11	euxassay_011099_12	euxassay_011099_13	euxassay_011099_14

euxassay_011099_15	euxassay_011099_16	euxassay_011099_17	euxassay_011099_18	euxassay_011099_19

euxassay_011099_20	euxassay_011099_21	euxassay_011099_22	euxassay_011099_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11880_wholemount_strong.wlz

11880_wholemount_moderate.wlz

11880_wholemount_weak.wlz

11880_wholemount_possible.wlz

11880_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11880_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 13 14 15 moderate expression: see section 11 12

hypothalamus mantle layer

strong

regional

strong expression: see section 08 09 10 14 15

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 moderate expression: see section 06 18

telencephalon mantle layer

strong

regional

strong expression: see section 09 17

olfactory cortex marginal layer

weak

regional

weak expression: see section 10 11 12 13 16 17 18

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 06 07 08 09 11 12 13 14 15 16 17

rest of cerebellum mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 16 17 18 19

pons mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

ventral grey horn

strong

regional

strong expression: see section 17 moderate expression: see section 13 14 15 16 weak expression: see section 08 09 10 11 12 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37361
Entity Detected:	Mast4, microtubule associated serine/threonine kinase family member 4 ( MGI:1918885)
Sequence:	sense strand is shown >T37361 AATGGTCCTTGTCTCTGGGTTATATGTCTGGTCTTCACCGGAGGGCACACAGGGACTATAAACACACTGC AGGTCAGCTTTCTTTCTTTCTCTCCTTCCTTCCTTTCTAAGCTCTACCCTCAGGCCTTTATGCTGTGAGT CCAAGAAGCACATATTCGTGGCAGGCACTGGACCAGCGCTGCTGGTGAGGAAACAGGCAAGTCCCTGGCT GCACACAAATCCGCTAGCACTTAGGTGGAGCGATACGCATTAGGCATTGGAAGAGGCTTTAACTTTGCTT TGGTTGAAGAAAAGGAGGGGGGTGGATTTTGGCATCATAGCATATATATTAAAGAATAATCAGGACATCA GATACATTTTAATACATAGCTGGGGCCTTATGTTGTAGATGAAGCTTGCTGTTTTTAGCAAGTTCCTGGG TTTCACATTCATTTCTGATGCATCGAGTAGCTCCATGCATTTCATAACACAATCTCTTTATTTGTATGCA AAAAATTTACTGTATTAATAAAGAGCATCATTTTGTAACAAAGAGACTGGAGCTATTTGGTGCTTGAATG TGAACATCCTTTTTACTTTTGCTAAGCCTTATTTAAATTTTGTATACCAGGAAATATGTAGAACTTGTCA GATCATTTTAGCTCTGAGGGTTTTTCTGGGTTGTTTCTGTTGTTGCGATGGTTTTTTTATGTTTTGTTTT GTTTTGTTTCGTTGGTGGGTTTGGGTTTTTGTTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 84356. Forward Primer - name:084356_F_cDNA_4930420O11Rik, sequence:AATGGTCCTTGTCTCTGGGTTA; Reverse Primer - name:084356_N_SP6_cDNA_4930420O11Rik, sequence:AAACAAAAACCCAAACCCACC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11881 same embryo
	EurExpress:euxassay_011099 same experiment
	MGI:4826108 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source