Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11902

Itga2 integrin alpha 2 ( MGI:96600)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11902 EMAGE:11902 EMAGE:11902 EMAGE:11902 EMAGE:11902
"Pseudo-wholemount" of euxassay_009582. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009582_01 euxassay_009582_02 euxassay_009582_03 euxassay_009582_04
EMAGE:11902 EMAGE:11902 EMAGE:11902 EMAGE:11902 EMAGE:11902
euxassay_009582_05 euxassay_009582_06 euxassay_009582_07 euxassay_009582_08 euxassay_009582_09
EMAGE:11902 EMAGE:11902 EMAGE:11902 EMAGE:11902 EMAGE:11902
euxassay_009582_10 euxassay_009582_11 euxassay_009582_12 euxassay_009582_13 euxassay_009582_14
EMAGE:11902 EMAGE:11902 EMAGE:11902 EMAGE:11902 EMAGE:11902
euxassay_009582_15 euxassay_009582_16 euxassay_009582_17 euxassay_009582_18 euxassay_009582_19
EMAGE:11902 EMAGE:11902 EMAGE:11902 EMAGE:11902 EMAGE:11902
euxassay_009582_20 euxassay_009582_21 euxassay_009582_22 euxassay_009582_23 euxassay_009582_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11902Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11902_wholemount_strong.wlz
11902_wholemount_moderate.wlz
11902_wholemount_weak.wlz
11902_wholemount_possible.wlz
11902_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11902_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
adrenal medulla
weak weak
regionalweak expression: see section 09 10 11 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36524
Entity Detected:Itga2, integrin alpha 2 ( MGI:96600)
Sequence:sense strand is shown

>T36524
GTGCATACAACACTGTGGTCCTGGCCGAGTTTTCTGAGAACTTGTTTTTTGCTTCTTTCTCCATGCCGGT
CGATGGAACAGAAGTAACATGTGAGGTTGGTTCATCGCAGAAATCTGTTACCTGTGATGTGGGATACCCT
GCCTTAAAGAGTGAACAGCAGGTGACTTTTACCATTAACTTCGACTTTAATCTTCAAAACCTCCAGAACC
AGGCGGCCATCAATTTCCAAGCCTTCAGTGAGAGCCAAGAAACAAACAAAGCAGACAATTCGGTCAGTCT
CACGATTCCTCTCCTGTATGATGCCGAACTTCACTTAACCCGATCCACCAACATCAACTTCTATGAGATC
TCTTCGGATGAGAACGCTCCTTCTGTCATCAAGAGTGTCGAAGATATTGGTCCTAAATTCATCTTCTCCC
TCAAGGTAACAGCCGGAAGTGCCCCAGTGAGCATGGCATTGGTGACTATCCACATTCCTCAGTACACCAA
GGAAAAGAACCCACTCCTGTATCTGACTGGGATACAAACAGACCAGGCTGGCGACATCAGCTGCACAGCA
GAAATAAATCCACTCAAGCTACCACACACCGCCCCTTCTGTATCTTTCAAGAATGAAAACTTCCGGCATA
CGAAAGAATTGGACTGCAGAACCACTTCCTGCAGTAACATCACCTGCTGGCTGAAAGACCTTCACATGAA
AGCAGAGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96504. Forward Primer - name:096504_F_cDNA_Itga2, sequence:GTGCATACAACACTGTGGTCCT; Reverse Primer - name:096504_N_SP6_cDNA_Itga2, sequence:ACTCTGCTTTCATGTGAAGGTCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11903 same embryo
 EurExpress:euxassay_009582 same experiment
 MGI:4825639 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS