EMAGE mouse gene expression spatial database

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EMAGE:11953

Map3k5 mitogen-activated protein kinase kinase kinase 5 ( MGI:1346876)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011097. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011097_01	euxassay_011097_02	euxassay_011097_03	euxassay_011097_04

euxassay_011097_05	euxassay_011097_06	euxassay_011097_07	euxassay_011097_08	euxassay_011097_09

euxassay_011097_10	euxassay_011097_11	euxassay_011097_12	euxassay_011097_13	euxassay_011097_14

euxassay_011097_15	euxassay_011097_16	euxassay_011097_17	euxassay_011097_18	euxassay_011097_19

euxassay_011097_20	euxassay_011097_21	euxassay_011097_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11953_wholemount_strong.wlz

11953_wholemount_moderate.wlz

11953_wholemount_weak.wlz

11953_wholemount_possible.wlz

11953_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11953_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 09 10 11 12 13

thyroid gland

weak

regional

weak expression: see section 09 13 14

vibrissa

weak

regional

weak expression: see section 04 05 06 20 21 22

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 07 14 weak expression: see section 15

trigeminal v ganglion

strong

single cell

strong expression: see section 06 07 16 moderate expression: see section 04 05 17 18 19 20 21 weak expression: see section 03

lens

weak

regional

weak expression: see section 01 02

naris

moderate

regional

moderate expression: see section 11 weak expression: see section 12 15

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 15 weak expression: see section 07 08 09 10 12 13 16 17 18

bladder

weak

regional

weak expression: see section 11 12 13

clavicle

weak

regional

weak expression: see section 07 08 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36616
Entity Detected:	Map3k5, mitogen-activated protein kinase kinase kinase 5 ( MGI:1346876)
Sequence:	sense strand is shown >T36616 GAGAAAGGATAGTGAGAGGCGAGCTACCCTTCACAGAATCCTGACCGAAGACCAGGACAAGGTTGTAAGG AACCTAATGGAATCTCTGGCCCAGGGTGCTGAAGAGCCTAAACTAAACTGGGAACACATCACAACCCTCA TCTCAAGCCTCAGAGAGTTTGTGAGGTCCACTGACCGAAAAATCATAGCCACTACATTGTCCAAGCTCAA GCTGGAGCTGGACTTCGACAGCCACGGCATCAGCCAGGTGCAGGTGGTCCTCTTTGGCTTCCAGGATGCG GTCAATAAAGTTCTTCGGAATCATAACATCAAGCCACACTGGATGTTTGCCCTGGACAGCATCATCCGAA AGGCTGTGCAGACTGCAATTACCATTCTGGTTCCAGAGTTGAGGCCACATTTTAGCCTTGCATCAGAGAG CGACACAGCTGATCCGGAAGACCTGGATGTCGAAGATGAACACGAGGAATTGTCTTCAAATCAGACAGTC CGACGGCCTCAGGCATCACTGACGGACGCTGTGGCTACCTCGGGGGTGAGCACGCTAAGCTCCACTGTGT CCCATGACTCCCAGAATGCACACCGGTCGCTGAACGTGCAGCTTGGAAGGATGAAGATTGAAACTAATAG GTTACTTGAAGAGCTGGTTCGGAAAGAGAGAGAGTTACACGGCTCTCCATCAGGCATTGAGGAAAAAGAC CAAGAAATTAGGCACCTGAAGCTCAAGTCCCAGCCCATAGATATCCCTGGGTTTCCTGTGTGCCACCTGA ACTCTCCTGGCACGACCACGGAAGATTCTGAACTTCCTGGCTGGCTGAGAGAAAATGGAGCTGATGAAGA CAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98094. Forward Primer - name:098094_F_cDNA_Map3k5, sequence:GAGAAAGGATAGTGAGAGGCGA; Reverse Primer - name:098094_N_SP6_cDNA_Map3k5, sequence:GTGTCTTCATCAGCTCCATTTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11951 same embryo
	EMAGE:11954 same embryo
	EMAGE:11950 same embryo
	EMAGE:11952 same embryo
	EurExpress:euxassay_011097 same experiment
	MGI:4826082 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source