EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12095

Arpp21 cyclic AMP-regulated phosphoprotein, 21 ( MGI:107562)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008422. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008422_01	euxassay_008422_02	euxassay_008422_03	euxassay_008422_04

euxassay_008422_05	euxassay_008422_06	euxassay_008422_07	euxassay_008422_08	euxassay_008422_09

euxassay_008422_10	euxassay_008422_11	euxassay_008422_12	euxassay_008422_13	euxassay_008422_14

euxassay_008422_15	euxassay_008422_16	euxassay_008422_17	euxassay_008422_18	euxassay_008422_19

euxassay_008422_20	euxassay_008422_21	euxassay_008422_22	euxassay_008422_23	euxassay_008422_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12095_wholemount_strong.wlz

12095_wholemount_moderate.wlz

12095_wholemount_weak.wlz

12095_wholemount_possible.wlz

12095_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12095_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 22 23 24

upper leg muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 19 20 21 22 23 24

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hand

strong

regional

strong expression: see section 01 02 03

foot

strong

regional

strong expression: see section 04 05 06 20 21 22 23

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

strong

regional

strong expression: see section 11 12 13 14 15

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

spotted

moderate expression: see section 03 04 06 07 21 22 23

glossopharyngeal ix ganglion

moderate

spotted

moderate expression: see section 07 19

trigeminal v ganglion

moderate

spotted

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

moderate

spotted

moderate expression: see section 07 08 18

vestibulocochlear viii ganglion

moderate

spotted

moderate expression: see section 05 06 07 08 09 18 19 20

trigeminal v nerve

strong

spotted

strong expression: see section 10 moderate expression: see section 17 18

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

eye skeletal muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 20 21 22 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 15 16 17 18 19

tongue muscle

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

tail paraxial mesenchyme

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19

tail dorsal root ganglion

moderate

spotted

moderate expression: see section 07 08 09 10 11 12 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8403
Entity Detected:	Arpp21, cyclic AMP-regulated phosphoprotein, 21 ( MGI:107562)
Sequence:	sense strand is shown >T8403 CGTCGTCGGGTCTAAAAACTCTTTTTAATTTGGATCTGGGAGAATGTCTGAGCAAGGAGGACTGACTCCG ACCATACTGGAAGAAGGGCAGACGGAGCCAGAGTCTGCCCCAGAAAATGGCATCCTCAAGTCAGAAAGTC TGGATGAGGAGGAGAAGCTGGAACTGCAGCGGCGACTGGCGGCTCAGAACCAAGAGAGGAGAAAATCCAA GTCAGGAGCAGGCAAAGGGAAGCTGACCAGAAGTCTTGCTGTCTGTGAAGAGTCTTCAGCTAGATCTGGA GGGGAAAGTCACCAGGATCAGGAATCAATTCACTTACAGCTTTCCAGTTTCCCCAGCCTGCAAGAGGAGG ATAAATCTAGGAAGGATGATTCTGAGAGAGAAAAAGAAAAGGATAAGAACAGAGAGAAACTCTCTGAGAG ACCCAAGATCAGAATGTTATCAAAAGATTGCAGCCAAGAATACACAGATTCTACAGGCATAGACTTACAT GGGTTTCTAATTAACACGCTGAAGAACAATTCCAGGGACAGGATGATACTCTTGAAAATGGAGCAGGAAA TGATTGATTTCATTGCTGACAGCAATAACCACTATAAAAAGTTCCCCCAGATGTCATCCTATCAAAGGAT GCTGGTCCATCGGGTGGCAGCGTACTTTGGATTGGACCACAACGTGGATCAAACTGGAAAATCTGTCATC ATCAACAAGACCAGCAGCACCAGGATACCAGAGCAAAGGTTTTGTGAACATTTAAAAGATGAAAAAAGTG AAGAATCCCAGAAGCGGTTTATCTTGAAGCGAGATAACTCTAGTATTGATAAAGAAGACAATCAGTCAGT TTGCTCACAGGAAAGCCTATTTTTGGACAACAGTAGGCTCCAGGAAGACATGCACATATGCAATGAGACC TATAAGAAAAGACAGCTCTTTCGGGCTCACCGAGACAGCTCAGGAAGAACTTCCGGGAGCCGGCAGAGCA GCTCAGAGACTGAGCTCAGGTGGCCAGACCACCAGCGGGCTTGGAGCAGCACAGATTCGGACAGTTCCAA CCGCAATCTGAAGCCCACCATGACCAAGACGGCAAGTTTTGGGGGCATCACGGTGCTGACCAGGGGCGAC AGCACGTCCAGTACCAGGAGTGCTGGGAAACTGTCCAAAACAGGTTCTGAGTCTTCCAGCAGTGCGGGCT CCTCAGGATCACTGTCCCGCACCCACCCACAGAGCACGGCCCTGACCTCTAGCGTGGCAGCTGGTTCTCC TGGCTGTATGGCCTATTCAGAGAATGGAATGGGAGGCCAGGTTCCTCCCAGCAGCACCAGCTACATCCTC CTCCCACTTGAAAGTGCGACTGGCATCCCACCCGGAAGCATCCTTCTTAATCCACACACAGGTCAGCCCT TTGTGAACCCGGATGGAACGCCTGCGATATACAACCCTCCCGGAAGTCAGCAGACACTGCGTGGCACTGT TGGTGGGCAGCCCCAGCAACCTCCACAGCAGCAACCTTCGCCTCAGCCTCAGCAGCAGGTCCAGGCATCA CAGCCCCAGATGGCAGGGCCACTGGTCACTCAGTCTGTCCAGAGCCTGCAGCCATCTTCCCAGTCTGTGC AATATCCAGCGGTCTCTTTTCCTCCCCAGCATCTCCTGCCTATGTCGCCAACGCAGCACTTCCCCTTGAG AGAAGAACTGGCTGCCCAGTTCAGCCAGCTGAGCATGAGCCGGCAGTCCTCGGGAGACACTCCTGAACCT CCCTCTGGTACAGTCTACCCAGCCTCTCTCCTGCCACAAACGGCTCAGCCACAGAGTTATGTCATCACTT CTGCAGGCCAGCAGCTCTCTACAGGGGGCTTCTCGGACTCTGGCCCGCCCATCTCCCAGCAGGTCCTGCA GGCCCCGCCCTCTCCCCAGGGATTTGTACAGCAGCCTCCACCTGCACAGATGTCCGTCTATTACTACCCA TCTGGTCAGTACCCTACCTCAACCTCACAGCAGTACCGACCCCTCGCCTCAGTCCAGTACAGTGCTCAGC GGAGTCAGCAAATACCACAGACCACACAGCAAGCAGGTTACCAGCCAGTCTTGTCTGGTCAGCAGGGCTT CCAAGGCATGATGGGAGTACAGCAGTCAGCGCACAGCCAGGGTGTGATGAGCAGTCAGCAAGGAGCCCCG GTGCATGGTGTGATGGTCTCCTACCCAACCATGTCTTCTTATCAGGTGCCAATGACCCAAGGCTCTCAAG CAGTGCCCCAGCAGACATACCAACCACCAATCATGCTGCCCAGTCAGGCAGGCCAAGGGTCACTCCCAGC CACCGGAATGCCTGTGTACTGTAACGTCACACCGCCAAACCCTCAGAACAACCTAAGGTTGATGGGTCCA CACTGCCCCTCCAGCACGGTTCCTGTCATGTCCGCTAGTTGCAGAACAAACTGTGGGAACGTGAGCAACG CAGGCTGGCAGGTCAAGTTCTGAGAGCTCAGGCTGCCTGCTGTCCACTTCATCAGATGTTAACTCATGGC CTTCAAAGGAAGAGGAGACTGACGACTGGCTGAGGACTTCCATATACACTCAACGTGCAAACGATTGCTG CTGGTATTCTGTACAAACTAATCAAAGACTAATACACACATTAGCTGGTTAATGGTGCATAATTCCGTCA TGTCTGCTAGGTATGCCTTTCTAGCTTAGCTAGTGACATGAATTCATCAAGGTAAGATTTTCTCCTACCA CTGAATACCACTGTGTAGATTACAATATCCCTGATTCGGATTAGTTTTGTACTTCGTGTTGAGTTTGTGA TGCTAAAAGTATTTAAAAATATATATATAAACTAAATCACACGGTACCAAAGCTGTAATGGAAAAAGGAA AGAGTTAAGGAATGGAAGGAATAATTTATATACACTATAGAGTTTTCTTTTTTAAATGGATATATATGGT ATTGTAGTGTTTAATCCAAATAAAAGCTTATTTGACCTTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5686446 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5686446 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12097 same embryo
	EMAGE:12099 same embryo
	EMAGE:12096 same embryo
	EMAGE:12098 same embryo
	EurExpress:euxassay_008422 same experiment
	MGI:4823256 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source